scholarly journals Development of a Culture Medium (BECM-3) for Porcine Embryos: Effects of Bovine Serum Albumin and Fetal Bovine Serum on Embryo Development

1996 ◽  
Vol 55 (5) ◽  
pp. 1069-1074 ◽  
Author(s):  
J. R. Dobrinsky ◽  
L. A. Johnson ◽  
D. Rath
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Embryo Development ◽  
Bovine Serum ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

Influence of l-carnitine on lipid metabolism of buffalo cumulus-oocyte complexes matured in either fetal bovine serum or fatty acid-free bovine serum albumin

2020 ◽  
Vol 158 ◽  
pp. 382-390
Author(s):  
Diego Fernando Dubeibe Marin ◽  
Nathalia Nogueira da Costa ◽  
Priscilla di Paula Bessa Santana ◽  
Eduardo Baia de Souza ◽  
Sebastião Tavares Rolim filho ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Fetal Bovine

Electrochemical detection of N-homocysteinylated BSA in the fetal bovine serum medium

RSC Advances ◽  
2015 ◽  
Vol 5 (7) ◽  
pp. 4774-4779 ◽  
Author(s):  
Ece Eksin ◽  
Arzum Erdem
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Electrochemical Detection ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Homocysteine Thiolactone ◽  
Passive Adsorption ◽  
Fetal Bovine ◽  
Fetal Bovine Serum Medium

The immobilization of bovine serum albumin (BSA), homocysteine-thiolactone (HTL) andN-homocysteinylated BSA (N-Hcy-BSA) onto the surface of each PGE was performed by passive adsorption and the electrochemical detection of these components was investigated individually.

scholarly journals PS48 can replace bovine serum albumin in pig embryo culture medium, and improve in vitro embryo development by phosphorylating AKT

2015 ◽  
Vol 82 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Lee D. Spate ◽  
Alana Brown ◽  
Bethany K. Redel ◽  
Kristin M. Whitworth ◽  
Randall S. Prather
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Embryo Development ◽  
Embryo Culture ◽  
Bovine Serum ◽  
Culture Medium ◽  
Embryo Culture Medium

scholarly journals Bovine serum albumin as an immunogenic carrier facilitating the development of hapten-specific monoclonal antibodies

2020 ◽  
Author(s):  
Ruotong Zhao ◽  
Mingjun Jiang
Keyword(s):  
Monoclonal Antibodies ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Limit Of Detection ◽  
Fetal Bovine Serum ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Common Misconception ◽  
Fetal Bovine

AbstractThere is a common misconception that the generation of hapten-specific monoclonal antibodies (mAbs) requires the use of a heterologous conjugate to ensure carrier-specific antibodies not being detected. In this study, salbutamol (SAL) was used as a model hapten to exhibit the benefits of bovine serum albumin (BSA) as a carrier for developing hapten-specific mAbs. SAL-BSA conjugate would serve as both an immune antigen and a screening antigen during the preparation of SAL-specific mAbs. Six hybridomas were identified to secret mAbs specific for free SAL with minor or negligible cross-reactivity with other β-agonists. Meanwhile, none of hybrodomas secreting anti-BSA antibodies were screened out even though the fetal bovine serum (FBS) added to the medium decreased from 10% to 1% (v/v). Based on one of the six mAbs, 3F12, a direct competitive enzyme-linked immunosorbent assay (dcELISA) was developed for meausring SAL. Under the optimized assay, the quantitative working range was from 312.5 to 20,000 pg/mL (R2 = 0.9959), with a limit of detection (LOD) of 142.9 pg/mL. The results showed that BSA is an efficient and suitable protein carrier for facilitating the development of hapten-specific mAbs.

scholarly journals TINGKAT PERKEMBANGAN AWAL EMBRIO SAPI IN VITRO MENGGUNAKAN MEDIA TUNGGAL BERBAHAN DASAR TISSUE CULTURE MEDIUM (TCM) 199

2013 ◽  
Vol 7 (2) ◽  
Author(s):  
Mohamad Agus Setiadi ◽  
Ni Wayan Kurniani Karja
Keyword(s):  
Tissue Culture ◽  
Bovine Serum Albumin ◽  
Chorionic Gonadotropin ◽  
Bovine Serum ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Tissue Culture Medium ◽  
Serum Gonadotropin ◽  
Fetal Bovine

Penelitian ini bertujuan mengetahui kemampuan perkembangan awal embrio sapi in vitro menggunakan media tunggal untuk maturasi, fertilisasi, dan kultur berbahan dasar tissue culture medium (TCM) 199. Oosit sapi dikumpulkan dari rumah potong hewan dengan teknik aspirasi dan diklasifikasikan berdasarkan kekompakan sel kumulus dan sitoplasma yang homogen. Oosit dimaturasi pada medium TCM 199 yang disuplementasi dengan 10 IU/ml pregnant mare’s serum gonadotropin (PMSG), 10 IU/ml human chorionic gonadotropin (hCG), dan 10% fetal bovine serum (FBS), dilakukan selama 24 jam pada inkubator 5% CO2, 39 C. Fertilisasi dilakukan pada dua media yang berbeda yaitu media rutin fertilisasi dan media berbahan dasar TCM 199 dengan suplemen bovine serum albumin (BSA) dan heparin. Setelah fertilisasi, kumulus sel dihilangkan (denudasi), kemudian dikultur pada media TCM 199 yang disuplementasi dengan asam amino esensial dan non-esensial serta 10% FBS selama 3 hari. Hasil penelitian menunjukkan tingkat maturasi oosit pada sistem yang digunakan mampu mendukung 81,5% oosit mencapai tahap metafase II (M-II). Tingkat pembelahan embrio lebih tinggi pada media rutin dibandingkan dengan media TCM 199 yakni masing-masing 44,4 dan 23,2%. Jumlah embrio tahap 4-8 sel pada kedua perlakuan tidak berbeda nyata. Dapat disimpulkan media tunggal berbasis TCM dapat digunakan untuk produksi embrio in vitro.

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