Adipose derived stem cell conditioned medium effect on proliferation phase of wound healing in Sprague Dawley rat

Background: Disintegration of skin tissue can lead to disability and death. Recent studies on wound therapy applied stem cells and adipose derived stem cell conditioned medium (ADSC-CM) to improve wound healing. However, the role of ADSC-CM in wound healing mechanism in terms of angiogenesis, quantity of collagen, and epithelialization is not fully understood. Therefore, this study aimed to analyze the levels of growth factors (VEGF and EGF) in ADSC-CM and histological features of angiogenesis, epithelialization, and collagen density after skin incision in Sprague Dawley rats.Methods: Thirty rats were injured at the back (full thickness wound) and treated topically with ADSC-CM, culture medium, basal medium, and without treatment. Mice were sacrificed on days 3, 7, 14, 21 and 28. After sacrificed, tissue samples were examined microscopically to assess angiogenesis, epithelialization, and collagen density. Concentrations of VEGF and EGF in ADSC-CM were measured by ELISA.Results: Clinically, wound that was treated with ADSC-CM showed improvement in wound healing process. ADSC-CM treated wound showed the highest epithelialization ratio and the fastest wound closure.Conclusion: There were no statistical significant differences between groups that were treated with ADSC-CM and not. However, topical ADSC-CM treated wound revealed a better clinical improvement in epithelialization.

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The Effects of Cytokines in Adipose Stem Cell-Conditioned Medium on the Migration and Proliferation of Skin FibroblastsIn Vitro

BioMed Research International ◽

10.1155/2013/578479 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 24

Author(s):

Jiajia Zhao ◽

Li Hu ◽

Jiarong Liu ◽

Niya Gong ◽

Lili Chen

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Conditioned Medium ◽

Healing Process ◽

Dermal Fibroblasts ◽

Wound Healing Process ◽

Adipose Stem Cell ◽

And Migration ◽

Cell Conditioned Medium ◽

Proliferation And Migration

Although adipose stem cell-conditioned medium (ASC-CM) has demonstrated the effect of promoting the cutaneous wound healing, the mechanism for this response on the effector cells (e.g., dermal fibroblasts) during the process remains to be determined. In this study, we aim to investigate the types and contents of cytokines in ASC-CM and the effects of some kinds of common cytokines in ASC-CM, such as EGF, PDGF-AA, VEGF, and bFGF, on dermal fibroblasts proliferation and migration in wound healing process. Results showed that these four cytokines had high concentrations in ASC-CM. The migration of skin fibroblasts could be significantly stimulated by VEGF, bFGF, and PDGF-AA, and the proliferation could be significantly stimulated by bFGF and EGF in ASC-CM. Additionally, ASC-CM had more obvious promoting effect on fibroblasts proliferation and migration than single cytokine. These observations suggested that ASC-CM played an important role in the cutaneous injury partly by the synergistic actions of several cytokines in promoting dermal fibroblasts proliferation and migration, and ASC-CM was more adaptive than each single cytokine to be applied in promoting the wound healing.

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Adipose-Derived Stem Cell Conditioned Medium and Wound Healing: A Systematic Review

Tissue Engineering Part B Reviews ◽

10.1089/ten.teb.2021.0100 ◽

2021 ◽

Author(s):

Bihang Zhang ◽

Yunyan Wu ◽

Masanori Mori ◽

Kotaro Yoshimura

Keyword(s):

Systematic Review ◽

Wound Healing ◽

Stem Cell ◽

Conditioned Medium ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

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The Potential of a Hair Follicle Mesenchymal Stem Cell-Conditioned Medium for Wound Healing and Hair Follicle Regeneration

Applied Sciences ◽

10.3390/app10082646 ◽

2020 ◽

Vol 10 (8) ◽

pp. 2646

Author(s):

Keng-Liang Ou ◽

Yun-Wen Kuo ◽

Chia-Yu Wu ◽

Bai-Hung Huang ◽

Fang-Tzu Pai ◽

...

Keyword(s):

Wound Healing ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Hair Follicle ◽

Conditioned Medium ◽

Hair Growth ◽

Hair Regeneration ◽

Hacat Keratinocyte ◽

Cell Conditioned Medium

The study elucidated the wound healing and hair regeneration properties of a conditioned medium prepared from the culture of human hair follicle mesenchymal stem cells (HFMSCs). The wound-healing effects of mesenchymal stem cell-conditioned medium (MSC-CM) were tested in vitro using scratch assays co-cultured with HaCaT keratinocyte and monitored through optical microscopy. The cell proliferation of HFMSCs and the HaCaT keratinocyte were observed in the presence of different kinds of drugs including UK5099, sodium L-lactate, lactate dehydrogenase-A, MSC-CM, caffeine, and caffeic acid. The hair regeneration properties were investigated in vivo by administrating the MSC-CM solutions to adult B6 mouse models. For quantification, hematoxylin and eosin staining were performed following euthanasia. In vitro results revealed that MSC-CM promotes dermal cell migrations and enhances proliferation of HFMSCs and HaCaT keratinocytes, demonstrating wound-healing properties. Moreover, when the MSC-CM solutions were applied to the shaved mouse skin, a dark area that expanded overtime was seen. Although no hair growth was found, histological analysis proved that a fat layer thickness increment was found under the mouse’s skin, ultimately projecting the formation of new hair growth. MSC-CM promotes the migration and proliferation of dermal keratinocytes that are beneficial for wound healing and hair growth. It is believed that MSC-CM can potentially serve as the basis of alternative therapeutic applications for wound closure and skin regeneration as well as hair growth stimulation and hair loss prevention in alopecia.

