The Influence of Canal Curvature on the Mechanical Efficacy of Root Canal Irrigation In Vitro Using Real-Time Imaging of Bioluminescent Bacteria

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

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Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants

The Journal of Contemporary Dental Practice ◽

10.5005/jcdp-9-5-68 ◽

2008 ◽

Vol 9 (5) ◽

pp. 68-75 ◽

Cited By ~ 4

Author(s):

Rafael Brandao Ferreira ◽

Melissa Andreia Marchesan ◽

Yara Teresinha Silva-Sousa ◽

Manoel Sousa-Neto

Keyword(s):

Sodium Hypochlorite ◽

Root Canal ◽

Distilled Water ◽

Chlorhexidine Digluconate ◽

Canal Irrigation ◽

Root Canals ◽

Root Canal Irrigation ◽

Debris Removal ◽

Group 2

Abstract Aim The aim of this in vitro study was to evaluate the cleaning capacity of different auxiliary chemical substances energized with ultrasound in radicular dentin using morphologic analysis. Methods and Materials Twenty-eight single-canal, mandibular incisors were prepared with the Hero 642 canal preparation system following the sequence: 25/12, 30/06, 35/02, and 40/02, 1 mm from the apex. The specimens were then divided into four groups of seven teeth. During biomechanical preparation the teeth were irrigated with 2 ml of distilled water between files. Each group of specimens (n=7) received a final irrigation with 100 ml of the following irrigants that were activated with ultrasound for 3 minutes: Group 1- distilled water; Group 2- 0.2% chlorhexidine digluconate (CHX); Group 3- 2.5% sodium hypochlorite (NaOCl); and Group 4-the filtrate obtained after combining 0.2% CHX and 2.5% NaOCl. The apical portions of the root canals from each group were then submitted to histological processing and analyzed using optical microscopy. Results Results showed statistical differences between the groups (p<0.01). Groups 1 and 2 (distilled water and 0.2% CHX respectively) were statistically similar in terms of a greater amount of debris, whereas 2.5% NaOCl and the filtrate were more efficient in removal of debris. Conclusion There is no additional benefit in terms of debris removal from root canal walls by irrigating with the filtrate obtained from the combination of NaOCl and CHX when compared to using NaOCl alone. Clinical Significance The findings of this study suggest the time and expense of preparing and using a combination of NaOCl and CHX is not warranted compared to using NaOCl alone for root canal irrigation. Citation Ferreira RB, Marchesan MA, Silva-Sousa YT, Sousa-Neto M. Effectiveness of Root Canal Debris Removal Using Passive Ultrasound Irrigation with Chlorhexidine Digluconate or Sodium Hypochlorite Individually or in Combination as Irrigants. J Contemp Dent Pract 2008 July; (9)5:068-075.

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An in vitro evaluation of the degree of pulp tissue dissolution through different root canal irrigation protocols

Journal of Conservative Dentistry ◽

10.4103/jcd.jcd_49_18 ◽

2018 ◽

Vol 21 (2) ◽

pp. 175 ◽

Cited By ~ 3

Author(s):

Alfredo Iandolo ◽

Massimo Amato ◽

Giuseppe Pantaleo ◽

Dina Abtellatif ◽

Andrea Blasi ◽

...

Keyword(s):

Root Canal ◽

Pulp Tissue ◽

In Vitro Evaluation ◽

Canal Irrigation ◽

Root Canal Irrigation

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ANTIBACTERIAL EFFECTIVENESS OF NONI EXTRACT (MORINDA CITRIFOLIA L.) ON THE GROWTH OF FUSOBACTERIUM NUCLEATUM (ATCC® 25586™) AS AN ALTERNATIVE MATERIAL FOR ROOT CANAL IRRIGATION- AN IN-VITRO STUDY

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2019/231 ◽

2019 ◽

Vol 8 (13) ◽

pp. 1046-1048 ◽

Cited By ~ 1

Author(s):

Trelia Boel ◽

Dennis Dennis

Keyword(s):

Root Canal ◽

In Vitro Study ◽

Fusobacterium Nucleatum ◽

Vitro Study ◽

Morinda Citrifolia ◽

Canal Irrigation ◽

Root Canal Irrigation ◽

Alternative Material

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In Vitro Evaluation of Different Irrigation Protocols on Intracanal Smear Layer Removal in Teeth with or without Pre-Endodontic Proximal Wall Restoration

Journal of Clinical Medicine ◽

10.3390/jcm9103325 ◽

2020 ◽

Vol 9 (10) ◽

pp. 3325

Author(s):

Naji Kharouf ◽

Eugenio Pedullà ◽

Giusy Rita Maria La Rosa ◽

Frédéric Bukiet ◽

Salvatore Sauro ◽

...

Keyword(s):

Root Canal ◽

Resin Composite ◽

Smear Layer ◽

Canal Irrigation ◽

Layer Removal ◽

Apical Level ◽

Root Canal Irrigation ◽

Significant Difference ◽

Sonic Activation

To investigate the influence of pre-endodontic coronal wall restoration on smear layer removal during different root canal irrigation strategies, single-root premolars were prepared with a mesio-occlusal cavity. Half were left untreated (G1), while the mesial walls of the remaining half were reconstructed using a resin composite (G2). The specimens were divided into control (ctrl) groups, which used the conventional needle irrigation method, and four experimental subgroups according to irrigation strategy: Sonic activation using the Endoactivator, sonic activation using the EQ-S, mechanical activation using the XP-Endo finisher, and ultrasonic activation using the EndoUltra. Smear layer removal was assessed through SEM and the results were statistically analyzed. At the coronal and middle thirds, no significant difference (p > 0.05) was detected for G1 and G2, except for the control subgroups (G1ctrl vs G2 ctrl) at the middle third. At the apical third, the smear layer removal was significantly greater for G2 than G1. In G1, both at the middle and apical level, EQ-S and EndoUltra showed greater smear layer removal (p < 0.05) compared to the others G1 subgroups. In G2, at the apical level, the EQ-S and EndoUltra were the most effective in smear layer removal. Pre-endodontic coronal wall restoration may improve the smear layer removal during root canal irrigation.

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Improving the disinfection of lateral microcanals in endodontic dental treatment

Российский стоматологический журнал ◽

10.17816/1728-2802-2020-24-6-364-368 ◽

2020 ◽

Vol 24 (6) ◽

pp. 364-368

Author(s):

Olga Ye. Bekjanova ◽

N. Sh. Abdulkhakova ◽

M. M. Astanokulova

Keyword(s):

Root Canal ◽

Dental Treatment ◽

Irrigation System ◽

Effective Parameters ◽

Dentinal Tubules ◽

Canal Irrigation ◽

In Vitro Experiment ◽

Antiseptic Solution ◽

Root Canal Irrigation

We studied the penetration depth of an antiseptic introduced in the dentin of the tooth by using various methods. An in vitro experiment was performed to determine effective parameters of ultraphonophoresis and electrophoresis, providing the maximum-diffusion antiseptic Decasan in the dentin of the tooth. Compared with the control, three physiotherapy sessions of the root canal significantly increased in-depth penetration in single-rooted teeth. The inclusion of Decasan in the endodontic treatment of patients with chronic apical periodontitis for drug processing the root canal irrigation system is effective because it promotes deep diffusion of antiseptic solution in dentinal tubules.

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