The lysosomal membrane protein Sidt2 is a vital regulator of mitochondrial quality control in skeletal muscle

2021 ◽  
Vol 35 (4) ◽  
Author(s):  
Lizhuo Wang ◽  
Cui Yu ◽  
Wenjun Pei ◽  
Mengya Geng ◽  
Yao Zhang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Mitochondrial Quality Control ◽  
Lysosomal Membrane Protein

Short term intensified training temporarily impairs mitochondrial respiratory capacity in elite endurance athletes

2021 ◽  
Author(s):  
Daniele A. Cardinale ◽  
Kasper D. Gejl ◽  
Kristine Grøsfjeld Petersen ◽  
Joachim Nielsen ◽  
Niels Ørtenblad ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Oxidative Capacity ◽  
Endurance Athletes ◽  
Training Period ◽  
Moderate Intensity ◽  
Mitochondrial Quality Control ◽  
Quality Control Program ◽  
Mitochondrial Oxidative Capacity ◽  
Mitochondrial Respiratory

Aim: The maintenance of healthy and functional mitochondria is the result of a complex mitochondrial turnover and herein quality-control program which includes both mitochondrial biogenesis and autophagy of mitochondria. The aim of this study was to examine the effect of an intensified training load on skeletal muscle mitochondrial quality control in relation to changes in mitochondrial oxidative capacity, maximal oxygen consumption and performance in highly trained endurance athletes. Methods: 27 elite endurance athletes performed high intensity interval exercise followed by moderate intensity continuous exercise 3 days per week for 4 weeks in addition to their usual volume of training. Mitochondrial oxidative capacity, abundance of mitochondrial proteins, markers of autophagy and antioxidant capacity of skeletal muscle were assessed in skeletal muscle biopsies before and after the intensified training period. Results: The intensified training period increased several autophagy markers suggesting an increased turnover of mitochondrial and cytosolic proteins. In permeabilized muscle fibers, mitochondrial respiration was ~20 % lower after training although some markers of mitochondrial density increased by 5-50%, indicative of a reduced mitochondrial quality by the intensified training intervention. The antioxidative proteins UCP3, ANT1, and SOD2 were increased after training, whereas we found an inactivation of aconitase. In agreement with the lower aconitase activity, the amount of mitochondrial LON protease that selectively degrades oxidized aconitase, was doubled. Conclusion: Together, this suggests that mitochondrial respiratory function is impaired during the initial recovery from a period of intensified endurance training while mitochondrial quality control is slightly activated in highly trained skeletal muscle.

scholarly journals The lysosomal membrane protein LAMP2A promotes autophagic flux and prevents SNCA-induced Parkinson disease-like symptoms in the Drosophila brain

Autophagy ◽  
2018 ◽  
Vol 14 (11) ◽  
pp. 1898-1910 ◽  
Author(s):  
Abdul-Raouf Issa ◽  
Jun Sun ◽  
Céline Petitgas ◽  
Ana Mesquita ◽  
Amina Dulac ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Parkinson Disease ◽  
Lysosomal Membrane ◽  
Autophagic Flux ◽  
Drosophila Brain ◽  
Lysosomal Membrane Protein

Expression of the lysosomal membrane protein LMBD1 is essential for the initiation of gastrulation

2017 ◽  
Vol 120 (1-2) ◽  
pp. S117
Author(s):  
Frank Rutsch ◽  
Petra Pennekamp ◽  
Yvonne Nitschke ◽  
Chrishanthi Lowe ◽  
Boris Skryabin ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein

scholarly journals Functional characterization of the lysosomal membrane protein TMEM192 in mice

Oncotarget ◽  
2017 ◽  
Vol 8 (27) ◽  
pp. 43635-43652 ◽  
Author(s):  
Thuy Linh Nguyen ◽  
Janna Schneppenheim ◽  
Sönke Rudnik ◽  
Renate Lüllmann-Rauch ◽  
Christian Bernreuther ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Functional Characterization ◽  
Lysosomal Membrane ◽  
Lysosomal Membrane Protein

scholarly journals OR-043 UPRmt and mitophagy are selectively activated depending on muscle fiber types in insulin resistant rats

