MassARRAY Offers Highly Sensitive Type-specific Detection and Quantification of Human Papillomavirus

2006 ◽  
Vol 10 (3) ◽  
pp. 186
Author(s):  
D.A. Patel ◽  
M.T. Ruffin ◽  
K. Yang ◽  
Y. Tang ◽  
Y.J. Shih ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Specific Detection ◽  
Highly Sensitive ◽  
Detection And Quantification

Highly sensitive and specific detection of Giardia duodenalis, Entamoeba histolytica, and Cryptosporidium spp. in human stool samples by the BD MAX™ Enteric Parasite Panel

2017 ◽  
Vol 117 (2) ◽  
pp. 447-451 ◽  
Author(s):  
Marijo Parčina ◽  
Ingrid Reiter-Owona ◽  
Frank P. Mockenhaupt ◽  
Valerija Vojvoda ◽  
Jean Bosco Gahutu ◽  
...  
Keyword(s):  
Entamoeba Histolytica ◽  
Giardia Duodenalis ◽  
Specific Detection ◽  
Cryptosporidium Spp ◽  
Highly Sensitive ◽  
Stool Samples ◽  
Human Stool

PAS1-modified optical SIS sensor for highly sensitive and specific detection of toluene

2019 ◽  
Vol 141 ◽  
pp. 111469
Author(s):  
Tran Thi Dung ◽  
Ui Jin Lee ◽  
Myung Hee Kim ◽  
Kyoon Eon Kim ◽  
Hyun Mo Cho ◽  
...  
Keyword(s):  
Specific Detection ◽  
Highly Sensitive

scholarly journals Development and validation of an LC-MS/MS method for detection and quantification of in vivo derived metabolites of [Pyr1]apelin-13 in humans

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Duuamene Nyimanu ◽  
Richard G. Kay ◽  
Petra Sulentic ◽  
Rhoda E. Kuc ◽  
Philip Ambery ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Healthy Human ◽  
Highly Sensitive ◽  
Human Volunteers ◽  
Reproducible Method ◽  
Human Cardiovascular System ◽  
Cardiovascular Therapeutics ◽  
Development And Validation ◽  
Detection And Quantification

Abstract[Pyr1]apelin-13 is the predominant apelin peptide isoform in the human cardiovascular system and plasma. To date, few studies have investigated [Pyr1]apelin-13 metabolism in vivo in rats with no studies examining its stability in humans. We therefore aimed to develop an LC-MS/MS method for detection and quantification of intact [Pyr1]apelin-13 and have used this method to identify the metabolites generated in vivo in humans. [Pyr1]apelin-13 (135 nmol/min) was infused into six healthy human volunteers for 120 minutes and blood collected at time 0 and 120 minutes after infusion. Plasma was extracted in the presence of guanidine hydrochloride and analysed by LC-MS/MS. Here we report a highly sensitive, robust and reproducible method for quantification of intact [Pyr1]apelin-13 and its metabolites in human plasma. Using this method, we showed that the circulating concentration of intact peptide was 58.3 ± 10.5 ng/ml after 120 minutes infusion. We demonstrated for the first time that in humans, [Pyr1]apelin-13 was cleaved from both termini but the C-terminal was more susceptible to cleavage. Consequently, of the metabolites identified, [Pyr1]apelin-13(1–12), [Pyr1]apelin-13(1–10) and [Pyr1]apelin-13(1–6) were the most abundant. These data suggest that apelin peptides designed for use as cardiovascular therapeutics, should include modifications that minimise C-terminal cleavage.

Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

2015 ◽  
Vol 49 (15) ◽  
pp. 9256-9263 ◽  
Author(s):  
Feng Tan ◽  
Nuvia Maria Saucedo ◽  
Pankaj Ramnani ◽  
Ashok Mulchandani
Keyword(s):  
Water Samples ◽  
Label Free ◽  
Specific Detection ◽  
Highly Sensitive

Quantitative and specific detection of cancer-related microRNAs in living cells using surface-enhanced Raman scattering imaging based on hairpin DNA-functionalized gold nanocages

The Analyst ◽  
2019 ◽  
Vol 144 (24) ◽  
pp. 7250-7262 ◽  
Author(s):  
Zhenyu Wang ◽  
Jin Xue ◽  
Caili Bi ◽  
Heng Xin ◽  
Youwei Wang ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Surface Enhanced Raman Scattering ◽  
Living Cells ◽  
Specific Detection ◽  
Hairpin Dna ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Gold Nanocages

A highly sensitive surface-enhanced Raman scattering (SERS) strategy based on hairpin DNA-functionalized gold nanocages for the detection of intracellular miR-125a-5p.

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