IMMUNE ELECTRON MICROSCOPY OF MONOCLONAL ANTIBODIES TO ALZHEIMER NEUROFIBRILLARY TANGLES

1987 ◽  
Vol 46 (3) ◽  
pp. 333
Author(s):  
M. WRZOLEK ◽  
P. A. MERZ ◽  
R. J. KASCSAK ◽  
I. GRUNDKE-IQBAL ◽  
K. IQBAL ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Neurofibrillary Tangles ◽  
Immune Electron Microscopy ◽  
Alzheimer Neurofibrillary Tangles

A comparison of two indirect immune electron microscopy procedures using monoclonal antibody to localize specific African swine fever virus (ASFV) proteins in infected cells and virus

1983 ◽  
Vol 41 ◽  
pp. 800-801
Author(s):  
G. J. Letchworth III ◽  
T. C. Whyard ◽  
S. H. Wool
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
African Swine Fever Virus ◽  
African Swine Fever ◽  
Grid Method ◽  
Viral Proteins ◽  
Fever Virus ◽  
Immune Electron Microscopy ◽  
Infected Cells

The primary purpose of this study was to devise a procedure in which a large number of monoclonal antibodies could be tested against several African swine fever virus (ASFV) preparations (virus, infected cells). The procedure had to be rapid, use a small sample and provide for some TEM.Monoclonal antibodies were produced by standard methods using myeloma cells and lymphocytes from mice immunized against ASF viral proteins. Antibodies were characterized by immunoprecipitation of radio-labelled viral proteins (Fig 1).The viral preparations used in this study were either infected macrophages, culture fluid supernatant from ASFV-infected macrophages or red blood cells from infected pigs. Each of the three viral preparations was tested by two indirect immune electron microscopy (IEM) methods; a grid method and a test tube method. In the grid method, formvar coated grids were floated on a viral preparation then for one hour each on the mouse monoclonal antibody and finally on ferritin tagged sheep anti-mouse IgG with washes between each step.

Localization of theTrypanosoma cruzi-specific Mr 25,000 antigen by immune electron microscopy using monoclonal antibodies

1986 ◽  
Vol 72 (6) ◽  
pp. 701-707 ◽  
Author(s):  
H. Tachibana ◽  
L. T. Montenegro ◽  
K. Kurihara ◽  
K. Nagakura ◽  
Y. Kaneda ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibodies ◽  
Immune Electron Microscopy
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