Insect Cell Culture Medium Supplementation with Fetal Bovine Serum and Bovine Serum Albumin: Effects on Baculovirus Adsorption and Infection Kinetics

2002 ◽  
Vol 18 (4) ◽  
pp. 855-861 ◽  
Author(s):  
L. Maranga ◽  
A.S. Coroadinha ◽  
M.J.T. Carrondo
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Insect Cell ◽  
Bovine Serum ◽  
Culture Medium ◽  
Fetal Bovine Serum ◽  
Insect Cell Culture ◽  
Cell Culture Medium ◽  
Fetal Bovine ◽  
Medium Supplementation

scholarly journals Elucidation of the Interaction between Flavan-3-ols and Bovine Serum Albumin and Its Effect on Their In-Vitro Cytotoxicity

Molecules ◽  
2019 ◽  
Vol 24 (20) ◽  
pp. 3667
Author(s):  
Yasuyuki Fujii ◽  
Yoshitomo Suhara ◽  
Yusuke Sukikara ◽  
Tomohiro Teshima ◽  
Yoshihisa Hirota ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
In Vitro Cytotoxicity ◽  
Binding Energies ◽  
Docking Simulations ◽  
Fetal Bovine

Flavan-3-ols (FLs), specifically catechin and its oligomer B-type procyanidins, are suggested to potently bind to bovine serum albumin (BSA). We examined the interaction between BSA and FLs by fluorescence quenching and found the following order of binding activities to BSA: cinnamtannin A2 (A2; tetramer) > procyanidin C1 (C1; trimer) ≈ procyanidin B2 (B2, dimer) > (−)epicatechin (EC, monomer). Docking simulations between BSA and each compound at the binding site showed that the calculated binding energies were consistent with the results of our experimental assay. FLs exerted cytotoxicity at 1000 μg/mL in F11 cell culture with fetal bovine serum containing BSA. In culture containing serum-free medium, FLs exhibited significant cell proliferation at 10−4 μg/mL and cytotoxicity was observed at concentrations greater than 10 μg/mL. Results of this study suggest that interactions between polyphenols and BSA should be taken into account when evaluating procyanidin in an in vitro cell culture system.

scholarly journals Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species

2008 ◽  
Vol 74 (16) ◽  
pp. 5224-5227 ◽  
Author(s):  
Tanja Riess ◽  
Florian Dietrich ◽  
Katja V. Schmidt ◽  
Patrick O. Kaiser ◽  
Heinz Schwarz ◽  
...  
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Expression Patterns ◽  
Insect Cell Culture ◽  
Host Cells ◽  
Cell Culture Medium ◽  
Bacterial Protein ◽  
Bartonella Species ◽  
Slow Growing

ABSTRACT Human- and animal-pathogenic Bartonella species are fastidious and slow-growing bacteria difficult to isolate and cultivate. We describe a novel, easy-to-prepare liquid medium for the fast and reliable growth of several Bartonella spp. that does not affect bacterial protein expression patterns or interactions with host cells.

Gas Bubbles and Their Influence on Microorganisms

1998 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Jeffrey J. Chalmers
Keyword(s):  
Cell Culture ◽  
Insect Cell ◽  
Culture Medium ◽  
Cell Damage ◽  
Gas Bubbles ◽  
Insect Cell Culture ◽  
Review Article ◽  
Cell Culture Medium

A review of the interactions of microorganisms and bubbles is presented. Particular emphasis is given to the damage that results when gas bubbles are introduced into animal and insect cell culture medium. The mechanism of this damage is presented as well as a discussion of the protective additives that have been found to reduce cell damage from gas bubbles. This review article has 43 references.

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