Testicular cells exhibit similar molecular responses to cigarette smoke condensate ex vivo and in vivo

2017 ◽  
Vol 32 (1) ◽  
pp. 63-72 ◽  
Author(s):  
Prabagaran Esakky ◽  
Deborah A. Hansen ◽  
Andrea M. Drury ◽  
Paul Felder ◽  
Andrew Cusumano ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Ex Vivo ◽  
Cigarette Smoke Condensate ◽  
Molecular Responses ◽  
Testicular Cells

Humic-like substances in cigarette smoke condensate and lung tissue of smokers

1994 ◽  
Vol 266 (4) ◽  
pp. L382-L388 ◽  
Author(s):  
A. J. Ghio ◽  
J. Stonehuerner ◽  
D. R. Quigley
Keyword(s):  
Free Radical ◽  
Cigarette Smoke ◽  
Lung Tissue ◽  
Cigarette Smoke Exposure ◽  
Cigarette Smoke Condensate ◽  
Free Radical Generation ◽  
Carboxylate Content ◽  
Radical Generation

Deposition of pigmented matter in the lower respiratory tract correlates with the extent of emphysema in smokers as well as with free radical generation and iron accumulation. Pulmonary emphysema is postulated to be mediated by free radical generation which is either directly or indirectly associated with cigarette smoke exposure. The hypothesis was tested that 1) incomplete combustion of tobacco yields humic-like substances (HLS) which 2) deposit in the lung as pigmented particulates, 3) complex iron cations in vitro and in vivo, and 4) have a capacity to catalyze oxidant formation. HLS, isolated by alkali extraction of cigarette smoke condensate (CSC) (Tobacco Health Research Institute, University of Kentucky), demonstrated a high carbon and low carboxylate content on elemental and functional group analyses, respectively, compared with values for HLS sequestered from soils. The HLS isolated from CSC had a capacity to complex iron in vitro and accumulated the metal in vivo after intratracheal instillation in an animal model. Both HLS and its iron complex generated free radicals, and some portion of this oxidant generation was metal dependent. Lung tissue collected at autopsy from smokers contained HLS with an infrared spectrum almost identical to that of the material isolated from CSC. Associations between particulate deposition, metal accumulation, and free radical generation suggest a possible role of HLS in the induction of lung disease following cigarette exposure.

In vitro and in vivo immunotoxic and immunomodulatory effects of nonsupplemented and selenium-supplemented cigarette smoke condensate

2004 ◽  
Vol 58 (2) ◽  
pp. 90-94 ◽  
Author(s):  
Pascale Nguyen Van Binh ◽  
Dong Zhou ◽  
Françoise Baudouin ◽  
Chantal Martin ◽  
Martine Radionoff ◽  
...  
Keyword(s):  
Cigarette Smoke ◽  
Cigarette Smoke Condensate ◽  
Immunomodulatory Effects

Modulation of the mutagenic activity of cigarette smoke, cigarette smoke condensate and benzo[a]pyrene in vitro and in vivo

Mutagenesis ◽  
1994 ◽  
Vol 9 (2) ◽  
pp. 107-112 ◽  
Author(s):  
Roumen Balansky ◽  
Zvetanka Mircheva ◽  
Penka Blagoeva
Keyword(s):  
Cigarette Smoke ◽  
Mutagenic Activity ◽  
Cigarette Smoke Condensate

scholarly journals Baseline in vivo, ex vivo and molecular responses of Plasmodium falciparum to artemether and lumefantrine in three endemic zones for malaria in Colombia

2017 ◽  
Vol 111 (2) ◽  
pp. 71-80 ◽  
Author(s):  
Samanda Aponte ◽  
Ángela Patricia Guerra ◽  
Catalina Álvarez-Larrotta ◽  
Sindy Durley Bernal ◽  
César Restrepo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Ex Vivo ◽  
Molecular Responses

scholarly journals Transcriptional Response of Mycobacterium tuberculosis to Cigarette Smoke Condensate

2021 ◽  
Vol 12 ◽  
Author(s):  
Danicke Willemse ◽  
Chivonne Moodley ◽  
Smriti Mehra ◽  
Deepak Kaushal
Keyword(s):  
Oxidative Stress ◽  
Mycobacterium Tuberculosis ◽  
Cigarette Smoke ◽  
Mutation Frequency ◽  
Transcriptional Response ◽  
Cigarette Smoke Condensate ◽  
Stress Survival ◽  
The Impact

Smoking is known to be an added risk factor for tuberculosis (TB), with nearly a quarter of the TB cases attributed to cigarette smokers in the 22 countries with the highest TB burden. Many studies have indicated a link between risk of active TB and cigarette smoke. Smoking is also known to significantly decrease TB cure and treatment completion rate and increase mortality rates. Cigarette smoke contains thousands of volatile compounds including carcinogens, toxins, reactive solids, and oxidants in both particulate and gaseous phase. Yet, to date, limited studies have analyzed the impact of cigarette smoke components on Mycobacterium tuberculosis (Mtb), the causative agent of TB. Here we report the impact of cigarette smoke condensate (CSC) on survival, mutation frequency, and gene expression of Mtb in vitro. We show that exposure of virulent Mtb to cigarette smoke increases the mutation frequency of the pathogen and strongly induces the expression of the regulon controlled by SigH—a global transcriptional regulator of oxidative stress. SigH has previously been shown to be required for Mtb to respond to oxidative stress, survival, and granuloma formation in vivo. A high-SigH expression phenotype is known to be associated with greater virulence of Mtb. In patients with pulmonary TB who smoke, these changes may therefore play an important, yet unexplored, role in the treatment efficacy by potentially enhancing the virulence of tubercle bacilli.

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