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Can Li ◽  
Nan Li ◽  
Yong Zhang
Keyword(s):  
Insulin Resistance ◽  
Skeletal Muscle ◽  
Quality Control ◽  
Muscle Fiber ◽  
Slow Muscle ◽  
Muscle Fiber Types ◽  
Fiber Types ◽  
Mitochondrial Quality Control ◽  
Insulin Resistant ◽  
Related Proteins

Objective To investigate how different skeletal muscle fiber types affect development of insulin resistance, and to explore the role of mitochondrial quality control system, especially mitochondrial unfolded protein response (UPRmt) and mitophagy, in response to metabolic stresses. Methods Male Wistar rats were randomly divided into 2 groups: fed with the normal diet for 8 weeks (Con), and fed with 45% high-fat diet for 8 weeks (IR). Fasting blood glucose (FBG), fasting insulin (FIN) and oral glucose tolerance test (OGTT) were used to identify insulin resistance model. Gastrocnemius (GC), soleus (SOL) and tibialis anterior (TA) muscle were isolated, and RT-qPCR was used to determine the expression of Myhc7, Myhc4. Oxygraph-2k was used to determine the mitochondrial State 3 (ST3), State 4(ST4) respiration and respiration control rate (RCR). JC-1 probe was used to measure mitochondrial membrane potential. Western Blot was used to determine the expressions of marker proteins of muscle fiber types (Myhc4, Myhc7), UPRmt related proteins (Hsp60, Hsp70) and mitophagy related proteins (Pink1, LC3). Results Compared with Con group, in IR group, FBG (7.1±1.27 vs. 5.4±0.43，p＜0.05), FIN (19.4±5.2 vs. 31.6±6.7，p＜0.05 ) and OGTT (area under the curve, about 31.7% increases, p＜0.05) were significantly higher. Myhc4 mRNA (relative fold about 55.6% increases) and protein expression (about 33.9% increases, p＜0.05) were significantly higher in GC. Myhc4 protein expression was significantly higher in GC (about 60.5% increases, p＜0.05). While Myhc7 mRNA expression (about 51.1% decreases, p＜0.05) was significantly lower in SOL. Compared with Con group, in IR group, mitochondrial RCR in SOL muscle was significantly lower (about 22.5% decreases, p＜0.05). Furthermore, the expression of HSP60 (about 36.7% increases，p＜0.05) and HSP70 (about 44.3% increases，p＜0.05) was significantly higher in TA muscle, while the expression of Parkin (about 18.8% decreases，p＜0.05) and the ratio between LC3 II/I (about 26.0% decreases，p＜0.05)expression in SOL muscle were significantly lower. Conclusions In this study, we found that the percentage of fast muscle fibers was elevated in IR skeletal muscle, which were supported by increased Myhc4 and decreased Myhc7 level. Impaired mitochondrial function was only observed in slow muscle as inhibition of mitochondrial respiration. As marker of UPRmt, HSP60/70 were specifically activated in fast muscle in IR, while mitophagy-related proteins were specifically increased in slow muscle. These results indicate that mitochondrial quality control systems are selectively activated to recover mitochondrial functions depending on muscle fiber types in insulin resistant rat.

The influence of aerobic exercise on mitochondrial quality control in skeletal muscle

2021 ◽  
Author(s):  
Ashleigh M. Philp ◽  
Nicholas J. Saner ◽  
Michael Lazarou ◽  
Ian G. Ganley ◽  
Andrew Philp
Keyword(s):  
Skeletal Muscle ◽  
Quality Control ◽  
Aerobic Exercise ◽  
Mitochondrial Quality Control

scholarly journals β2-Microglobulin Amyloid Fibrils Are Nanoparticles That Disrupt Lysosomal Membrane Protein Trafficking and Inhibit Protein Degradation by Lysosomes

2014 ◽  
Vol 289 (52) ◽  
pp. 35781-35794 ◽  
Author(s):  
Toral Jakhria ◽  
Andrew L. Hellewell ◽  
Morwenna Y. Porter ◽  
Matthew P. Jackson ◽  
Kevin W. Tipping ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Degradation ◽  
Protein Trafficking ◽  
Amyloid Fibrils ◽  
Lysosomal Membrane ◽  
Β2 Microglobulin ◽  
Membrane Protein Trafficking ◽  
Lysosomal Membrane Protein
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