scholarly journals Novel role of sorting nexin 5 in renal D 1 dopamine receptor trafficking and function: implications for hypertension

2012 ◽  
Vol 27 (5) ◽  
pp. 1808-1819 ◽  
Author(s):  
Van Anthony M. Villar ◽  
Ines Armando ◽  
Hironobu Sanada ◽  
Lauren C. Frazer ◽  
Christen M. Russo ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
Receptor Trafficking ◽  
Sorting Nexin ◽  
And Function ◽  
Sorting Nexin 5

scholarly journals Conformational effects of Lys191 in the human GnRH receptor: mutagenesis and molecular dynamics simulations studies

2009 ◽  
Vol 201 (2) ◽  
pp. 297-307 ◽  
Author(s):  
Eduardo Jardón-Valadez ◽  
Arturo Aguilar-Rojas ◽  
Guadalupe Maya-Núñez ◽  
Alfredo Leaños-Miranda ◽  
Ángel Piñeiro ◽  
...  
Keyword(s):  
Molecular Dynamics ◽  
Molecular Dynamics Simulations ◽  
Dynamic Behavior ◽  
Gnrh Receptor ◽  
Receptor Trafficking ◽  
Wild Type ◽  
Conformational Effects ◽  
Dynamics Simulations ◽  
And Function

In the present study, we analyzed the role of Lys191 on function, structure, and dynamic behavior of the human GnRH receptor (hGnRHR) and the formation of the Cys14–Cys200 bridge, which is essential for receptor trafficking to the plasma membrane. Several mutants were studied; mutants lacked either the Cys14–Cys200 bridge, Lys191 or both. The markedly reduced expression and function of a Cys14Ser mutant lacking the 14–200 bridge, was nearly restored to wild-type/ΔLys191 levels upon deletion of Lys191. Lys191 removal resulted in changes in the dynamic behavior of the mutants as disclosed by molecular dynamics simulations: the distance between the sulfur- (or oxygen-) sulfur groups of Cys (or Ser)14 and Cys200 was shorter and more constant, and the conformation of the NH2-terminus and the exoloop 2 exhibited fewer fluctuations than when Lys191 was present. These data provide novel information on the role of Lys191 in defining an optimal configuration for the hGnRHR intracellular trafficking and function.

scholarly journals Role of dysbindin in dopamine receptor trafficking and cortical GABA function

2009 ◽  
Vol 106 (46) ◽  
pp. 19593-19598 ◽  
Author(s):  
Y. Ji ◽  
F. Yang ◽  
F. Papaleo ◽  
H.-X. Wang ◽  
W.-J. Gao ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
Receptor Trafficking ◽  
Gaba Function

scholarly journals Role of LRRK2 in the regulation of dopamine receptor trafficking

PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179082 ◽  
Author(s):  
Mauro Rassu ◽  
Maria Grazia Del Giudice ◽  
Simona Sanna ◽  
Jean Marc Taymans ◽  
Michele Morari ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
Receptor Trafficking

scholarly journals Clathrin Isoform CHC22, a Component of Neuromuscular and Myotendinous Junctions, Binds Sorting Nexin 5 and Has Increased Expression during Myogenesis and Muscle Regeneration

2004 ◽  
Vol 15 (7) ◽  
pp. 3181-3195 ◽  
Author(s):  
Mhairi C. Towler ◽  
Paul A. Gleeson ◽  
Sachiko Hoshino ◽  
Paavo Rahkila ◽  
Venus Manalo ◽  
...  
Keyword(s):  
Time Course ◽  
Muscle Development ◽  
Coiled Coil ◽  
Myoblast Differentiation ◽  
Sorting Nexin ◽  
Binding Data ◽  
Differential Binding ◽  
And Function ◽  
Myotendinous Junctions ◽  
Sorting Nexin 5

The muscle isoform of clathrin heavy chain, CHC22, has 85% sequence identity to the ubiquitously expressed CHC17, yet its expression pattern and function appear to be distinct from those of well-characterized clathrin-coated vesicles. In mature muscle CHC22 is preferentially concentrated at neuromuscular and myotendinous junctions, suggesting a role at sarcolemmal contacts with extracellular matrix. During myoblast differentiation, CHC22 expression is increased, initially localized with desmin and nestin and then preferentially segregated to the poles of fused myoblasts. CHC22 expression is also increased in regenerating muscle fibers with the same time course as embryonic myosin, indicating a role in muscle repair. CHC22 binds to sorting nexin 5 through a coiled-coil domain present in both partners, which is absent in CHC17 and coincides with the region on CHC17 that binds the regulatory light-chain subunit. These differential binding data suggest a mechanism for the distinct functions of CHC22 relative to CHC17 in membrane traffic during muscle development, repair, and at neuromuscular and myotendinous junctions.

