Chromosomal mapping and zygosity check PCR of transgenes in a GFP mouse line (C57BL/6‐Tg(CAG‐EGFP)C14‐Y01‐FM131Osb) determined by genomic walking (1138.13)

Stingless bees of the genus Melipona are subdivided into 4 subgenera called Eomelipona, Melikerria, Melipona sensu stricto, and Michmelia according to species morphology. Cytogenetically, the species of the genus Melipona show variation in the amount and distribution of heterochromatin along their chromosomes and can be separated into 2 groups: the first with low content of heterochromatin and the second with high content of heterochromatin. These heterochromatin patterns and the number of chromosomes are characteristics exclusive to Melipona karyotypes that distinguish them from the other genera of the Meliponini. To better understand the karyotype organization in Melipona and the relationship among the subgenera, we mapped repetitive sequences and analyzed previously reported cytogenetic data with the aim to identify cytogenetic markers to be used for investigating the phylogenetic relationships and chromosome evolution in the genus. In general, Melipona species have 2n = 18 chromosomes, and the species of each subgenus share the same characteristics in relation to heterochromatin regions, DAPI/CMA3 fluorophores, and the number and distribution of 18S rDNA sites. Microsatellites were observed only in euchromatin regions, whereas the (TTAGG)6 repeats were found at telomeric sites in both groups. Our data indicate that in addition to the chromosome number, the karyotypes in Melipona could be separated into 2 groups that are characterized by conserved cytogenetic features and patterns that generally are shared by species within each subgenus, which may reflect evolutionary constraints. Our results agree with the morphological separation of the Melipona into 4 subgenera, suggesting that they must be independent evolutionary lineages.

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Cloning and chromosomal mapping of the human nonfunctional gene for L-gulono-gamma-lactone oxidase, the enzyme for L-ascorbic acid biosynthesis missing in man.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(17)36884-9 ◽

1994 ◽

Vol 269 (18) ◽

pp. 13685-13688

Author(s):

M. Nishikimi ◽

R. Fukuyama ◽

S. Minoshima ◽

N. Shimizu ◽

K. Yagi

Keyword(s):

Ascorbic Acid ◽

Chromosomal Mapping ◽

Acid Biosynthesis ◽

Ascorbic Acid Biosynthesis

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A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals

Function ◽

10.1093/function/zqab012 ◽

2021 ◽

Vol 2 (3) ◽

Cited By ~ 1

Author(s):

Nelly Redolfi ◽

Elisa Greotti ◽

Giulia Zanetti ◽

Tino Hochepied ◽

Cristina Fasolato ◽

...

Keyword(s):

Transgenic Mouse ◽

Fluorescent Protein ◽

Ex Vivo ◽

Brain Slices ◽

Resonance Energy ◽

Mouse Line ◽

Isolated Cells ◽

Genomic Locus ◽

Transgenic Mouse Line

AbstractMitochondria play a key role in cellular calcium (Ca2+) homeostasis. Dysfunction in the organelle Ca2+ handling appears to be involved in several pathological conditions, ranging from neurodegenerative diseases, cardiac failure and malignant transformation. In the past years, several targeted green fluorescent protein (GFP)-based genetically encoded Ca2+ indicators (GECIs) have been developed to study Ca2+ dynamics inside mitochondria of living cells. Surprisingly, while there is a number of transgenic mice expressing different types of cytosolic GECIs, few examples are available expressing mitochondria-localized GECIs, and none of them exhibits adequate spatial resolution. Here we report the generation and characterization of a transgenic mouse line (hereafter called mt-Cam) for the controlled expression of a mitochondria-targeted, Förster resonance energy transfer (FRET)-based Cameleon, 4mtD3cpv. To achieve this goal, we engineered the mouse ROSA26 genomic locus by inserting the optimized sequence of 4mtD3cpv, preceded by a loxP-STOP-loxP sequence. The probe can be readily expressed in a tissue-specific manner upon Cre recombinase-mediated excision, obtainable with a single cross. Upon ubiquitous Cre expression, the Cameleon is specifically localized in the mitochondrial matrix of cells in all the organs and tissues analyzed, from embryos to aged animals. Ca2+ imaging experiments performed in vitro and ex vivo in brain slices confirmed the functionality of the probe in isolated cells and live tissues. This new transgenic mouse line allows the study of mitochondrial Ca2+ dynamics in different tissues with no invasive intervention (such as viral infection or electroporation), potentially allowing simple calibration of the fluorescent signals in terms of mitochondrial Ca2+ concentration ([Ca2+]).

