Effects of Individual and Combined Treatment with Prostaglandins E2 and F2α on Progesterone Secretion by Ovine Luteal Cells Supplemented with Homologous Serum Lipoproteins in Vitro1

The hypothesis that epinephrine (noradrenaline, NA) enhances utilisation of high-density lipoproteins (HDL) by bovine luteal cells and that this process involves phospholipase (PL) C and protein kinase (PK) C intracellular pathway was tested. Luteal cells from days 2-4, 5-10 or 11-17 of the oestrous cycle were pre-incubated for 20h. Subsequently DMEM/Ham's F-12 medium was replaced by fresh medium and the cells were treated for 6 h as follows: In Experiment I with HDL (5-75μg cholesterol per ml), NA, isoprenaline (ISO) or luteinising hormone (LH). In Experiment II cells were incubated for further 24h in deficient medium (without FCS) and next treated as in Experiment I. In Experiment III cells were stimulated with NA, ISO or LH alone and together with HDL. In Experiment IV cells were treated with PLC inhibitor (U-73122) or with PKC inhibitor (staurosporine) or stimulator (phorbol 12-myristrate 13-acetate) and with either NA, insulin or LH. Only luteal cells from days 5-10 of the cycle responded on HDL and β-mimetics (P<0.05). LH stimulated progesterone secretion from the luteal cells during all stages of the cycle (P<0.001). Cells incubated in deficient medium and supplemented with HDL secreted as much progesterone as those stimulated by LH in all stages of the cycle. Beta-mimetics were unable to enhance the stimulatory effect of HDL. Blockade of PLC had no influence on progesterone secretion from cells treated with either NA or LH, but this did impair the stimulatory effect of insulin (P<0.05). Similarly, blockade of PKC by staurosporine impaired (P<0.05) the effect of insulin only but not that observed after LH or NA treatment. We suggest that: (a) noradrenergic stimulation does not enhance utilisation of cholesterol from HDL for progesterone secretion; (b) the fasting of luteal cells seems to activate enzymes responsible for the progesterone synthesis; (c) effect of NA on progesterone secretion from luteal cells does not involve the PLC-PKC pathway.

Download Full-text

The Effect of Uterine Flushings and Endometrial Protein Fractions on Progesterone Secretion by Porcine Luteal Cells

Acta Veterinaria Brno ◽

10.2754/avb198655030163 ◽

1986 ◽

Vol 55 (3) ◽

pp. 163-171

Author(s):

Jadwiga Przala ◽

Z. Luberda ◽

A. Grazul ◽

T. Wiesak ◽

J. Kotwica

Keyword(s):

Protein Fractions ◽

Luteal Cells ◽

Progesterone Secretion ◽

Porcine Luteal Cells

Download Full-text

Notch Signaling Pathway Regulates Progesterone Secretion in Murine Luteal Cells

Reproductive Sciences ◽

10.1177/1933719115572480 ◽

2015 ◽

Vol 22 (10) ◽

pp. 1243-1251 ◽

Cited By ~ 10

Author(s):

Jing Wang ◽

Shuangmei Liu ◽

Lichao Peng ◽

Qiming Dong ◽

Riqiang Bao ◽

...

Keyword(s):

Notch Signaling ◽

Signaling Pathway ◽

Notch Signaling Pathway ◽

Luteal Cells ◽

Progesterone Secretion

Download Full-text

Increasing progesterone secretion in human granulosa-luteal cells induced by human follicular fluid*,**

Human Reproduction ◽

10.1093/oxfordjournals.humrep.a137872 ◽

1993 ◽

Vol 8 (1) ◽

pp. 46-52 ◽

Cited By ~ 3

Author(s):

Shalom Bar-Ami ◽

Camellia Khoury ◽

Efrat Zlotkin ◽

Joseph M. Brandes

Keyword(s):

Follicular Fluid ◽

Human Follicular Fluid ◽

Luteal Cells ◽

Progesterone Secretion

Download Full-text

Characteristics of bovine luteal cells in culture: morphology, proliferation and progesterone secretion in different media and effects of LH, dibutyryl cyclic AMP, antioxidants and insulin

