Follicular Fluid Stimulation of 3β-Hydroxysteroid Dehydrogenase Activity in Vitro1

This work studied the effect of stresses produced by simulated gavage or gavage with 200 mmol/L HCl two hours before adrenal extraction, on the activities of the 11β-hydroxysteroid dehydrogenase 1 and 11β-hydroxysteroid dehydrogenase 2 isoforms present in the rat adrenal gland. These activities were determined on immediately prepared adrenal microsomes following incubations with 3H-corticosterone and NAD+ or NADP+. 11-dehydrocorticosterone was measured as an end-product by TLC, and controls were adrenal microsomes from rats kept under basal (unstressed) conditions. 11β-hydroxysteroid dehydrogenase 1 activity, but not 11β-hydroxysteroid dehydrogenase 2 activity, was increased under both stress-conditions. Homeostatically, the stimulation of 11β-hydroxysteroid dehydrogenase 1 activity would increase the supply of glucocorticoids. These, in turn, would activate the enzyme phenylethanolamine N-methyl transferase, thereby improving the synthesis of epinephrine as part of the stress-response.Key words: acidosis, adrenal, HSD, stress.

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Ovarian modulators of 11beta-hydroxysteroid dehydrogenase (11betaHSD) activity in follicular fluid from gonadotrophin-stimulated assisted conception cycles

Reproduction ◽

10.1530/rep.0.1240801 ◽

2002 ◽

pp. 801-812 ◽

Cited By ~ 13

Author(s):

LM Thurston ◽

DP Norgate ◽

KC Jonas ◽

C Chandras ◽

HJ Kloosterboer ◽

...

Keyword(s):

Follicular Fluid ◽

Rat Kidney ◽

Hydroxysteroid Dehydrogenase ◽

Controlled Ovarian Hyperstimulation ◽

Ovarian Hyperstimulation ◽

Assisted Conception ◽

Human Follicular Fluid ◽

Glucocorticoid Metabolism ◽

Stimulation Of

In the ovary, cortisol-cortisone interconversion is catalysed by isoforms of 11beta-hydroxysteroid dehydrogenase (11betaHSD). The objective of this study was to establish whether human follicular fluid (hFF), obtained after controlled ovarian hyperstimulation, contains paracrine modulators of 11betaHSD activity. Of 274 hFF samples tested for effects in rat kidney homogenates, 206 hFF samples significantly inhibited NADP(+)-dependent oxidation of cortisol within 1 h (by 11-67% of control 11betaHSD activity), whereas 42 hFF samples significantly stimulated 11betaHSD activity (16-210% increase relative to control). Although charcoal-stripping of hFF prevented the inhibition and potentiated the stimulation of NADP(+)-dependent cortisol oxidation in a renal homogenate, effects of individual hFF samples on NADP(+)-dependent cortisol oxidation were independent of intrafollicular progesterone concentrations. Hydrophilic fractions of hFF samples, isolated by C18 column chromatography, stimulated both the NADP(+)-dependent oxidation of cortisol (by 55+/-5%, n=98) and the NADPH-dependent reduction of cortisone (by 86+/-22%, n= 5). In contrast, the hydrophobic fractions of hFF (eluted at 65-85% methanol) inhibited both NADP(+)-dependent 11beta-dehydrogenase and NADPH-dependent 11-ketosteroid reductase activities (by 63+/-2% and 74+/-4%, respectively). None of the C18 column fractions of 50 hFF samples had any significant effect on NAD(+)-dependent 11beta-dehydrogenase activities. The hydrophobic inhibitors of NADP(H)-dependent cortisol-cortisone metabolism did not co-elute with several candidate compounds (prostaglandins E(2) and F(2alpha), cortisol, cortisone, oestradiol, testosterone, progesterone, pregnenolone or cholesterol). Hence, hFF aspirated from women undergoing controlled ovarian hyperstimulation for assisted conception contains both hydrophilic stimuli and hydrophobic inhibitors of glucocorticoid metabolism which appear to be selective for the NADP(H)-dependent, type 1 isoform of 11betaHSD.

