scholarly journals PD13-06 PHAGE DISPLAY-DERIVED PEPTIDE-BASED NEAR-INFRARED IMAGING PROBE FOR INTRAOPERATIVE BLADDER CANCER DETECTION POST-INSTILLATION IN HUMANS

2019 ◽  
Vol 201 (Supplement 4) ◽  
Author(s):  
Li Peng* ◽  
Wenting Shang ◽  
Jie Tian ◽  
Wanhai Xu
2004 ◽  
Vol 9 (4) ◽  
pp. 767 ◽  
Author(s):  
Stavros G. Demos ◽  
Regina Gandour-Edwards ◽  
Rajen Ramsamooj ◽  
Ralph deVere White

2001 ◽  
Vol 40 (19) ◽  
pp. 3288 ◽  
Author(s):  
Quing Zhu ◽  
Nan Guang Chen ◽  
Daqing Piao ◽  
Puyun Guo ◽  
XiaoHui Ding

2021 ◽  
Author(s):  
Wenting Shang ◽  
Li Peng ◽  
Kunshan He ◽  
Pengyu Guo ◽  
Han Deng ◽  
...  

Abstract Bladder cancer is the fifth most common malignancy in humans. Cystoscopy under white light imaging is the gold standard for bladder cancer diagnosis, but these tumors are difficult to visualize and can be overlooked, resulting in high recurrence rates. We previously developed a phage display-derived peptide-based near-infrared imaging probe, PLSWT7-DMI, which binds specifically to bladder cancer cells and is nontoxic to animals. Here, we report the first-in-human application of this probe for near-infrared fluorescence endoscopic detection of bladder cancer. The purity, efficacy, safety, and nontoxicity of the probe were confirmed prior to its clinical application. Twenty-two patients diagnosed with suspected non-muscle invasive bladder cancer were enrolled in the present study. Following intravesical administration of the probe, the entire mucosa was imaged under white and near-infrared imaging using an in-house developed endoscope that could switch between these two modes. The illuminated lesions under near-infrared light were biopsied and sent for histopathological examination. We observed a 5.1-fold increase in the fluorescence intensity in the tumor samples compared to normal tissue, and the probe demonstrated a sensitivity and specificity of 91.2% and 90%, respectively. Common diagnostic challenges, such as small satellite tumors, carcinoma in situ , and benign suspicious mucosa, were visualized and could be distinguished from cancer. Further, no adverse effects were observed in humans. These first-in-human results indicate that PLSWT7-DMI-based near-infrared fluorescence endoscopy is a safe and effective approach for the improved detection of bladder cancer, and may enable thorough resection to prevent recurrence.


2006 ◽  
Author(s):  
Deborah W. Knapp ◽  
Larry G. Adams ◽  
Jacqueline D. Niles ◽  
Michael D. Lucroy ◽  
Jose Ramos-Vara ◽  
...  

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