scholarly journals LPS‐Induced Hyper‐permeability in Human Lung Microvascular Endothelial Cells (HLMVEC) Involves the Nitration‐Mediated Activation of RhoA

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Ruslan Rafikov ◽  
Saurabh Aggarwal ◽  
Yali Hou ◽  
Connie Snead ◽  
John Catravas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Increased ADMA/protein methylation modulates nitric oxide generation in hypoxic human lung microvascular endothelial cells

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Zhaosheng Han ◽  
Arthur J. Pope ◽  
Arturo J. Cardounel ◽  
Leif D Nelin ◽  
Louis G. Chicione
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Protein Methylation ◽  
Microvascular Endothelial

scholarly journals MicroRNA-126-3p Inhibits Angiogenic Function of Human Lung Microvascular Endothelial Cells via LAT1 (L-Type Amino Acid Transporter 1)-Mediated mTOR (Mammalian Target of Rapamycin) Signaling

2020 ◽  
Vol 40 (5) ◽  
pp. 1195-1206 ◽  
Author(s):  
Danting Cao ◽  
Andrew M. Mikosz ◽  
Alexandra J. Ringsby ◽  
Kelsey C. Anderson ◽  
Erica L. Beatman ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Amino Acid ◽  
Cell Apoptosis ◽  
Human Lung ◽  
Mammalian Target Of Rapamycin ◽  
Tube Formation ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

Objective: MicroRNA-126-3p (miR-126) is required for angiogenesis during organismal development or the repair of injured arterial vasculature. The role of miR-126 in lung microvascular endothelial cells, which are essential for gas exchange and for lung injury repair and regeneration, remains poorly understood. Considering the significant heterogeneity of endothelial cells from different vascular beds, we aimed to determine the role of miR-126 in regulating lung microvascular endothelial cell function and to elucidate its downstream signaling pathways. Approach and Results: Overexpression and knockdown of miR-126 in primary human lung microvascular endothelial cells (HLMVEC) were achieved via transfections of miR-126 mimics and antisense inhibitors. Increasing miR-126 levels in HLMVEC reduced cell proliferation, weakened tube formation, and increased cell apoptosis, whereas decreased miR-126 levels stimulated cell proliferation and tube formation. Whole-genome RNA sequencing revealed that miR-126 was associated with an antiangiogenic and proapoptotic transcriptomic profile. Using validation assays and knockdown approaches, we identified that the effect of miR-126 on HLMVEC angiogenesis was mediated by the LAT1 (L-type amino acid transporter 1), via regulation of mTOR (mammalian target of rapamycin) signaling. Furthermore, downregulation of miR-126 in HLMVEC inhibited cell apoptosis and improved endothelial tube formation during exposure to environmental insults such as cigarette smoke. Conclusions: miR-126 inhibits HLMVEC angiogenic function by targeting the LAT1-mTOR signaling axis, suggesting that miR-126 inhibition may be useful for conditions associated with microvascular loss, whereas miR-126 augmentation may help control unwanted microvascular angiogenesis.

scholarly journals Harvesting, identification and barrier function of human lung microvascular endothelial cells

2010 ◽  
Vol 52 (5-6) ◽  
pp. 175-181 ◽  
Author(s):  
John D. Catravas ◽  
Connie Snead ◽  
Christiana Dimitropoulou ◽  
Albert S.Y. Chang ◽  
Rudolf Lucas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Barrier Function ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Heterogeneous elastic response of human lung microvascular endothelial cells to barrier modulating stimuli

2013 ◽  
Vol 9 (7) ◽  
pp. 875-884 ◽  
Author(s):  
Fernando Terán Arce ◽  
Brian Meckes ◽  
Sara M. Camp ◽  
Joe G.N. Garcia ◽  
Steven M. Dudek ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Elastic Response ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

IL18 induces p38 MAP kinase activation and adhesion capacities in BMPRII knocked down human lung microvascular endothelial cells

2016 ◽  
Author(s):  
Leanda Vengethasamy ◽  
Rik Gijsbers ◽  
Annelies Michiels ◽  
Birger Tielemans ◽  
Allard Wagenaar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Map Kinase ◽  
Human Lung ◽  
P38 Map Kinase ◽  
Microvascular Endothelial Cells ◽  
Kinase Activation ◽  
Microvascular Endothelial

Effect of BMPRII on endothelial function in human lung microvascular endothelial cells

2018 ◽  
Author(s):  
Birger Tielemans ◽  
Rik Gijsbers ◽  
Annelies Michiels ◽  
Allard Wagenaar ◽  
Ricard Farre Marti ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Function ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial
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