Background:
By considering [6]-gingerol as an important polyphenol, ginger has been identified to be related to
cancer.
Objective:
In this study, the evaluation of ginger extract on the expressions of four genes from different categories involved
in breast cancer process in Balb/C mouse after 14 days was the main objective.
Methods:
In term of this research, ethanol extraction methods for extracting ginger rhizomes with the goal of increasing
[6]-gingerol were used. In this regard, MTT test was accomplished for whole ginger extract to confirm its effect on 4T1
breast cancer cell line (murine mammary carcinoma cell). Afterward, the transplanted breast cancer Balb/C mouse was used
to be treated by whole ginger extract (0.02 g/kg of mouse weight). The expressions of STAT3, β-catenin, P53, APC, genes
were evaluated, after 14-day treatment.
Results:
The results showed that 70% ethanol and microwave extraction method can be considered as the best method for
extracting ginger extract. The results of the expression of β-catenin has decreased in blood and liver of the treated group.
Moreover, the expression of STAT3 oncogenes has significantly decreased in the liver tissue. In addition, the expressions of
APC suppressor genes have increased in Blood and liver.
Conclusion:
In conclusion, treatment by 0.02 g/kg of mouse weight of ginger extract after 14 days increased the expressions
of APC as suppressor genes and β-catenin oncogene in Blood and liver of the breast cancer group.
Neuropeptide Y Y5‐receptor activation stimulates proliferation in the 4T1 breast cancer cell line
