PLASMA CONCENTRATION OF BIOLOGICALLY ACTIVE FIBRONECTIN AND FIBRONECTIN BOUND TO GELATIN-LIKE MATERIAL IN A PORCINE MODEL OF HYPERDYNAMIC ENDOTOXIC SHOCK

The objective of this randomized, open-label, single-dose, two-phase crossover study was to determine the effect of ascorbic acid on pharmacokinetics of ganoderic acid A, an important biologically active triterpenoid compound with anticancer activities, following oral administration of water extract of fruiting bodies of Ling Zhi in 12 healthy male subjects. Each subject was randomized to receive either one of the two regimens: (1) a single dose of 3,000 mg of the Ling Zhi preparation or (2) a single dose of 3,000 mg of the Ling Zhi preparation in combination with 2,500 mg of ascorbic acid. After a washout period of at least two weeks, subjects were switched to receive the alternate regimen. Blood samples were collected in each phase immediately before dosing and at specific time points for 8 hours after dosing. Plasma ganoderic acid A concentrations were quantified using liquid chromatography-mass spectrometry (LC-MS). The pharmacokinetic parameters analyzed were maximal plasma concentration (Cmax), time to reach peak concentration (Tmax), area under the plasma concentration-time curve (AUC), and half-life (t1/2). An oral coadministration of ascorbic acid with Ling Zhi preparation did not significantly alter the pharmacokinetic parameters of ganoderic acid A in healthy male subjects.

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Subunit S and A Ratio in Congenital and Acquired Deficiency of Factor XIII

10.1055/s-0039-1687428 ◽

1979 ◽

Author(s):

T. Barbui ◽

F. Rodeghiero ◽

E. Dini

Keyword(s):

Plasma Concentration ◽

Disseminated Intravascular Coagulation ◽

Factor Xiii ◽

Active Component ◽

Average Concentration ◽

Biologically Active ◽

Carrier Protein ◽

Factor Xiii Deficiency ◽

Subunit A ◽

Intravascular Coagulation

Factor XIII contains two subunits :subunit A, the biologically active component, and subunit S, the “carrier” protein. In 7 homozygotes of factor XIII deficiency, the subunit A was immunologically undetectable while subunit S was on average 32%. The 29 heterozygotes had an average concentration of subunit A 35%, while subunit S was on average 66%. In the heterozygote group, the ratio S/A was 1.93.In 32 patients with acute myeloblastic leukemia the ratio S/A was found impaired. The group of patients with severe disseminated intravascular coagulation (DIC) had a ratio 2.08 and the cases without a manifest 0IC had a ratio 2.12.These findings seem to support the view that subunit S plasma concentration drops in accordance to subunit A, even though the reduction of this active componentis due to different mechanisms.

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Haemodynamic Effects of Nitric Oxide Synthase Inhibitors in an Improved Porcine Model of Endotoxic Shock

Clinical Science ◽

10.1042/cs089045pb ◽

1995 ◽

Vol 89 (s33) ◽

pp. 45P-46P

Author(s):

NA Herity ◽

JD Allen ◽

B Silke ◽

AAJ Adgey

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Porcine Model ◽

Endotoxic Shock ◽

Haemodynamic Effects ◽

Nitric Oxide Synthase Inhibitors ◽

Oxide Synthase

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GROWTH HORMONE RELEASING FACTOR AND THE BIOASSAY-RADIOIMMUNOASSAY PARADOX REVISITED

Acta Endocrinologica ◽

10.1530/acta.0.0860071 ◽

1977 ◽

Vol 86 (1) ◽

pp. 71-80 ◽

Cited By ~ 8

Author(s):

Morton A. Vodian ◽

Charles S. Nicoll

Keyword(s):

Growth Hormone ◽

Plasma Concentration ◽

Quantitative Agreement ◽

Biologically Active ◽

Male Rats ◽

Growth Hormone Releasing Factor ◽

Granule Fraction ◽

Rat Tibia ◽

The Relationship

ABSTRACT Relationships between depletion of growth hormone (GH) from the rat adenohypophysis and its release into the circulation were re-evaluated using bioassay (BA: rat tibia test) and radioimmunoassay (RIA). Anaesthetized male rats were injected with a putative growth hormone releasing factor ("GRF") and killed 15 and 30 min later. For the assays, the plasma and adenohypophyses were pooled; the latter were separated into granule and cytosol fractions prior to assay. Fifteen minutes after "GRF" injection the BA recorded a 75 % depletion of GH from the granular fraction but the RIA measured a loss of only 13 %. By 30 min both assays recorded restoration of pituitary GH. Only slight changes occurred in the levels of BA- or RIA-detectable GH in the cytosol fractions. The massive depletion of bioassayable GH from the granule fraction was accompanied by a large increase (3.2 mU/ml) in the plasma concentration of BA-detectable hormone by 15 min. The slight depletion of RIA-detectable GH that occurred during this time was associated with a meagre increase (0.05 mU/ml) in the plasma level of immunoreactive GH. The plasma concentration of BA- and RIA-detectable GH did not change significantly between 15 and 30 min. Analysis of the relationship between depletion of GH from the pituitary and its release into the extrapituitary compartment disclosed that the BA recordings of these events were in excellent quantitative agreement but RIA measurements were not. The results confirm that biologically active GH can be depleted from and released by the adenohypophysis in vivo independently of RIA-detectable GH.

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