Journal of Endocrinology ◽

10.1677/joe.0.1040355 ◽

1985 ◽

Vol 104 (3) ◽

pp. 355-361 ◽

Cited By ~ 24

Author(s):

P. J. O'Shaughnessy ◽

D. C. Wathes

Keyword(s):

Cyclic Amp ◽

Doubling Time ◽

Rapid Decline ◽

Dibutyryl Cyclic Amp ◽

Functional Characteristics ◽

Luteal Cells ◽

Progesterone Secretion ◽

Adult Bovine Serum ◽

Culture Morphology ◽

Altered Cell

ABSTRACT The morphological and functional characteristics of cultured bovine luteal cells were examined. Dispersed luteal cells were cultured in either Ham's F12 medium or Dulbecco's Modified Eagle's Medium (DMEM), plus 10% adult bovine serum. After 18 h of culture, fibronectin was observed to be associated with some cells showing histochemical 3β-hydroxysteroid dehydrogenase (3β-HSD) activity. In DMEM, cells adopted a fibroblast-like appearance with a doubling time of 34 h while in Ham's F12, cells retained an epithelial-like morphology with a doubling time of 91 h. Dibutyryl cyclic AMP (dbcAMP) but not LH slowed proliferation and altered cell morphology in DMEM. Addition of insulin and dbcAMP was required to maintain 3β-HSD activity in cells cultured for more than 3 days. Progesterone secretion was significantly greater in Ham's F12 than in DMEM although there was a rapid decline in basal secretion during culture in either medium. Both LH and dbcAMP stimulated progesterone secretion although the effects of dbcAMP were significantly greater than those of LH after 18 h of culture. Addition of insulin significantly enhanced basal, LH-stimulated and dbcAMP-stimulated progesterone secretion after 3 days of culture whereas several antioxidants were without effect. These studies indicate that the morphological and functional characteristics of cultured luteal cells may be best maintained in Ham's F12 plus dbcAMP and insulin. J. Endocr. (1985) 104, 355–361

Download Full-text

Hypokalemia alters sex hormone and gonadotropin levels: evidence that FSH may be required for luteinization

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1998.275.6.e1037 ◽

1998 ◽

Vol 275 (6) ◽

pp. E1037-E1045

Author(s):

Francisco Tejada ◽

Asunción Cremades ◽

Manuel Avilés ◽

Maria T. Castells ◽

Rafael Peñafiel

Keyword(s):

Luteinizing Hormone ◽

Follicle Stimulating Hormone ◽

Combined Treatment ◽

Gonadotropin Releasing Hormone ◽

Sex Hormone ◽

Plasma Progesterone ◽

Progesterone Secretion ◽

Potassium Gradient ◽

Deficient Mice ◽

Estradiol Levels

Hypokalemia produced different effects on steroid sex hormone concentrations in plasma and ovary in the mouse. Estradiol levels were slightly increased, whereas circulating progesterone was markedly decreased in all estrous periods. The preovulatory surge of gonadotropins and the secondary surge of follicle-stimulating hormone (FSH) at estrus were also decreased, but basal levels of both gonadotropins were unaffected. Supplementation with luteinizing hormone (LH), FSH, or gonadotropin-releasing hormone (GnRH) at proestrus rapidly normalized plasma and ovarian progesterone levels at this stage of the estrous cycle. Plasma progesterone levels at diestrus were restored only by combined treatment, at the periovulatory stage, with LH and FSH or GnRH but not by LH or FSH alone. The results demonstrate a lack of steroidogenic activity in the corpus luteum of the potassium-deficient mice and, furthermore, that FSH plays an important role in luteinization in the hypokalemic mice. We conclude that alteration of the transcellular potassium gradient may affect the regulation of the periovulatory surge of gonadotropins and progesterone secretion, probably by altering the release of GnRH from the hypothalamus. In addition, the results suggest that FSH may play a certain role as a luteotropic hormone in mice.

Download Full-text