Serum immunoglobulin G subclasses in children infected with human immunodeficiency virus type 1

1991 ◽  
Vol 10 (2) ◽  
pp. 134-139 ◽  
Author(s):  
EMMANUEL ROILIDES ◽  
CHARLOTTE BLACK ◽  
CHARLES REIMER ◽  
MARC RUBIN ◽  
DAVID VENZON ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
Serum Immunoglobulin ◽  
Immunoglobulin G Subclasses ◽  
Immunodeficiency Virus

scholarly journals Earlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme Immunoassays

2000 ◽  
Vol 7 (6) ◽  
pp. 872-881 ◽  
Author(s):  
Seiichi Hashida ◽  
Setsuko Ishikawa ◽  
Kazuya Hashinaka ◽  
Ichiro Nishikata ◽  
Shinichi Oka ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Immune Complex ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
Rt Pcr ◽  
Immunodeficiency Virus ◽  
P24 Antigen ◽  
Hiv 1

ABSTRACT For earlier diagnosis of human immunodeficiency virus type 1 (HIV-1) infection, the sensitivities of immune complex transfer enzyme immunoassays for HIV-1 p24 antigen and antibody immunoglobulin G (IgG) to HIV-1 p17 antigen were improved approximately 25- and 90-fold, respectively, over those of the previous immunoassays by performing solid-phase immunoreactions with shaking and increasing the serum sample volumes, and immune complex transfer enzyme immunoassay of antibody IgM to p17 antigen was also performed in the same way as the improved immunoassay of antibody IgG to p17 antigen. By the improved immunoassays, p24 antigen and antibody IgG to p17 antigen were detected earlier in 32 and 53%, respectively, of the HIV-1 seroconversion serum panels tested than before the improvements, and p24 antigen was detected as early as or earlier than HIV-1 RNA by reverse transcriptase-PCR (RT-PCR) in all of the panels tested. In 4 panels out of 19 tested, antibody IgG to p17 antigen or both antibodies IgG and IgM to p17 antigen were detected earlier than p24 antigen and RNA, although the antibody levels declined slightly before their steep increases usually observed after p24 antigen and RNA. Thus, the window period in diagnosis of HIV-1 infection can be shortened by detection of p24 antigen with the improved immunoassay as much as by detection of RNA with RT-PCR and, in some cases, more by detection of antibodies IgG and IgM to p17 antigen with the improved immunoassays than by detections of p24 antigen with the improved immunoassay and RNA with RT-PCR.

scholarly journals Polyclonal Immunoglobulin G from Patients Neutralizes Human Immunodeficiency Virus Type 1 Primary Isolates by Binding Free Virions, but without Interfering with an Initial CD4-Independent Attachment of the Virus to Primary Blood Mononuclear Cells

2003 ◽  
Vol 77 (21) ◽  
pp. 11385-11397 ◽  
Author(s):  
Renaud Burrer ◽  
Sandrine Haessig-Einius ◽  
Anne-Marie Aubertin ◽  
Christiane Moog
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
Mononuclear Cells ◽  
Viral Envelope ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells ◽  
Hiv 1

ABSTRACT We investigated the relationship between human immunodeficiency virus type 1 (HIV-1) primary isolate (PI) antibody-mediated neutralization and attachment to primary blood mononuclear cells (PBMC). Incubation of PIs with immunoglobulin G (IgG) purified from infected patients did not inhibit attachment of the viruses with PBMC, but partial to complete neutralization was achieved. Neutralization of PIs already fixed on the cells was achieved by some IgG samples only and was of limited intensity compared to the former neutralization protocol. On the contrary, the binding of IgG to free virions was shown to be sufficient to reach potent neutralization, as the infectivity of IgG-PI complexes purified from the bulk of antibodies before addition to PBMC was strongly diminished compared to mock-treated controls. Monoclonal antibodies to the CDR2 domain of CD4 completely inhibited the infection of PBMC without interfering with the attachment of PIs to the cells, suggesting that, under these experimental conditions, the initial attachment of viruses to PBMC involves alternative cellular receptors. This initial interaction may also involve other components of the viral envelope than gp120, as partial depletion of the surface glycoproteins of primary viral particles that resulted in an almost complete loss of infectivity did not impair attachment to PBMC. A limited inhibition of attachment was observed when interfering with putative interactions with cellular heparan sulfate, whereas no effect was observed for cellular CD147 or nucleolin or for virion-incorporated cyclophilin A. Altogether, our results favor a mechanism of neutralization of HIV-1 PIs by polyclonal IgG where antibodies predominantly bind free virions and neutralize without interfering with the attachment to PBMC, which, in this model, is mainly CD4 independent.

