Immunohistochemestry for nerve morphometry?

Problem The prognosis of bipolar electrocautery nerve injury is unpredictable because few experiments compare it to characterized nerve injury models. Methods 16 Lewis rats were randomized to sciatic crush or bipolar cautery and at 21 days evaluated with nerve morphometry and walking tack analysis. Double transgenic Thy1-CFP/S100-GFP mice were used to serially image axonal regeneration and Schwann cells (SC) over time following injury. Results In rats, bipolar cautery injury shows greater disruption of myelin and neurofilament architecture at the injury site. There is decreased total nerve fiber counts distal to the injury (p<0.05). Walking track analysis demonstrates functional recovery after crush, but not after cautery injury. Serial imaging of mice, shows axonal regeneration starting at week 1 after crush, but late, partial axonal regeneration in the cautery group; these findings were reflected in endplate reinnervation at 42 days. Conclusion Results suggest that a Sunderland type 3 injury, characterized by slow, variable, incomplete recovery, results from a bipolar electrocautery injury. Significance Bipolar cautery injuries can be observed without immediate surgical intervention.

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Interpreting nerve morphometry data after experimental traumatic lesions

Journal of Neuroscience Methods ◽

10.1016/0165-0270(94)00156-b ◽

1995 ◽

Vol 58 (1-2) ◽

pp. 29-37 ◽

Cited By ~ 42

Author(s):

Luis de Medinaceli

Keyword(s):

Traumatic Lesions ◽

Nerve Morphometry

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Two nerve morphometry methods performed through the same computer program: are they comparable?

The FASEB Journal ◽

10.1096/fasebj.26.1_supplement.725.19 ◽

2012 ◽

Vol 26 (S1) ◽

Author(s):

Antônio Paulo Costa Bilego Neto ◽

Fernando Braga Cassiano Silveira ◽

Luciana Sayuri Sanada ◽

Valéria Paula Sassoli Fazan

Keyword(s):

Computer Program ◽

Nerve Morphometry

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A structured program in BASIC for the analysis of peripheral nerve morphometry

Journal of Neuroscience Methods ◽

10.1016/0165-0270(82)90057-7 ◽

1982 ◽

Vol 5 (1-2) ◽

pp. 103-120 ◽

Cited By ~ 11

Author(s):

A.H. Pullen

Keyword(s):

Peripheral Nerve ◽

Structured Program ◽

Nerve Morphometry

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Decellularized Human Umbilical Artery Used as Nerve Conduit

Bioengineering ◽

10.3390/bioengineering5040100 ◽

2018 ◽

Vol 5 (4) ◽

pp. 100 ◽

Cited By ~ 2

Author(s):

Ioanna Gontika ◽

Michalis Katsimpoulas ◽

Efstathios Antoniou ◽

Alkiviadis Kostakis ◽

Catherine Stavropoulos-Giokas ◽

...

Keyword(s):

Umbilical Artery ◽

Complete Recovery ◽

Nerve Fibers ◽

Segmental Defect ◽

Sprague Dawley ◽

Sprague Dawley Rats ◽

Human Umbilical Artery ◽

Microsurgical Techniques ◽

Sciatic Functional Index ◽

Nerve Morphometry

Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the use of the decellularized human umbilical artery (hUA) as nerve guidance conduit. A segmental peripheral nerve injury was created in 24 Sprague–Dawley rats. The animals were organized into two experimental groups with different forms of repair: decellularized hUA (n = 12), and autologous nerve graft (n = 12). Sciatic faction index and gastrocnemius muscle values were calculated for functional recovery evaluation. Nerve morphometry was used to analyze nerve regeneration. Results showed that decellularized hUAs after implantation were rich in nerve fibers and characterized by improved Sciatic Functional index (SFI) values. Decellularized hUA may support elongation and bridging of the 10 mm nerve gap.

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Reproducibility in Nerve Morphometry: Comparison between Methods and among Observers

BioMed Research International ◽

10.1155/2013/682849 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 4

Author(s):

Antônio Paulo da Costa Bilego Neto ◽

Fernando Braga Cassiano Silveira ◽

Greice Anne Rodrigues da Silva ◽

Luciana Sayuri Sanada ◽

Valéria Paula Sassoli Fazan

Keyword(s):

Myelin Sheath ◽

Computer Software ◽

Myelinated Fiber ◽

Adult Rats ◽

Single Experiment ◽

Myelinated Fibers ◽

Manual Intervention ◽

Reproducible Method ◽

Semiautomated Method ◽

Nerve Morphometry

We investigated the reproducibility of a semiautomated method (computerized with manual intervention) for nerve morphometry (counting and measuring myelinated fibers) between three observers with different levels of expertise and experience with the method. Comparisons between automatic (fully computerized) and semiautomated morphometric methods performed by the same computer software using the same nerve images were also performed. Sural nerves of normal adult rats were used. Automatic and semiautomated morphometry of the myelinated fibers were made through the computer software KS-400. Semiautomated morphometry was conducted by three independent observers on the same images, using the semiautomated method. Automatic morphometry overestimated the myelin sheath area, thus overestimating the myelinated fiber size and underestimating the axon size. Fiber distributions overestimation was of 0.5 μm. For the semiautomated morphometry, no differences were found between observers for myelinated fiber and axon size distributions. Overestimation of the myelin sheath size of normal fibers by the fully automatic method might have an impact when morphometry is used for diagnostic purposes. We suggest that not only semiautomated morphometry results can be compared between different centers in clinical trials but it can also be performed by more than one investigator in one single experiment, being a reliable and reproducible method.

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