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Adipose-derived stem cell-conditioned medium ameliorates antidepression-related behaviors in the mouse model of Alzheimer’s disease

Neuroscience Letters ◽

10.1016/j.neulet.2015.10.023 ◽

2015 ◽

Vol 609 ◽

pp. 53-57 ◽

Cited By ~ 9

Author(s):

Hiromitsu Yamazaki ◽

Yu Jin ◽

Ayako Tsuchiya ◽

Takeshi Kanno ◽

Tomoyuki Nishizaki

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Stem Cell ◽

Mouse Model ◽

Conditioned Medium ◽

Model Of Alzheimer’S Disease ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

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Adipose-derived stem cell conditioned medium (ASC-CM) promotes revascularization of ischemic tissues in a rabbit model of peripheral artery disease

Cryobiology ◽

10.1016/j.cryobiol.2015.10.025 ◽

2015 ◽

Vol 71 (3) ◽

pp. 542

Author(s):

B.H. Johnstone ◽

Jana Jurčíková ◽

Ondrej Lassak ◽

Katerina Vítková ◽

Lubomir Pavliska ◽

...

Keyword(s):

Stem Cell ◽

Conditioned Medium ◽

Peripheral Artery Disease ◽

Rabbit Model ◽

Peripheral Artery ◽

Artery Disease ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

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Sequential Scalp Assessment in Hair Regeneration Therapy Using an Adipose-Derived Stem Cell–Conditioned Medium

Dermatologic Surgery ◽

10.1097/dss.0000000000002128 ◽

2020 ◽

Vol 46 (6) ◽

pp. 819-825 ◽

Cited By ~ 1

Author(s):

Keigo Narita ◽

Hirotaro Fukuoka ◽

Takuya Sekiyama ◽

Hirotaka Suga ◽

Kiyonori Harii

Keyword(s):

Stem Cell ◽

Conditioned Medium ◽

Hair Regeneration ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

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Adipose-derived stem cell conditioned medium accelerates keratinocyte differentiation via the upregulation of miR-24

Experimental Dermatology ◽

10.1111/exd.12754 ◽

2015 ◽

Vol 24 (10) ◽

pp. 792-793 ◽

Cited By ~ 2

Author(s):

Gimoon Seo ◽

Eunhye Oh ◽

Mihee Yun ◽

Ji-Young Lee ◽

Joon Sung Bae ◽

...

Keyword(s):

Stem Cell ◽

Conditioned Medium ◽

Keratinocyte Differentiation ◽

Adipose Derived Stem Cell ◽

Cell Conditioned Medium

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Human Bone Marrow Mesenchymal Stem Cell Conditioned Medium Promotes Wound Healing in Deep Second-Degree Burns in Male Rats

Cells Tissues Organs ◽

10.1159/000501651 ◽

2018 ◽

Vol 206 (6) ◽

pp. 317-329 ◽

Cited By ~ 7

Author(s):

Arefeh Aryan ◽

Mohammad Bayat ◽

Shahin Bonakdar ◽

Soudabeh Taheri ◽

Newsha Haghparast ◽

...

Keyword(s):

Bone Marrow ◽

Wound Healing ◽

Stem Cell ◽

Mesenchymal Stem Cell ◽

Conditioned Medium ◽

Wound Closure ◽

Burn Injury ◽

Human Bone ◽

Male Rats ◽

Cell Conditioned Medium

Burn wound treatment is difficult and one of the most challenging problems in the clinic. Researchers have examined the applications of mesenchymal stem cells as a cell-based therapy for skin regeneration. But the role of human bone marrow mesenchymal stem cell conditioned medium (hBM-MSC-CM) in the treatment of burn injury remains unclear. This research aims at detecting whether hBM-MSC-CM can increase the wound healing of deep second-degree burns in male rats. In this study, 32 adult male rats per each time point were randomly divided into four groups: (1) control group, (2) sham group (DMEM), (3) common treatment group (CT), and (4) conditioned media group (CM). A 3 × 3 cm circular burn was created on the back of the rats. On postsurgical days 7, 15, and 28, the wound closure area of each wound was measured and then the skin samples were removed and analyzed using stereological methods. Wound closure area was significantly increased in the CM and CT groups on the 15th and the 28th day after burn injury compared to the control and DMEM groups. The stereological parameters and immunohistochemistry analysis of the wounds revealed significantly improved healing in the CM group compared to the control and other groups. It is concluded that these findings indicate that hBM-MSC-CM promotes skin wound healing by increasing cell proliferation, regulating collagen synthesis and collagen composition, and inducing angiogenesis at the injury site.

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