Three Immunostaining Techniques for the Localization of Leukocyte Common Antigen in Formalin-Fixed, Paraffin-Embedded Dermatological Biopsy Specimens

The relationship between expression of the c-erbB-2 proto-oncogene and the biology of breast cancer has been investigated widely, most studies using immunohistochemistry in formalin-fixed, paraffin-embedded tissues. This technique is at best semiquantitative and there is a high degree of interstudy variability because of its subjective nature and poor methodological standardization. The relationship between the levels of expression and biology can be examined thoroughly only with an accurately quantitative technique. We have developed a radioimmunohistochemical assay to measure p185(erbB-2) in tissue biopsy specimens. The method involves incubating frozen sections with 125I-labeled monoclonal antibody, microautoradiograpy, and grain counting with image analysis. Sections of cell pellets with known c-erbB-2 levels are processed with each batch of samples as internal calibration standards. We have quantified c-erbB-2 expression in 60 breast carcinomas and compared the results with conventional immunohistochemistry. Radioimmunohistochemistry measured receptor levels throughout the range of expression in breast carcinomas, whereas conventional immunohistochemistry detected the protein only in the highest expressing tumors. The quantitative, objective data produced by radioimmunohistochemistry allow a more thorough evaluation of the relationship between c-erbB-2 expression and tumor biology. This technique may have applications in other fields where quantitative data is required and relevant monoclonal antibodies are available.

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Abstract A050: Validation of β-catenin as a tumor segmentation marker for delineating tumor from stromal tissues in quantitative multiplex immunofluorescence analysis of formalin-fixed, paraffin-embedded biopsy specimens

10.1158/1535-7163.targ-19-a050 ◽

2019 ◽

Author(s):

Tony Navas ◽

Robert J. Kinders ◽

Hala Makhlouf ◽

Scott M. Lawrence ◽

Rodrigo Chuaqui ◽

...

Keyword(s):

Tumor Segmentation ◽

Immunofluorescence Analysis ◽

Biopsy Specimens ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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A comparison of immunohistochemistry and mass spectrometry for determining the amyloid fibril protein from formalin-fixed biopsy tissue

Journal of Clinical Pathology ◽

10.1136/jclinpath-2014-202722 ◽

2015 ◽

Vol 68 (4) ◽

pp. 314-317 ◽

Cited By ~ 57

Author(s):

Janet A Gilbertson ◽

Jason D Theis ◽

Julie A Vrana ◽

Helen Lachmann ◽

Ashutosh Wechalekar ◽

...

Keyword(s):

Mass Spectrometry ◽

Amyloid Fibrils ◽

Accurate Identification ◽

Protein Precursor ◽

Biopsy Specimens ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Amyloid Fibril Protein ◽

The Uk ◽

Formalin Fixed

Amyloidosis is caused by deposition in tissues of abnormal protein in a characteristic fibrillar form. There are many types of amyloidosis, classified according to the soluble protein precursor from which the amyloid fibrils are derived. Accurate identification of amyloid type is critical in every case since therapy for systemic amyloidosis is type specific. In ∼20–25% cases, however, immunohistochemistry (IHC) fails to prove the amyloid type and further tests are required. Laser microdissection and mass spectrometry (LDMS) is a powerful tool for identifying proteins from formalin-fixed paraffin-embedded tissues. We undertook a blinded comparison of IHC, performed at the UK National Amyloidosis Centre, and LDMS, performed at the Mayo Clinic, in 142 consecutive biopsy specimens from 38 different tissue types. There was 100% concordance between positive IHC and LDMS, and the latter increased diagnostic accuracy from 76% to 94%. LDMS in expert hands is a valuable tool for amyloid diagnosis.

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Histocytochemistry of Glycoconjugates in Nasal Inverted Papilloma

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348949410300206 ◽

1994 ◽

Vol 103 (2) ◽

pp. 115-117 ◽

Cited By ~ 4

Author(s):

Hai Huang ◽

Desheng Jing ◽

Shuimiao Zhou ◽

Zhaoji Li ◽

Dalie Ma

Keyword(s):

Normal Mucosa ◽

Papillary Adenocarcinoma ◽

Inverted Papilloma ◽

Biopsy Specimens ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Glycoprotein Structure ◽

Biologic Characterization ◽

Formalin Fixed ◽

Inverted Papillomas

In order to investigate the changes in cellular distribution of the glycocalyces in nasal inverted papilloma, formalin-fixed, paraffin-embedded biopsy specimens of inverted papilloma were analyzed by the avidin-biotin-peroxidase complex technique for the demonstration of peanut agglutinin (PNA) receptors, concanavalin A ( Canavalia ensiformis agglutinin; ConA) receptors, carcinoembryonic antigen (CEA), and keratin, and compared with normal nasal mucosa, nasal polyps, and papillary adenocarcinoma. The inverted papillomas were positive for PNA and CEA, to the same degree as papillary adenocarcinoma. Their PNA binding was related to the degree of dysplasia. The ConA reaction was intermediate between that of normal mucosa and adenocarcinoma. The results suggest that the alteration of cellular glycoprotein structure in inverted papilloma is associated with its biologic characterization.

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