RAT UROTHELIUM: IMPROVED TECHNIQUES FOR SERIAL CULTIVATION, EXPANSION, FREEZING AND RECONSTITUTION ONTO ACELLULAR MATRIX

To investigate the biocompatibility of polylactic acid-glycolic acid copolymer (PLGA) and PLGA/gelatin scaffolds and their suitability for tubular urethral replacement in a canine model. PLGA and PLGA/gelatin scaffolds was constructed by electrospinning. Microstructural differences between the scaffolds was examined by Scanning electron microscopy (SEM) followed by mechanical properties testing. Biocompatibility of the material was evaluated using SEM 4, 8, 12 and 72 h after PLGA and PLGA/gelatin scaffolds co-culture with urothelial cells. And confocal analysis was also used to showed the cell adhesive and growth at 12 h. Approximately 2 cm of the anterior urethra of twelve dogs were removed and replaced with a scaffold. After the surgery for 1 month performed urethrography and for 3 month perform hematoxylin–eosin (H&E) and Masson. The results indicated that PLGA and PLGA/gelatin scaffolds had a void microfilament structure, similar to that of normal acellular matrix tissue. And the tensile strength was decreased whereas the tensile deformation and suture retention strength was increased in PLGA/gelatin scaffolds compared to that in PLGA scaffolds Urothelial cells grew well on both scaffolds. Postoperatively, animals recovered well and urinated spontaneously. However, urethrography showed varying degrees of urethral strictures in the reconstructed urethras. H&E and Masson showed that multilayer urothelial cells were formed in both the proximal and distal segments of the reconstructed urethras but without continuity. There was a small amount of smooth muscle and blood vessels under the epithelium, but regenerative urothelial cells at the midpoint of the reconstructed segment did not continue. Lots of lymphocyte infiltration was observed under the epithelium, some collagen tissue was deposited under the neo-urethral epithelium were observed. In conclusion, PLGA and PLGA/gelatin scaffolds are not suitable for tubularized urethral replacement in the canine model.

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Acellular Matrix–Based Cartilage Regeneration Techniques for Osteochondral Repair

Operative Techniques in Orthopaedics ◽

10.1053/j.oto.2014.02.002 ◽

2014 ◽

Vol 24 (1) ◽

pp. 14-18 ◽

Cited By ~ 3

Author(s):

Elizaveta Kon ◽

Giuseppe Filardo ◽

Francesco Perdisa ◽

Giulia Venieri ◽

Maurilio Marcacci

Keyword(s):

Cartilage Regeneration ◽

Acellular Matrix ◽

Osteochondral Repair

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HETEROLOGOUS ACELLULAR MATRIX GRAFT FOR RECONSTRUCTION OF THE RABBIT URETHRA: HISTOLOGICAL AND FUNCTIONAL EVALUATION

The Journal of Urology ◽

10.1016/s0022-5347(05)66301-x ◽

2001 ◽

Vol 165 (6 Part 1) ◽

pp. 2096-2102 ◽

Cited By ~ 26

Author(s):

KARL-DIETRICH SIEVERT ◽

JÖRG WEFER ◽

MUSTAFA EMRE BAKIRCIOGLU ◽

LORA NUNES ◽

RAJVIR DAHIYA ◽

...

Keyword(s):

Functional Evaluation ◽

Acellular Matrix ◽

Rabbit Urethra

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Comparison of morphological and functional restoration between asymmetric bilayer chitosan and bladder acellular matrix graft for bladder augmentation in a rat model

RSC Advances ◽

10.1039/c7ra07601k ◽

2017 ◽

Vol 7 (67) ◽

pp. 42579-42589 ◽

Cited By ~ 6

Author(s):

Dongdong Xiao ◽

Qiong Wang ◽

Hao Yan ◽

An Qin ◽

Xiangguo Lv ◽

...

