A histomorphometric study of alveolar bone healing in rats fed a boron‐deficient diet

The management of critical-size bone defects is still demanding. Recently, autologous platelet concentrates in combination with bone substitute have been applied and reported in a few studies. Our aim is to report the healing of a critical-size alveolar bone defect treated with a new bone regeneration technique by means of L-PRF and L-PRF blocks. A 45-year-old woman presented a large cystic lesion; the extraction of three teeth, a cyst removal procedure, and bone regeneration procedures with L-PRF and L-PRF blocks were planned. The L-PRF block was prepared by mixing a bone substitute with a piece of L-PRF membrane and liquid fibrinogen. Additionally, after bone healing an implant-based rehabilitation was optimally performed. On the basis of the positive results, in terms of bone healing and tissue regeneration in a large bone defect, the application of L-PRF and L-PRF blocks, in agreement with the scarce literature, is suggested as a feasible procedure in selected cases.

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Effect of age on alveolar bone turnover adjacent to maxillary molar roots in male rats: A histomorphometric study

Archives of Oral Biology ◽

10.1016/j.archoralbio.2006.06.012 ◽

2007 ◽

Vol 52 (1) ◽

pp. 44-50 ◽

Cited By ~ 20

Author(s):

Yasuko Misawa ◽

Toru Kageyama ◽

Keita Moriyama ◽

Saburo Kurihara ◽

Hiroshi Yagasaki ◽

...

Keyword(s):

Bone Turnover ◽

Alveolar Bone ◽

Male Rats ◽

Histomorphometric Study ◽

Maxillary Molar ◽

Effect Of Age

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Histomorphometric Study of Bone Healing Around Laminar Implants in Experimental Diabetes

Implant Dentistry ◽

10.1097/00008505-200009020-00006 ◽

2000 ◽

Vol 9 (2) ◽

pp. 143-149 ◽

Cited By ~ 36

Author(s):

Maximo Juan Giglio ◽

Graciela Giannunzio ◽

Daniel Olmedo ◽

María Beatriz Guglielmotti

Keyword(s):

Bone Healing ◽

Experimental Diabetes ◽

Histomorphometric Study

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Effect of platelet-rich plasma on implant bone defects in rabbits through the FAK/PI3K/AKT signaling pathway

Open Life Sciences ◽

10.1515/biol-2019-0034 ◽

2019 ◽

Vol 14 (1) ◽

pp. 311-317

Author(s):

Wei Liu ◽

Ben Chen ◽

Youyang Zheng ◽

Yuehua Shi ◽

Zhuojin Shi

Keyword(s):

Growth Factor ◽

Bone Healing ◽

Alveolar Bone ◽

Platelet Rich Plasma ◽

Healing Process ◽

Bone Defects ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Tissue Healing ◽

Bone Healing Process

AbstractPlatelet-rich plasma (PRP) has been shown to be a beneficial growth factor for bone tissue healing and is used in implantology. The aim of this study was to investigate the effects of PRP on bone defects in rabbits. Twenty rabbits were used to establish the implant bone defect model in this study. An intrabony defect (5mm × 5mm × 3mm) was created in alveolar bone in the lower jar of each rabbit. The wound was treated with PRP. The expression of platelet-derived growth factor BB (PDGFBB) was assessed by enzyme-linked immunosorbent assay (ELISA). Focal adhesion kinase (FAK) and related phosphatidylinositol 3-kinase (PI3K)/AKT (protein kinase B) levels were measured by Western blot. The results show that PRP could significantly improve the bone healing process when compared with control, and 10% PRP could markedly increase fibroblast proliferation 48-h post treatment. PDGFBB was higher in the PRP group than that in the control group. PRP treatment also could elevate the phosphorylation of FAK and PI3K/AKT, although the inhibitor of PDGFR could reverse this trend. These results suggest that PRP treatment improves the bone healing process through the FAK/PI3K/AKT pathway.

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Retracted: Influence of Biphasic β-TCP with and without the use of collagen membranes on bone healing of surgically critical size defects. A radiological, histological, and histomorphometric study

Clinical Oral Implants Research ◽

10.1111/clr.12258 ◽

2013 ◽

Vol 25 (11) ◽

pp. 1228-1238 ◽

Cited By ~ 14

Author(s):

Jose L. Calvo-Guirado ◽

Maria P. Ramírez-Fernández ◽

Rafael A. Delgado-Ruíz ◽

Jose E. Maté-Sánchez ◽

Pablo Velasquez ◽

...

Keyword(s):

Bone Healing ◽

Critical Size ◽

Histomorphometric Study ◽

Critical Size Defects ◽

Collagen Membranes

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Influence of Homemade and Commercial Fibrin Sealant on Alveolar Bone Healing - an Experimental Study.

Blood ◽

10.1182/blood.v104.11.4110.4110 ◽

2004 ◽

Vol 104 (11) ◽

pp. 4110-4110

Author(s):

Maria Elvira P. Correa ◽

Oslei P. Almeida ◽

Danyel Peres ◽

Marcelo C. Alves ◽

Fernando F. Costa ◽

...

Keyword(s):

Bone Formation ◽

Bone Healing ◽

Fibrin Sealant ◽

Factor Xiii ◽

Alveolar Bone ◽

Healing Process ◽

Control Group ◽

Image Analysis System ◽

New Bone Formation ◽

Fibrin Sealants

Abstract The beneficial aspects of fibrin sealants for soft tissues are well documented, but studies of their direct influence on bone healing and their effectiveness in augmenting bone graft healing have produced conflicting results. The aim of this study was to evaluate the influence of fibrin sealants (FS) in the alveolar bone healing process during a period of up to 28 days. Seventy-five Wistar rats were submitted to a superior incisor extraction after intramuscular anesthesia (kentamine chloridrate 10% – 10mg/kg and tiazina chloridrate-5mg/kg). The rats were divided into tree different groups and the sealants were introduced into the alveolar bone. The first group (25) received human homemade fibrin glue, the second group (25) FS associated to factor XIII and aprotinin (Beriplast; Aventis-Beringher) and the third group (25) was the control. Animals were sacrificed by prolonged diethyl inhalation on days 7, 14 and 28 after surgery. Animal craniums were dissected and submitted to a decalcification, and preparated for H&E light microscopy. The morphometric study was performed by means of an interactive computerized image analysis system KS400 (Zeiss, Jena). New bone formation was carefully delimited in four different alveolar regions (apical, two middle areas and cervical) of each specimen. The data were statistically analyzed using multiple regression, ANOVA and Tukey test. Results showed that the amount of alveolar bone formation (μm) in the control group and commercial sealant was statistically similar. However, alveolus receiving homemade sealant presented less amount of new bone formation comparing to commercial sealant and control group (p=0.0034) (figure 1). The present study demonstrated that homemade fibrin sealant delays osteogenic formation. Commercial sealant did not improve alveolar repair however, the amount of new bone formation was slightly higher comparing to the control, probably due to the factor XIII present in this sealant. Figure Figure

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