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A Novel Conserved Cochlear Gene, OTOR: Identification, Expression Analysis, and Chromosomal Mapping

Genomics ◽

10.1006/geno.2000.6224 ◽

2000 ◽

Vol 66 (3) ◽

pp. 242-248 ◽

Cited By ~ 29

Author(s):

Nahid G. Robertson ◽

Stefan Heller ◽

Jason S. Lin ◽

Barbara L. Resendes ◽

Stanislawa Weremowicz ◽

...

Keyword(s):

Expression Analysis ◽

Chromosomal Mapping

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Tissue-Specific Expression, Developmental Regulation, and Chromosomal Mapping of the Lecithin: Cholesterol Acyltransferase Gene

Journal of Biological Chemistry ◽

10.1016/s0021-9258(20)88222-2 ◽

1989 ◽

Vol 264 (36) ◽

pp. 21573-21581 ◽

Cited By ~ 1

Author(s):

C H Warden ◽

C A Langner ◽

J I Gordon ◽

B A Taylor ◽

J W McLean ◽

...

Keyword(s):

Developmental Regulation ◽

Cholesterol Acyltransferase ◽

Chromosomal Mapping ◽

Specific Expression ◽

Tissue Specific ◽

Tissue Specific Expression ◽

Lecithin Cholesterol Acyltransferase

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Sim1-expressing cells illuminate the origin and course of migration of the nucleus of the lateral olfactory tract in the mouse amygdala

Brain Structure and Function ◽

10.1007/s00429-020-02197-1 ◽

2021 ◽

Vol 226 (2) ◽

pp. 519-562 ◽

Cited By ~ 1

Author(s):

Elena Garcia-Calero ◽

Lara López-González ◽

Margaret Martínez-de-la-Torre ◽

Chen-Ming Fan ◽

Luis Puelles

Keyword(s):

Experimental Data ◽

Mouse Line ◽

Lateral Olfactory Tract ◽

Migration Stream ◽

Olfactory Tract ◽

Basolateral Nucleus ◽

Gene Functions ◽

Layer 2 ◽

Embryonic Origin ◽

Olfactory Input

AbstractWe focus this report on the nucleus of the lateral olfactory tract (NLOT), a superficial amygdalar nucleus receiving olfactory input. Mixed with its Tbr1-expressing layer 2 pyramidal cell population (NLOT2), there are Sim1-expressing cells whose embryonic origin and mode of arrival remain unclear. We examined this population with Sim1-ISH and a Sim1-tauLacZ mouse line. An alar hypothalamic origin is apparent at the paraventricular area, which expresses Sim1 precociously. This progenitor area shows at E10.5 a Sim1-expressing dorsal prolongation that crosses the telencephalic stalk and follows the terminal sulcus, reaching the caudomedial end of the pallial amygdala. We conceive this Sim1-expressing hypothalamo-amygdalar corridor (HyA) as an evaginated part of the hypothalamic paraventricular area, which participates in the production of Sim1-expressing cells. From E13.5 onwards, Sim1-expressing cells migrated via the HyA penetrate the posterior pallial amygdalar radial unit and associate therein to the incipient Tbr1-expressing migration stream which swings medially past the amygdalar anterior basolateral nucleus (E15.5), crosses the pallio-subpallial boundary (E16.5), and forms the NLOT2 within the anterior amygdala by E17.5. We conclude that the Tbr1-expressing NLOT2 cells arise strictly within the posterior pallial amygdalar unit, involving a variety of required gene functions we discuss. Our results are consistent with the experimental data on NLOT2 origin reported by Remedios et al. (Nat Neurosci 10:1141–1150, 2007), but we disagree on their implication in this process of the dorsal pallium, observed to be distant from the amygdala.

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