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Inhibition of Δ5-3β hydroxysteroid dehydrogenase activity in pre-implantation mouse embryos and their implantation by sheep ovarian follicular fluid peptide

Acta Endocrinologica ◽

10.1530/acta.0.1150183 ◽

1987 ◽

Vol 115 (2) ◽

pp. 183-188 ◽

Cited By ~ 3

Author(s):

N.J. Joshi ◽

S. B. Moodbidri ◽

Tarala D. Nandedkar

Keyword(s):

Dehydrogenase Activity ◽

Follicular Fluid ◽

Hydroxysteroid Dehydrogenase ◽

Mouse Embryos ◽

Uterine Lumen ◽

Pregnant Mice ◽

Ovarian Follicular Fluid

Abstract. The activity of Δ5-3β hydroxysteroid dehydrogenase (Δ5-3β HSD) in pre-implantation mouse embryos was inhibited following their prior incubation with partially purified sheep ovarian follicular fluid peptide (OFFP). OFFP, 5 μl, injected into the uterine lumen of day 4 pregnant mice impaired the ensuing implantation of embryos. Our observations suggest that OFFP may inhibit steroid metabolizing activity in pre-implantation embryos and thereby interfere with the process of implantation.

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THE INFLUENCE OF HAEMOGLOBIN-S AND G6PD DEFICIENCY ON THE ACTIVITY OF THE 17β-HYDROXYSTEROID DEHYDROGENASE OF INTACT HUMAN ERYTHROCYTES

Acta Endocrinologica ◽

10.1530/acta.0.0750793 ◽

1974 ◽

Vol 75 (4) ◽

pp. 793-800

Author(s):

A. O. Sogbesan ◽

O. A. Dada ◽

B. Kwaku Adadevoh

Keyword(s):

Enzyme Activity ◽

Dehydrogenase Activity ◽

Sex Steroids ◽

G6pd Deficiency ◽

Incubation Medium ◽

Hydroxysteroid Dehydrogenase ◽

G6pd Activity ◽

Reverse Transformation ◽

Oxidative Transformation ◽

Haemoglobin S

ABSTRACT The 17β-hydroxysteroid dehydrogenase activity in intact erythrocytes of Nigerian patients, in particular with regard to haemoglobin genotypes and G6PD* activity was studied. The G6PD activity of the erythrocyte did not affect the oxidative transformation of testosterone to androstenedione and of oestradiol to oestrone. The reduction (reverse transformation) was inhibited in G6PD-deficient erythrocytes but this inhibition was offset by the addition of 0.025 m glucose to the incubation medium. The per cent oxidation transformation of testosterone was higher in Hb-AA than in Hb-SS erythrocytes. It is suggested that the differences may be a result of either lower enzyme activity in the Hb-SS erythrocytes or of differences in the uptake and possibly binding of sex steroids by intact Hb-SS and Hb-AA erythrocytes.

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Δ4-3/5-HYDROXYSTEROID DEHYDROGENASE ACTIVITY IN RAT LIVER: INTRACELLULAR DISTRIBUTION AND SEX DEPENDENCY

Acta Endocrinologica ◽

10.1530/acta.0.077s038 ◽

1974 ◽

Vol 77 (1_Suppl) ◽

pp. S38

Author(s):

E.R. Lax ◽

H. Schriefers

Keyword(s):

Dehydrogenase Activity ◽

Rat Liver ◽

Hydroxysteroid Dehydrogenase ◽

Intracellular Distribution ◽

Sex Dependency

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ACUTE ENZYME HISTOCHEMICAL CHANGES IN THE ZONA GLOMERULOSA OF THE RAT ADRENAL CORTEX

Acta Endocrinologica ◽

10.1530/acta.0.0590508 ◽

1968 ◽

Vol 59 (3) ◽

pp. 508-518

Author(s):

J. D. Elema ◽

M. J. Hardonk ◽

Joh, Koudstaal ◽

A. Arends

Keyword(s):

Peritoneal Dialysis ◽

Succinate Dehydrogenase ◽

Dehydrogenase Activity ◽

Adrenal Cortex ◽

Isocitrate Dehydrogenase ◽

Hydroxysteroid Dehydrogenase ◽

Zona Glomerulosa ◽

Acute Changes ◽

Glucose 6 Phosphate Dehydrogenase ◽

Histochemical Changes

ABSTRACT Acute changes in glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase activity in the zona glomerulosa of the rat adrenal cortex were induced by peritoneal dialysis with 5 % glucose. Although less clear, the activity of 3β-ol-hydroxysteroid dehydrogenase also seemed to increase as well. No changes were seen in the activity of succinate dehydrogenase. Dialysis with 0.9 % NaCl had no effect on any of the enzymes investigated. The possible significance of these observations is discussed.

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