scholarly journals Lack of Expression of the Epstein-Barr Virus (EBV) Gene Products, EBERs, EBNA1, LMP1, and LMP2A, in Breast Cancer Cells

2002 ◽  
Vol 82 (9) ◽  
pp. 1193-1199 ◽  
Author(s):  
C G Deshpande ◽  
S Badve ◽  
N Kidwai ◽  
R Longnecker
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epstein Barr Virus ◽  
Gene Products ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals Dysregulation of HER2/HER3 Signaling Axis in Epstein-Barr Virus-Infected Breast Carcinoma Cells

2007 ◽  
Vol 81 (11) ◽  
pp. 5705-5713 ◽  
Author(s):  
Jiun-Han Lin ◽  
Ching-Hwa Tsai ◽  
Jan-Show Chu ◽  
Jeou-Yuan Chen ◽  
Kenzo Takada ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Epstein Barr Virus ◽  
Soft Agar ◽  
Signaling Cascades ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr ◽  
Tumorigenic Activity

ABSTRACT The role of Epstein-Barr virus (EBV) in the pathogenesis of breast cancer has been of long-standing interest to the field. Breast epithelial cells can be infected by EBV through direct contact with EBV-bearing lymphoblastoid cells, and EBV infection has recently been shown to confer breast cancer cells an increased resistance to chemotherapeutic drugs. In this study, we established EBV-infected breast cancer MCF7 and BT474 cells and demonstrated that EBV infection promotes tumorigenic activity of breast cancer cells. Firstly, we showed that the EBV-infected MCF7-A and BT474-A cells exhibited increased anchorage-independent growth in soft agar. The increased colony formation capacity in soft agar was associated with increased expression and activation of HER2/HER3 signaling cascades, as evidenced by the findings that the treatment of HER2 antibody trastuzumab (Herceptin), phosphatidylinositol 3-kinase inhibitor, or MEK inhibitor completely abolished the tumorigenic capacity. In the EBV-infected breast cancer cells, the expression of EBV latency genes including EBNA1, EBER1, and BARF0 was detected. We next showed that BARF0 alone was sufficient to efficiently up-regulate HER2/HER3 expression and promoted tumorigenic activity in MCF7 and BT474 cells by the use of both overexpression and small interfering RNA knock-down. Collectively, we demonstrated that EBV-encoded BARF0 promotes the tumorigenic activity of breast cancer cells through activation of HER2/HER3 signaling cascades.

scholarly journals Epstein-Barr Virus (EBV) Genome and Expression in Breast Cancer Tissue: Effect of EBV Infection of Breast Cancer Cells on Resistance to Paclitaxel (Taxol)

2006 ◽  
Vol 80 (2) ◽  
pp. 845-853 ◽  
Author(s):  
Hratch Arbach ◽  
Viktor Viglasky ◽  
Florence Lefeu ◽  
Jean-Marc Guinebretière ◽  
Vanessa Ramirez ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Breast Cancer Cells ◽  
Epstein Barr Virus ◽  
Breast Cancer Tissue ◽  
Cancer Tissue ◽  
Barr Virus ◽  
Ebv Infection ◽  
Epstein Barr

ABSTRACT The Epstein-Barr virus (EBV) has been detected in subsets of breast cancers. In order to elaborate on these observations, we quantified by real-time PCR (Q-PCR) the EBV genome in biopsy specimens of breast cancer tissue as well as in tumor cells isolated by microdissection. Our findings show that EBV genomes can be detected by Q-PCR in about half of tumor specimens, usually in low copy numbers. However, we also found that the viral load is highly variable from tumor to tumor. Moreover, EBV genomes are heterogeneously distributed in morphologically identical tumor cells, with some clusters of isolated tumor cells containing relatively high genome numbers while other tumor cells isolated from the same specimen may be negative for EBV DNA. Using reverse transcription-PCR, we detected EBV gene transcripts: EBNA-1 in almost all of the EBV-positive tumors and RNA of the EBV oncoprotein LMP-1 in a smaller subset of the tissues analyzed. Moreover, BARF-1 RNA was detected in half of the cases studied. Furthermore, we observed that in vitro EBV infection of breast carcinoma cells confers resistance to paclitaxel (taxol) and provokes overexpression of a multidrug resistance gene (MDR1). Consequently, even if a small number of breast cancer cells are EBV infected, the impact of EBV infection on the efficiency of anticancer treatment might be of importance.

Epstein-Barr virus and breast cancer: Serological study in a high-incidence area of nasopharyngeal carcinoma

2011 ◽  
Vol 309 (2) ◽  
pp. 128-136 ◽  
Author(s):  
Jian-Rong He ◽  
Lu-Ying Tang ◽  
Dan-Dan Yu ◽  
Feng-Xi Su ◽  
Er-Wei Song ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Serological Study ◽  
Barr Virus ◽  
High Incidence ◽  
Epstein Barr ◽  
High Incidence Area

scholarly journals Epstein-Barr virus and breast cancer: Epidemiological and Molecular study on Egyptian and Iraqi women

2012 ◽  
Vol 24 (3) ◽  
pp. 123-131 ◽  
Author(s):  
Abdel-Rahman N. Zekri ◽  
Abeer A. Bahnassy ◽  
Waleed S. Mohamed ◽  
Fatma A. El-Kassem ◽  
Saja J. El-Khalidi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epstein Barr Virus ◽  
Molecular Study ◽  
Barr Virus ◽  
Iraqi Women ◽  
Epstein Barr
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