Impact of Extracorporeal Photopheresis on Blood Parameters of Atopic Dermatitis Patients

AbstractThe pathogenesis of atopic dermatitis (AD) involves complex factors, including gut microbiota and immune modulation, which remain poorly understood. The aim of this study was to restore gut microbiota via fecal microbiota transplantation (FMT) to ameliorate AD in mice. FMT was performed using stool from donor mice. The gut microbiota was characterized via 16S rRNA sequencing and analyzed using Quantitative Insights into Microbial Ecology 2 with the DADA2 plugin. Gut metabolite levels were determined by measuring fecal short-chain fatty acid (SCFA) contents. AD-induced allergic responses were evaluated by analyzing blood parameters (IgE levels and eosinophil percentage, eosinophil count, basophil percentage, and monocyte percentage), the levels of Th1 and Th2 cytokines, dermatitis score, and the number of mast cells in the ileum and skin tissues. Calprotectin level was measured to assess gut inflammation after FMT. FMT resulted in the restoration of gut microbiota to the donor state and increases in the levels of SCFAs as gut metabolites. In addition, FMT restored the Th1/Th2 balance, modulated Tregs through gut microbiota, and reduced IgE levels and the numbers of mast cells, eosinophils, and basophils. FMT is associated with restoration of gut microbiota and immunologic balance (Th1/Th2) along with suppression of AD-induced allergic responses and is thus a potential new therapy for AD.

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Extracorporeal Photopheresis as a Treatment for Patients with Severe, Refractory Atopic Dermatitis

Dermatology ◽

10.1159/000104265 ◽

2007 ◽

Vol 215 (2) ◽

pp. 134-138 ◽

Cited By ~ 27

Author(s):

Michael Sand ◽

Falk G. Bechara ◽

Daniel Sand ◽

Michael Radenhausen ◽

Nordwig S. Tomi ◽

...

Keyword(s):

Atopic Dermatitis ◽

Extracorporeal Photopheresis

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A Retrospective Study of Six Cases of Severe Recalcitrant Atopic Dermatitis Treated with Long-term Extracorporeal Photopheresis

Acta Dermato Venereologica ◽

10.2340/00015555-0952 ◽

2010 ◽

Vol 90 (6) ◽

pp. 635-636 ◽

Cited By ~ 15

Author(s):

KP Hjuler ◽

C Vestergaard ◽

M Deleuran

Keyword(s):

Atopic Dermatitis ◽

Retrospective Study ◽

Extracorporeal Photopheresis

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Cyclosporine and Extracorporeal Photopheresis are Equipotent in Treating Severe Atopic Dermatitis: A Randomized Cross-Over Study Comparing Two Efficient Treatment Modalities

Frontiers in Medicine ◽

10.3389/fmed.2014.00033 ◽

2014 ◽

Vol 1 ◽

Cited By ~ 4

Author(s):

Uffe Koppelhus ◽

Johan Poulsen ◽

Niels Grunnet ◽

Mette SÃ¸ndergaard Deleuran ◽

Erik Obitz

Keyword(s):

Atopic Dermatitis ◽

Treatment Modalities ◽

Severe Atopic Dermatitis ◽

Extracorporeal Photopheresis ◽

Efficient Treatment

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Modern Aspects of Phototherapy for Atopic Dermatitis

Journal of Allergy ◽

10.1155/2012/121797 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Sonja Alexandra Grundmann ◽

Stefan Beissert

Keyword(s):

Atopic Dermatitis ◽

Cytokine Production ◽

Antimicrobial Effect ◽

Inflammatory Cells ◽

Extracorporeal Photopheresis ◽

Therapeutic Modalities ◽

Narrowband Uvb ◽

Proven Efficacy ◽

Atopic Skin

Phototherapy has still great importance in the treatment of atopic dermatitis, though costs, compliance, and long-term risks narrow its relevance. In spite of its long history, up to now, the therapeutic regimes are mostly empirical. Narrowband UVB und UVA1 are the most frequently applied regimens in atopic dermatitis with proven efficacy. However, even for these modalities randomized prospective and controlled studies are still pending. Advances in photoimmunology and molecular biology had demonstrated that phototherapy targets inflammatory cells, alters cytokine production, and has a significant antimicrobial effect within atopic skin. This paper summarizes the current literature on the different regimes of phototherapy and also discusses therapeutic modalities like photochemotherapy and extracorporeal photopheresis. These more complex regimes should be restricted to severe cases of atopic dermatitis, which are refractory to topical treatment.

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Study of the growth, blood parameters and food allergens in Atopic Dermatitis infant under the first year of life in Korea

The FASEB Journal ◽

10.1096/fasebj.20.5.a1058 ◽

2006 ◽

Vol 20 (5) ◽

Author(s):

Eun‐Young Lee ◽

Kyoung‐A Ryu ◽

Jung‐Eun Yim ◽

JW Oh ◽

GW Noh ◽

...

Keyword(s):

Atopic Dermatitis ◽

Blood Parameters ◽

First Year ◽

Food Allergens ◽

First Year Of Life

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THE METHOD FOR PREDICTIVE ASSESSMENT OF THE CONDITION OF PATIENTS WITH ATOPIC DERMATITIS AT DIFFERENT STAGES OF THE DISEASE

Innovative technologies and scientific solutions for industries ◽

10.30837/itssi.2021.16.063 ◽

2021 ◽

pp. 63-71

Author(s):

Oleg Avrunin ◽

Alexey Trubitcin ◽

Viktoriia Klymenko

Keyword(s):

Atopic Dermatitis ◽

Blood Parameters ◽

Color Characteristics ◽

Prognostic Assessment ◽

Discrimination Model ◽

Predictive Assessment ◽

The Subject ◽

Severity Of The Disease ◽

The Given ◽

Degrees Of Severity

The subject of the research is the development of a method for prognostic assessment of the condition of patients with atopic dermatitis at different stages of the disease. The goal of the work is to study the informativeness of immunological indicators and data from dermatoscopic examinations in order to expand the possibilities of prognostic objectification of methods for assessing the condition of patients with atopic dermatitis with varying degrees of severity of the disease. The task of the work includes objectifying the blurring of assessment standards when analyzing the transition from one stage of the disease to another. Methods. The solution to this problem is possible when assessing the possibility of using models of parametric recognition (discrimination) using indicators of immunoglobulins in blood serum and indicators of immunograms, as well as color characteristics of skin areas based on the analysis of dermatoscopic images at various degrees of severity of the disease. Result. In the course of the study, the analysis of the color characteristics of the skin showed that when immunological blood parameters are added to the discrimination model, the probability of an error in making prognostic decisions significantly decreases. Predictive assessment of the condition of a patient with atopic dermatitis only by the color characteristics of the skin makes it possible to control this pathology with a higher degree of probability, which makes it possible to use the digital dermatoscopy method independently for express objectification of the condition of a patient with atopic dermatitis without waiting for the data of immunological analyzes. When a new patient appears, the above indicators are calculated for him and the normalized Euclidean distances to the center of the clusters corresponding to the studied pathologies are calculated. The calculated distances can be ranked and the probabilities of correspondence of the given case to specific pathologies can be determined. Conclusions. The prospect of further work is to substantiate the metrological characteristics of the method to eliminate possible systematic errors associated with the method of obtaining optical information.

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