scholarly journals Utilization of Immunoglobulin G Fc Receptors by Human Immunodeficiency Virus Type 1: a Specific Role for Antibodies against the Membrane-Proximal External Region of gp41

2009 ◽  
Vol 83 (15) ◽  
pp. 7397-7410 ◽  
Author(s):  
Lautaro G. Perez ◽  
Matthew R. Costa ◽  
Christopher A. Todd ◽  
Barton F. Haynes ◽  
David C. Montefiori
Keyword(s):  
Human Immunodeficiency Virus ◽  
Monoclonal Antibodies ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
External Region ◽  
Specific Antibodies ◽  
Immunodeficiency Virus ◽  
Hiv 1

ABSTRACT Receptors (FcγRs) for the constant region of immunoglobulin G (IgG) are an important link between humoral immunity and cellular immunity. To help define the role of FcγRs in determining the fate of human immunodeficiency virus type 1 (HIV-1) immune complexes, cDNAs for the four major human Fcγ receptors (FcγRI, FcγRIIa, FcγRIIb, and FcγRIIIa) were stably expressed by lentiviral transduction in a cell line (TZM-bl) commonly used for standardized assessments of HIV-1 neutralization. Individual cell lines, each expressing a different FcγR, bound human IgG, as evidence that the physical properties of the receptors were preserved. In assays with a HIV-1 multisubtype panel, the neutralizing activities of two monoclonal antibodies (2F5 and 4E10) that target the membrane-proximal external region (MPER) of gp41 were potentiated by FcγRI and, to a lesser extent, by FcγRIIb. Moreover, the neutralizing activity of an HIV-1-positive plasma sample known to contain gp41 MPER-specific antibodies was potentiated by FcγRI. The neutralizing activities of monoclonal antibodies b12 and 2G12 and other HIV-1-positive plasma samples were rarely affected by any of the four FcγRs. Effects with gp41 MPER-specific antibodies were moderately stronger for IgG1 than for IgG3 and were ineffective for Fab. We conclude that FcγRI and FcγRIIb facilitate antibody-mediated neutralization of HIV-1 by a mechanism that is dependent on the Fc region, IgG subclass, and epitope specificity of antibody. The FcγR effects seen here suggests that the MPER of gp41 could have greater value for vaccines than previously recognized.

scholarly journals Seroincidence of Recent Human Immunodeficiency Virus Type 1 Infections in China

2007 ◽  
Vol 14 (10) ◽  
pp. 1384-1386 ◽  
Author(s):  
Yao Xiao ◽  
Yan Jiang ◽  
Jigang Feng ◽  
Wenyan Xu ◽  
Minjie Wang ◽  
...  
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Immunoglobulin G ◽  
Cross Sectional ◽  
Subtype B ◽  
Immunodeficiency Virus

ABSTRACT A subtype B, E, and D immunoglobulin G capture immunoassay shows promise as a tool for estimating human immunodeficiency virus type 1 seroincidence from cross-sectional surveys, but the test-specific limitations suggest that an adjustment is necessary, and further validation of the assay with populations with divergent subtypes is needed.

Sign in / Sign up

Export Citation Format

Share Document