scholarly journals Increased ADMA/protein methylation modulates nitric oxide generation in hypoxic human lung microvascular endothelial cells

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Zhaosheng Han ◽  
Arthur J. Pope ◽  
Arturo J. Cardounel ◽  
Leif D Nelin ◽  
Louis G. Chicione
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Protein Methylation ◽  
Microvascular Endothelial

scholarly journals LPS‐Induced Hyper‐permeability in Human Lung Microvascular Endothelial Cells (HLMVEC) Involves the Nitration‐Mediated Activation of RhoA

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Ruslan Rafikov ◽  
Saurabh Aggarwal ◽  
Yali Hou ◽  
Connie Snead ◽  
John Catravas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals MicroRNA-126-3p Inhibits Angiogenic Function of Human Lung Microvascular Endothelial Cells via LAT1 (L-Type Amino Acid Transporter 1)-Mediated mTOR (Mammalian Target of Rapamycin) Signaling

2020 ◽  
Vol 40 (5) ◽  
pp. 1195-1206 ◽  
Author(s):  
Danting Cao ◽  
Andrew M. Mikosz ◽  
Alexandra J. Ringsby ◽  
Kelsey C. Anderson ◽  
Erica L. Beatman ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Amino Acid ◽  
Cell Apoptosis ◽  
Human Lung ◽  
Mammalian Target Of Rapamycin ◽  
Tube Formation ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

Objective: MicroRNA-126-3p (miR-126) is required for angiogenesis during organismal development or the repair of injured arterial vasculature. The role of miR-126 in lung microvascular endothelial cells, which are essential for gas exchange and for lung injury repair and regeneration, remains poorly understood. Considering the significant heterogeneity of endothelial cells from different vascular beds, we aimed to determine the role of miR-126 in regulating lung microvascular endothelial cell function and to elucidate its downstream signaling pathways. Approach and Results: Overexpression and knockdown of miR-126 in primary human lung microvascular endothelial cells (HLMVEC) were achieved via transfections of miR-126 mimics and antisense inhibitors. Increasing miR-126 levels in HLMVEC reduced cell proliferation, weakened tube formation, and increased cell apoptosis, whereas decreased miR-126 levels stimulated cell proliferation and tube formation. Whole-genome RNA sequencing revealed that miR-126 was associated with an antiangiogenic and proapoptotic transcriptomic profile. Using validation assays and knockdown approaches, we identified that the effect of miR-126 on HLMVEC angiogenesis was mediated by the LAT1 (L-type amino acid transporter 1), via regulation of mTOR (mammalian target of rapamycin) signaling. Furthermore, downregulation of miR-126 in HLMVEC inhibited cell apoptosis and improved endothelial tube formation during exposure to environmental insults such as cigarette smoke. Conclusions: miR-126 inhibits HLMVEC angiogenic function by targeting the LAT1-mTOR signaling axis, suggesting that miR-126 inhibition may be useful for conditions associated with microvascular loss, whereas miR-126 augmentation may help control unwanted microvascular angiogenesis.

scholarly journals Lipoic acid antagonizes paraquat-induced vascular endothelial dysfunction by suppressing mitochondrial reactive oxidative stress

2019 ◽  
Vol 8 (6) ◽  
pp. 918-927 ◽  
Author(s):  
Li Pang ◽  
Ping Deng ◽  
Yi-dan Liang ◽  
Jing-yu Qian ◽  
Li-Chuan Wu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Endothelial Cells ◽  
Endothelial Dysfunction ◽  
Lipoic Acid ◽  
Microvascular Endothelial Cells ◽  
Ros Generation ◽  
Protective Effects ◽  
Migration Ability ◽  
Microvascular Endothelial

Abstract Paraquat (PQ) is a widely used herbicide in the agricultural field. The lack of an effective antidote is the significant cause of high mortality in PQ poisoning. Here, we investigate the antagonistic effects of alpha lipoic acid (α-LA), a naturally existing antioxidant, on PQ toxicity in human microvascular endothelial cells (HMEC-1). All the doses of 250, 500 and 1000 μM α-LA significantly inhibited 1000 μM PQ-induced cytotoxicity in HMEC-1 cells. α-LA pretreatment remarkably diminished the damage to cell migration ability, recovered the declined levels of the vasodilator factor nitric oxide (NO), elevated the expression level of endothelial nitric oxide synthases (eNOS), and inhibited the upregulated expression of vasoconstrictor factor endothelin-1 (ET-1). Moreover, α-LA pretreatment inhibited reactive oxygen species (ROS) generation, suppressed the damage to the mitochondrial membrane potential (ΔΨm) and mitigated the inhibition of adenosine triphosphate (ATP) production in HMEC-1 cells. These results suggested that α-LA could alleviate PQ-induced endothelial dysfunction by suppressing oxidative stress. In summary, our present study provides novel insight into the protective effects and pharmacological potential of α-LA against PQ toxicity in microvascular endothelial cells.

Differential expression of nitric oxide by dermal microvascular endothelial cells from patients with scleroderma

2000 ◽  
Vol 5 (3) ◽  
pp. 147-158 ◽  
Author(s):  
L.I. Romero ◽  
D-N. Zhang ◽  
J.P. Cooke ◽  
H-K. V Ho ◽  
E. Avalos ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Differential Expression ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial

scholarly journals Nitric oxide signaling in adipose triglyceride lipase-deficient microvascular endothelial cells

2012 ◽  
Vol 13 (Suppl 1) ◽  
pp. A22
Author(s):  
Marion Mussbacher ◽  
Sarah Winkler ◽  
Günter Hämmerle ◽  
Rudolf Zechner ◽  
Bernd Mayer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Microvascular Endothelial Cells ◽  
Adipose Triglyceride Lipase ◽  
Nitric Oxide Signaling ◽  
Triglyceride Lipase ◽  
Microvascular Endothelial

scholarly journals Harvesting, identification and barrier function of human lung microvascular endothelial cells

2010 ◽  
Vol 52 (5-6) ◽  
pp. 175-181 ◽  
Author(s):  
John D. Catravas ◽  
Connie Snead ◽  
Christiana Dimitropoulou ◽  
Albert S.Y. Chang ◽  
Rudolf Lucas ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Barrier Function ◽  
Human Lung ◽  
Microvascular Endothelial Cells ◽  
Microvascular Endothelial
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