Scanning electron microscopic study of collagen fibrillogenesis and extracellular compartmentalization in the chick embryo tendon

1986 ◽  
Vol 44 ◽  
pp. 208-209
Author(s):  
Grace C.H. Yang
Keyword(s):  
Chick Embryo ◽  
Extracellular Space ◽  
Fibroblast Cell ◽  
Collagen Fibrils ◽  
Electron Microscopic ◽  
Voltage Electron ◽  
Scanning Electron Microscopic Study ◽  
Microscopic Studies ◽  
And Function

The size and organization of collagen fibrils in the extracellular matrix is an important determinant of tissue structure and function. The synthesis and deposition of collagen involves multiple steps which begin within the cell and continue in the extracellular space. High-voltage electron microscopic studies of the chick embryo cornea and tendon suggested that the extracellular space is compartmentalized by the fibroblasts for the regulation of collagen fibril, bundle, and tissue specific macroaggregate formation. The purpose of this study is to gather direct evidence regarding the association of the fibroblast cell surface with newly formed collagen fibrils, and to define the role of the fibroblast in the control and the precise positioning of collagen fibrils, bundles, and macroaggregates during chick tendon development.

Role of the Cilia Membrane in Control of Microtubule Sliding

1980 ◽  
Vol 38 ◽  
pp. 612-615
Author(s):  
Edna S. Kaneshiro
Keyword(s):  
Control Mechanism ◽  
Beat Frequency ◽  
Surface Membrane ◽  
Ciliary Membrane ◽  
Ciliary Beating ◽  
Ciliary Reversal ◽  
Voltage Dependent ◽  
Reversal Mechanism ◽  
And Function

It is currently believed that ciliary beating results from microtubule sliding which is restricted in regions to cause bending. Cilia beat can be modified to bring about changes in beat frequency, cessation of beat and reversal in beat direction. In ciliated protozoans these modifications which determine swimming behavior have been shown to be related to intracellular (intraciliary) Ca2+ concentrations. The Ca2+ levels are in turn governed by the surface ciliary membrane which exhibits increased Ca2+ conductance (permeability) in response to depolarization. Mutants with altered behaviors have been isolated. Pawn mutants fail to exhibit reversal of the effective stroke of ciliary beat and therefore cannot swim backward. They lack the increased inward Ca2+ current in response to depolarizing stimuli. Both normal and pawn Paramecium made leaky to Ca2+ by Triton extrac¬tion of the surface membrane exhibit backward swimming only in reactivating solutions containing greater than IO-6 M Ca2+ Thus in pawns the ciliary reversal mechanism itself is left operational and only the control mechanism at the membrane is affected. The topographic location of voltage-dependent Ca2+ channels has been identified as a component of the ciliary mem¬brane since the inward Ca2+ conductance response is eliminated by deciliation and the return of the response occurs during cilia regeneration. Since the ciliary membrane has been impli¬cated in the control of Ca2+ levels in the cilium and therefore is the site of at least one kind of control of microtubule sliding, we have focused our attention on understanding the structure and function of the membrane.

POPDC proteins and cardiac function

2019 ◽  
Vol 47 (5) ◽  
pp. 1393-1404 ◽  
Author(s):  
Thomas Brand
Keyword(s):  
Potential Role ◽  
Striated Muscle ◽  
Short Review ◽  
Effector Proteins ◽  
Muscle Disease ◽  
Disease Genes ◽  
Protein Protein Interaction ◽  
Interaction Partners ◽  
And Function

Abstract The Popeye domain-containing gene family encodes a novel class of cAMP effector proteins in striated muscle tissue. In this short review, we first introduce the protein family and discuss their structure and function with an emphasis on their role in cyclic AMP signalling. Another focus of this review is the recently discovered role of POPDC genes as striated muscle disease genes, which have been associated with cardiac arrhythmia and muscular dystrophy. The pathological phenotypes observed in patients will be compared with phenotypes present in null and knockin mutations in zebrafish and mouse. A number of protein–protein interaction partners have been discovered and the potential role of POPDC proteins to control the subcellular localization and function of these interacting proteins will be discussed. Finally, we outline several areas, where research is urgently needed.

Role of trace amine associated receptor 1 (TAAR1) in D2 dopamine receptor-related behavior and signaling

2012 ◽  
Author(s):  
S. Espinoza ◽  
F. Manago ◽  
M. Messa ◽  
T. D. Sotnikova ◽  
M. Caron ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
D2 Dopamine Receptor ◽  
Trace Amine

Role of Trace Amine Associated Receptor 1 (TAAR1) in D2 Dopamine receptor-related behavior and signaling

2012 ◽  
Author(s):  
S. Espinoza ◽  
F. Manago ◽  
M. Messa ◽  
T. D. Sotnikova ◽  
M. Caron ◽  
...  
Keyword(s):  
Dopamine Receptor ◽  
D2 Dopamine Receptor ◽  
Trace Amine
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