scholarly journals IDENTIFICATION AND CHARACTERIZATION OF BRANCHED‐CHAIN AMINO ACID (BCAA) METABOLON PROTEINS: ROLE OF GLUTAMATE DEHYDROGENASE

2008 ◽  
Vol 22 (S1) ◽  
Author(s):  
Mohammad Mainul Islam ◽  
Manisha Nautiyal ◽  
James Mobley ◽  
Susan Hutson
Keyword(s):  
Amino Acid ◽  
Glutamate Dehydrogenase ◽  
Branched Chain Amino Acid ◽  
Branched Chain ◽  
Identification And Characterization

The role of branched chain amino acid and tryptophan metabolism in rat’s behavioral diversity: Intertwined peripheral and brain effects

2015 ◽  
Vol 25 (10) ◽  
pp. 1695-1705 ◽  
Author(s):  
Eyal Asor ◽  
Shiri Stempler ◽  
Avi Avital ◽  
Ehud Klein ◽  
Eytan Ruppin ◽  
...  
Keyword(s):  
Amino Acid ◽  
Tryptophan Metabolism ◽  
Behavioral Diversity ◽  
Branched Chain Amino Acid ◽  
Branched Chain

scholarly journals Transcriptional hallmarks of cancer cell lines reveal an emerging role of branched chain amino acid catabolism

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Ieva Antanavičiūtė ◽  
Valeryia Mikalayeva ◽  
Ieva Ceslevičienė ◽  
Gintarė Milašiūtė ◽  
Vytenis Arvydas Skeberdis ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Amino Acid Catabolism ◽  
Hallmarks Of Cancer ◽  
Branched Chain Amino Acid ◽  
Branched Chain

scholarly journals Branched chain amino acid aminotransferase 2 Regulates Ferroptotic Cell Death in Cancer Cells

2020 ◽  
Author(s):  
Kang Wang ◽  
Zhengyang Zhang ◽  
Tsai Hsiang-i ◽  
Yanfang Liu ◽  
Ming Wang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Cancer Cells ◽  
Therapeutic Strategy ◽  
Ectopic Expression ◽  
Branched Chain Amino Acid ◽  
Pancreatic Cancer Cells ◽  
Branched Chain

AbstractFerroptosis has been implicated as a tumor-suppressor function for cancer therapy. Recently the sensitivity to ferroptosis was tightly linked to numerous biological processes, including metabolism of amino acid. Here, using a high-throughput CRISPR/Cas9 based genetic screen in HepG2 cells to search for metabolic proteins inhibiting ferroptosis, we identified branched chain amino acid aminotransferase 2 (BCAT2) as a novel suppressor of ferroptosis. Mechanistically, ferroptosis inducers (erastin, sorafenib and sulfasalazine) activated AMPK/SREBP1 signaling pathway through ferritinophagy, which in turn inhibited BCAT2 transcription. We further confirmed that BCAT2 mediating the metabolism of sulfur amino acid, regulated intracellular glutamate level, whose activation by ectopic expression specifically antagonize system Xc– inhibition and protected liver and pancreatic cancer cells from ferroptosis in vitro and in vivo. Finally, our results demonstrate the synergistic effect of sorafenib and sulfasalazine in downregulating BCAT2 expression and dictating ferroptotic death, where BCAT2 can also be used to predict the responsiveness of cancer cells to ferroptosis-inducing therapies. Collectively, these findings identify a novel role of BCAT2 in ferroptosis, suggesting a potential therapeutic strategy for overcoming sorafenib resistance.

Sign in / Sign up

Export Citation Format

Share Document