Keyword(s):

Smooth Muscle ◽

Rat Model ◽

Muscle Regeneration ◽

Bladder Capacity ◽

Functional Restoration ◽

Bladder Augmentation ◽

Acellular Matrix ◽

Bladder Reconstruction

Asymmetric bilayer chitosan promoted bladder reconstruction with enhanced smooth muscle regeneration and angiogenesis, and functional restoration with augmented bladder capacity.

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Tissue Engineering of Pulmonary Heart Valves on Allogenic Acellular Matrix Conduits : In Vivo Restoration of Valve Tissue

Circulation ◽

10.1161/01.cir.102.suppl_3.iii-50 ◽

2000 ◽

Vol 102 (Supplement 3) ◽

pp. III-50-III-55 ◽

Cited By ~ 156

Author(s):

G. Steinhoff ◽

U. Stock ◽

N. Karim ◽

H. Mertsching ◽

A. Timke ◽

...

Keyword(s):

Tissue Engineering ◽

Heart Valves ◽

Acellular Matrix ◽

Valve Tissue

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MP-10.06: Urethral Reconstruction Using Oral Keratinocyte-Seeded Bladder Acellular Matrix Grafts (BAMGs)

Urology ◽

10.1016/j.urology.2009.07.924 ◽

2009 ◽

Vol 74 (4) ◽

pp. S89

Author(s):

C. Li ◽

Y. Xu ◽

L. Song ◽

Q. Fu ◽

L. Cui ◽

...

Keyword(s):

Urethral Reconstruction ◽

Acellular Matrix

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Therapeutic Effects of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Combined with Cartilage Acellular Matrix Mediated Via Bone Morphogenic Protein 6 in a Rabbit Model of Articular Cruciate Ligament Transection

Stem Cell Reviews and Reports ◽

10.1007/s12015-020-09958-9 ◽

2020 ◽

Vol 16 (3) ◽

pp. 596-611 ◽

Cited By ~ 1

Author(s):

Hyo-Jin Jeon ◽

Kyung-Ae Yoon ◽

Eun Suk An ◽

Tae-Wook Kang ◽

Yun-Beom Sim ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cord Blood ◽

Umbilical Cord Blood ◽

Cruciate Ligament ◽

Rabbit Model ◽

Human Umbilical Cord Blood ◽

Human Umbilical Cord ◽

Therapeutic Effects ◽

Acellular Matrix

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Rhamnolipids stabilize quorum sensing mediated cooperation in Pseudomonas aeruginosa

FEMS Microbiology Letters ◽

10.1093/femsle/fnaa080 ◽

2020 ◽

Vol 367 (10) ◽

Cited By ~ 2

Author(s):

Rodolfo García-Contreras ◽

Daniel Loarca ◽

Caleb Pérez-González ◽

J Guillermo Jiménez-Cortés ◽

Abigail Gonzalez-Valdez ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Quorum Sensing ◽

Carbon Source ◽

Public Goods ◽

Sole Carbon Source ◽

Regulatory Genes ◽

Chronic Infections ◽

Toxic Metabolites ◽

Serial Cultivation ◽

Selection Of

ABSTRACT Pseudomonas aeruginosa is one of the main models to study social behaviors in bacteria since it synthesizes several exoproducts, including exoproteases and siderophores and release them to the environment. Exoproteases and siderophores are public goods that can be utilized by the individuals that produce them but also by non-producers, that are considered social cheaters. Molecularly exoprotease cheaters are mutants in regulatory genes such as lasR, and are commonly isolated from chronic infections and selected in the laboratory upon serial cultivation in media with protein as a sole carbon source. Despite that the production of exoproteases is exploitable, cooperators have also ways to restrict the growth and selection of social cheaters, for instance by producing toxic metabolites like pyocyanin. In this work, using bacterial competitions, serial cultivation and growth assays, we demonstrated that rhamnolipids which production is regulated by quorum sensing, selectively affect the growth of lasR mutants and are able to restrict social cheating, hence contributing to the maintenance of cooperation in Pseudomonas aeruginosa populations.

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