Real-Time Imaging and Quantification of Bioluminescent Bacteria in Root Canals In Vitro

2004 ◽  
Vol 30 (12) ◽  
pp. 893-898 ◽  
Author(s):  
C SEDGLEY ◽  
B APPLEGATE ◽  
A NAGEL ◽  
D HALL
Keyword(s):  
Real Time ◽  
Bioluminescent Bacteria ◽  
Real Time Imaging ◽  
Root Canals

Real time imaging reveals a peroxisomal reticulum in living cells

2000 ◽  
Vol 113 (20) ◽  
pp. 3663-3671 ◽  
Author(s):  
M. Schrader ◽  
S.J. King ◽  
T.A. Stroh ◽  
T.A. Schroer
Keyword(s):  
Real Time ◽  
Cytoplasmic Dynein ◽  
Living Cells ◽  
Real Time Imaging ◽  
Peroxisomal Targeting ◽  
Microtubule Motor ◽  
Targeting Signal ◽  
Peroxisomal Targeting Signal

We have directly imaged the dynamic behavior of a variety of morphologically different peroxisomal structures in HepG2 and COS-7 cells transfected with a construct encoding GFP bearing the C-terminal peroxisomal targeting signal 1. Real time imaging revealed that moving peroxisomes interacted with each other and were engaged in transient contacts, and at higher magnification, tubular peroxisomes appeared to form a peroxisomal reticulum. Local remodeling of these structures could be observed involving the formation and detachment of tubular processes that interconnected adjacent organelles. Inhibition of cytoplasmic dynein based motility by overexpression of the dynactin subunit, dynamitin (p50), inhibited the movement of peroxisomes in vivo and interfered with the reestablishment of a uniform distribution of peroxisomes after recovery from nocodazole treatment. Isolated peroxisomes moved in vitro along microtubules in the presence of a microtubule motor fraction. Our data reveal that peroxisomal behavior in vivo is significantly more dynamic and interactive than previously thought and suggest a role for the dynein/dynactin motor in peroxisome motility.

scholarly journals Real-Time Imaging of Bacteria/Osteoblast Dynamic Coculture on Bone Implant Material in an in Vitro Postoperative Contamination Model

2019 ◽  
Vol 5 (7) ◽  
pp. 3260-3269 ◽  
Author(s):  
Victor Prévost ◽  
Karine Anselme ◽  
Olivier Gallet ◽  
Mathilde Hindié ◽  
Tatiana Petithory ◽  
...  
Keyword(s):  
Real Time ◽  
Bone Implant ◽  
Real Time Imaging ◽  
Implant Material

Characterization and real-time imaging of the FTLD-related protein aggregation induced by amyloidogenic peptides

2015 ◽  
Vol 51 (41) ◽  
pp. 8652-8655 ◽  
Author(s):  
Ruei-Yu He ◽  
Yi-Chen Huang ◽  
Chao-Wei Chiang ◽  
Yu-Ju Tsai ◽  
Ting-Juan Ye ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Real Time ◽  
Live Cells ◽  
Related Protein ◽  
Amyloid Fibers ◽  
Real Time Imaging ◽  
C Terminus ◽  
Amyloidogenic Peptides

Q/N- and G-rich polypeptides from the TDP-43 C-terminus formed amyloid fibers in vitro and induced the aggregation of the transfected TDP-43-EGFP in live cells.

scholarly journals Abstract 35: 3D in vitro system for immuno-oncology: Real-time imaging of drug delivery, tumoroids, and immune cell activity

2019 ◽  
Author(s):  
Eric O. McGhee ◽  
Alex J. McGhee ◽  
Derek L. Hood ◽  
Kylie E. Van Meter ◽  
Juan M. Urueña ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Real Time ◽  
Immune Cell ◽  
Cell Activity ◽  
In Vitro System ◽  
Real Time Imaging

REAL TIME IMAGING OF HEMOLYSIS IN IN-VITRO WITH CHEMICAL LUMINESCENCE

ASAIO Journal ◽  
2005 ◽  
Vol 51 (2) ◽  
pp. 8A
Author(s):  
Shuichi Mochizuki ◽  
Koki Takiura ◽  
Yusuke Abe ◽  
Kou Imachi
Keyword(s):  
Real Time ◽  
Real Time Imaging
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