Regulation of Inducible Nitric Oxide Synthase (iNOS) by Cyclin‐Dependent Kinase 7 (CDK7) in Endothelial Cells

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Shruthi Shetty ◽  
Amelia M. Spaulding ◽  
Cassadie Baker ◽  
Stephen H. Baine ◽  
Dale G. Hoyt
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Cyclin Dependent Kinase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Endothelial Hyper-Permeability Induced by T1D Sera Can be Reversed by iNOS Inactivation

2020 ◽  
Vol 21 (8) ◽  
pp. 2798
Author(s):  
Alessandra Cazzaniga ◽  
Roberta Scrimieri ◽  
Elisa Giani ◽  
Gian Vincenzo Zuccotti ◽  
Jeanette A. M. Maier
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Endothelial Permeability ◽  
Vascular Wall ◽  
High Morbidity ◽  
Lipid Uptake ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

Type 1 Diabetes Mellitus (T1D) is associated with accelerated atherosclerosis that is responsible for high morbidity and mortality. Endothelial hyperpermeability, a feature of endothelial dysfunction, is an early step of atherogenesis since it favours intimal lipid uptake. Therefore, we tested endothelial leakage by loading the sera from T1D patients onto cultured human endothelial cells and found it increased by hyperglycaemic sera. These results were phenocopied in endothelial cells cultured in a medium containing high concentrations of glucose, which activates inducible nitric oxide synthase with a consequent increase of nitric oxide. Inhibition of the enzyme prevented high glucose-induced hyperpermeability, thus pointing to nitric oxide as the mediator involved in altering the endothelial barrier function. Since nitric oxide is much higher in sera from hyperglycaemic than normoglycaemic T1D patients, and the inhibition of inducible nitric oxide synthase prevents sera-dependent increased endothelial permeability, this enzyme might represent a promising biochemical marker to be monitored in T1D patients to predict alterations of the vascular wall, eventually promoting intimal lipid accumulation.

Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

Life Sciences ◽  
2000 ◽  
Vol 67 (24) ◽  
pp. 2983-2989 ◽  
Author(s):  
Zhiyuan Li ◽  
Yasuharu Niwa ◽  
Sadaichi Sakamoto ◽  
Masayuki Shono ◽  
Xiu Chen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxide Synthase ◽  
Porcine Endothelial Cells ◽  
Inducible Nitric Oxide
Sign in / Sign up

Export Citation Format

Share Document