Sequential Scalp Assessment in Hair Regeneration Therapy Using an Adipose-Derived Stem Cell–Conditioned Medium

2020 ◽  
Vol 46 (6) ◽  
pp. 819-825 ◽  
Author(s):  
Keigo Narita ◽  
Hirotaro Fukuoka ◽  
Takuya Sekiyama ◽  
Hirotaka Suga ◽  
Kiyonori Harii
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Hair Regeneration ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

scholarly journals The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

2020 ◽  
Vol 10 (8) ◽  
pp. 2646
Author(s):  
Keng-Liang Ou ◽  
Yun-Wen Kuo ◽  
Chia-Yu Wu ◽  
Bai-Hung Huang ◽  
Fang-Tzu Pai ◽  
...  
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Hair Follicle ◽  
Conditioned Medium ◽  
Hair Growth ◽  
Hair Regeneration ◽  
Hacat Keratinocyte ◽  
Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

scholarly journals Adipose-derived stem cell conditioned medium accelerates keratinocyte differentiation via the upregulation of miR-24

2015 ◽  
Vol 24 (10) ◽  
pp. 792-793 ◽  
Author(s):  
Gimoon Seo ◽  
Eunhye Oh ◽  
Mihee Yun ◽  
Ji-Young Lee ◽  
Joon Sung Bae ◽  
...  
Keyword(s):  
Stem Cell ◽  
Conditioned Medium ◽  
Keratinocyte Differentiation ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

scholarly journals Protective effects of low-molecular-weight components of adipose stem cell-derived conditioned medium on dry eye syndrome in mice

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuan-Chieh Lee ◽  
Li-Yi Sun ◽  
Jia-Rong Zhang
Keyword(s):  
Molecular Weight ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Dry Eye ◽  
Goblet Cells ◽  
Low Molecular Weight ◽  
Protective Effects ◽  
Corneal Epithelial ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

AbstractThe present study demonstrated the protective effects of low-molecular-weight adipose-derived stem cell-conditioned medium (LADSC-CM) in a mouse model of dry eye syndrome. Mice subjected to desiccating stress and benzalkonium chloride had decreased tear secretion, impaired corneal epithelial tight junction with microvilli, and decreased conjunctival goblet cells. Topical application of adipose-derived stem cell-conditioned medium (ADSC-CM) stimulated lacrimal tear secretion, preserved tight junction and microvilli of the corneal epithelium, and increased the density of goblet cells and MUC16 expression in the conjunctiva. The low-molecular-weight fractions (< 10 kDa and < 3 kDa) of ADSC-CM (LADSC-CM) provided better protections than the > 10 kDa or > 3 kDa fractions of ADSC-CM. In the in vitro study, desiccation for 10 min or hyperosmolarity (490 osmols) for 24 h caused decreased viability of human corneal epithelial cells, which were reversed by LADSC-CM. The active ingredients in the LADSC-CM were lipophobic and stable after heating and lyophilization. Our study demonstrated that LADSC-CM had beneficial effects on experimental dry eye. It is worthy of further exploration for the active ingredient(s) and the mechanism.

scholarly journals Adipose derived stem cell conditioned medium effect on proliferation phase of wound healing in Sprague Dawley rat

2018 ◽  
Vol 26 (4) ◽  
pp. 239-45 ◽  
Author(s):  
Twidy Tarcisia ◽  
Lia Damayanti ◽  
Radiana D. Antarianto ◽  
Yefta Moenadjat ◽  
Jeanne A. Pawitan
Keyword(s):  
Wound Healing ◽  
Stem Cell ◽  
Conditioned Medium ◽  
Healing Process ◽  
Basal Medium ◽  
Medium Effect ◽  
Sprague Dawley ◽  
Collagen Density ◽  
Adipose Derived Stem Cell ◽  
Cell Conditioned Medium

Background: Disintegration of skin tissue can lead to disability and death. Recent studies on wound therapy applied stem cells and adipose derived stem cell conditioned medium (ADSC-CM) to improve wound healing. However, the role of ADSC-CM in wound healing mechanism in terms of angiogenesis, quantity of collagen, and epithelialization is not fully understood. Therefore, this study aimed to analyze the levels of growth factors (VEGF and EGF) in ADSC-CM and histological features of angiogenesis, epithelialization, and collagen density after skin incision in Sprague Dawley rats.Methods: Thirty rats were injured at the back (full thickness wound) and treated topically with ADSC-CM, culture medium, basal medium, and without treatment. Mice were sacrificed on days 3, 7, 14, 21 and 28. After sacrificed, tissue samples were examined microscopically to assess angiogenesis, epithelialization, and collagen density. Concentrations of VEGF and EGF in ADSC-CM were measured by ELISA.Results: Clinically, wound that was treated with ADSC-CM showed improvement in wound healing process. ADSC-CM treated wound showed the highest epithelialization ratio and the fastest wound closure.Conclusion: There were no statistical significant differences between groups that were treated with ADSC-CM and not. However, topical ADSC-CM treated wound revealed a better clinical improvement in epithelialization.

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