Immunohistochemical analysis of p53 protein over-expression in endometrial carcinomas: Inverse correlation with sex steroid receptor status

1993 ◽  
Vol 423 (4) ◽  
pp. 265-271 ◽  
Author(s):  
Masafumi Koshiyama ◽  
Ikuo Konishi ◽  
Da-peng Wang ◽  
Masaki Mandai ◽  
Takayuki Komatsu ◽  
...  
Keyword(s):  
P53 Protein ◽  
Inverse Correlation ◽  
Immunohistochemical Analysis ◽  
Steroid Receptor ◽  
Sex Steroid ◽  
Receptor Status ◽  
Over Expression ◽  
Endometrial Carcinomas

An immunohistochemical study of estrogen and progesterone receptors in adenocarcinoma of the endometrium and in the adjacent mucosa

1995 ◽  
Vol 5 (4) ◽  
pp. 275-281 ◽  
Author(s):  
H. Kerner ◽  
E. Sabo ◽  
M. Friedman ◽  
D. Beck ◽  
O. Samare ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Endometrial Cancer ◽  
Progesterone Receptor ◽  
Estrogen Receptor Status ◽  
Endometrial Adenocarcinoma ◽  
Progesterone Receptors ◽  
Myometrial Invasion ◽  
Inverse Correlation ◽  
Immunohistochemical Analysis ◽  
Receptor Status

The immunoperoxidase stain for estrogen and progesterone receptor content in endometrial adenocarcinoma was correlated with the grade and stage, level of myometrial invasion, age and survival of the patients. Anti-estrogen and anti-progesteone receptor monoclonal antibodies were applied to paraffin-embedded tissue from hysterectomy specimens of 100 patients with adenocarcinoma of the endometrium. In 34 of the cases the receptors were studied in the endometrium adjacent to the tumor and compared to the nuclear receptor content in the carcinoma. There was a high inverse correlation between the estrogen receptor status and the grade of tumor (R= − 0.45,P= 0.006). The estrogen receptor measured in the endometrium near the tumor showed a negative correlation with the grade of the tumor (R= −0.42,P= 0.013). The estrogen, but not the progesterone, receptor content, was positively related to the age of the patient (P< 0.05). No significant correlation of the receptor status with the depth of myometrial invasion was found, despite the obvious interdependence between the grade and myometrial invasion. The progesterone receptor staining index appeared to be a distinct independent prognostic factor in endometrial cancer. The immunohistochemical analysis of the steroid hormone status in endometrial cancer therefore offers an alternative to the quantitative ligand-binding assay.

scholarly journals Relationship between AgNOR Proteins, Ki-67 Antigen, p53 Immunophenotype and Differentiation Markers in Archival Breast Carcinomas

1998 ◽  
Vol 17 (4) ◽  
pp. 231-242 ◽  
Author(s):  
Àgnes Bànkfalvi ◽  
Klaus Schmitz ◽  
Tobias Mock ◽  
Mechthild Kemper ◽  
Christiane Cubick ◽  
...  
Keyword(s):  
P53 Protein ◽  
Inverse Correlation ◽  
Tumour Cells ◽  
Growth Fraction ◽  
Breast Carcinomas ◽  
Paraffin Sections ◽  
Differentiation Markers ◽  
Ki 67 ◽  
Proliferation Markers ◽  
Receptor Status

The present study investigated (i) the relationship between standardised morphometric AgNOR parameters (argyrophilic nucleolar organiser region-associated proteins) and MIB1 growth fraction, and (ii) their correlation with immunohistochemical p53, sex steroid receptor status and histopathological differentiation grade in serial paraffin sections from 39 breast carcinomas. Ten sections were double-stained for AgNOR/MIB1. AgNOR parameters correlated significantly with MIB1 growth fraction and p53 protein expression. Significant inverse correlation was found between proliferation markers and oestrogen/progesterone receptor status and histopathological grade. AgNOR expression was significantly higher in cycling (MIB1 positive) tumour cells, than in resting (MIB1 negative) ones, however with exceptions. We conclude, that standardised AgNOR parameters correlate with markers of increased malignant potential in breast carcinomas. However, AgNORs seem to reflect proliferation independent cellular and nucleolar activity of tumour cells, as well. We recommend the use of standardised AgNOR analysis for obtaining sound results in routine paraffin sections.

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