Immunohistochemical Analysis of Endometrial Adenocarcinoma for bcl-2 and p53 in Relation to Expression of Sex Steroid Receptor and Proliferative Activity

The immunoperoxidase stain for estrogen and progesterone receptor content in endometrial adenocarcinoma was correlated with the grade and stage, level of myometrial invasion, age and survival of the patients. Anti-estrogen and anti-progesteone receptor monoclonal antibodies were applied to paraffin-embedded tissue from hysterectomy specimens of 100 patients with adenocarcinoma of the endometrium. In 34 of the cases the receptors were studied in the endometrium adjacent to the tumor and compared to the nuclear receptor content in the carcinoma. There was a high inverse correlation between the estrogen receptor status and the grade of tumor (R= − 0.45,P= 0.006). The estrogen receptor measured in the endometrium near the tumor showed a negative correlation with the grade of the tumor (R= −0.42,P= 0.013). The estrogen, but not the progesterone, receptor content, was positively related to the age of the patient (P< 0.05). No significant correlation of the receptor status with the depth of myometrial invasion was found, despite the obvious interdependence between the grade and myometrial invasion. The progesterone receptor staining index appeared to be a distinct independent prognostic factor in endometrial cancer. The immunohistochemical analysis of the steroid hormone status in endometrial cancer therefore offers an alternative to the quantitative ligand-binding assay.

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Expression of sex steroid receptor genes and comodulation with retinoid signaling in normal human uroepithelial cells and bladder cancer cell lines

Urologic Oncology Seminars and Original Investigations ◽

10.1016/s1078-1439(98)00011-8 ◽

1997 ◽

Vol 3 (5-6) ◽

pp. 141-147 ◽

Cited By ~ 7

Author(s):

Przemyslaw Waliszewski ◽

Miroslawa K. Waliszewska ◽

George P. Hemstreet ◽

Robert E. Hurst

Keyword(s):

Bladder Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Steroid Receptor ◽

Cancer Cell Lines ◽

Bladder Cancer Cell ◽

Sex Steroid ◽

Retinoid Signaling ◽

Normal Human ◽

Uroepithelial Cells

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Novel Selective Estrogen Receptor Modulator Ameliorates Murine Colitis

International Journal of Molecular Sciences ◽

10.3390/ijms20123007 ◽

2019 ◽

Vol 20 (12) ◽

pp. 3007 ◽

Cited By ~ 1

Author(s):

Lauri Polari ◽

Santeri Anttila ◽

Terhi Helenius ◽

Anu Wiklund ◽

Tero Linnanen ◽

...

Keyword(s):

Estrogen Receptor ◽

Steroid Receptor ◽

Receptor Activation ◽

Selective Estrogen Receptor Modulator ◽

Sex Steroid ◽

Drug Candidate ◽

Inflammatory Monocytes ◽

Receptor Modulator ◽

Anti Inflammatory ◽

Novel Drug

Estrogen-receptor-mediated signaling has been suggested to decrease the inflammatory response in monocyte macrophages. Previously, we showed that a novel selective estrogen receptor modulator (SERM2) promotes anti-inflammatory phenotype of monocytes in vitro. In this study, we demonstrate the potential of SERM2 in amelioration of colitis. We utilized a dextran sodium sulfate (DSS)-induced colitis model in FVB/n mice to demonstrate the effects of orally administered SERM2 on the clinical status of the mice and the histopathological changes in the colon, as well as proportion of Mrc-1 positive macrophages. SERM2 nuclear receptor affinities were measured by radioligand binding assays. Orally administered, this compound significantly alleviated DSS-induced colitis in male mice and induced local estrogen receptor activation in the inflamed colon, as well as promoting anti-inflammatory cytokine expression and infiltration of anti-inflammatory monocytes. We show that this novel drug candidate has an affinity to estrogen receptors α and β and progesterone receptors, but not to glucocorticoid receptor, thus expressing unique binding properties compared to other sex steroid receptor ligands. These results indicate that novel drug candidates to alleviate inflammatory conditions of the colon could be found among sex steroid receptor activating compounds.

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