Preliminary Investigation of Seeding Mesenchymal Stem Cells on Biodegradable Scaffolds for Vascular Tissue Engineering In Vitro

ASAIO Journal ◽  
2009 ◽  
Vol 55 (6) ◽  
pp. 614-619 ◽  
Author(s):  
Chun-Min Li ◽  
Zhong-Gao Wang ◽  
Yong-Quan Gu ◽  
Jian-De Dong ◽  
Rong-Xin Qiu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Preliminary Investigation ◽  
Vascular Tissue Engineering ◽  
Biodegradable Scaffolds

Bioengineered Teeth from Cultured Rat Tooth Bud Cells

2004 ◽  
Vol 83 (7) ◽  
pp. 523-528 ◽  
Author(s):  
M.T. Duailibi ◽  
S.E. Duailibi ◽  
C.S. Young ◽  
J.D. Bartlett ◽  
J.P. Vacanti ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Engineering Applications ◽  
Adult Rat ◽  
Dental Tissues ◽  
Biodegradable Scaffolds ◽  
Bioengineered Tooth ◽  
Tooth Tissue

The recent bioengineering of complex tooth structures from pig tooth bud tissues suggests the potential for the regeneration of mammalian dental tissues. We have improved tooth bioengineering methods by comparing the utility of cultured rat tooth bud cells obtained from three- to seven-day post-natal (dpn) rats for tooth-tissue-engineering applications. Cell-seeded biodegradable scaffolds were grown in the omenta of adult rat hosts for 12 wks, then harvested. Analyses of 12-week implant tissues demonstrated that dissociated 4-dpn rat tooth bud cells seeded for 1 hr onto PGA or PLGA scaffolds generated bioengineered tooth tissues most reliably. We conclude that tooth-tissue-engineering methods can be used to generate both pig and rat tooth tissues. Furthermore, our ability to bioengineer tooth structures from cultured tooth bud cells suggests that dental epithelial and mesenchymal stem cells can be maintained in vitro for at least 6 days.

Vessel graft fabricated by the on-site differentiation of human mesenchymal stem cells towards vascular cells on vascular extracellular matrix scaffold under mechanical stimulation in a rotary bioreactor

2019 ◽  
Vol 7 (16) ◽  
pp. 2703-2713 ◽  
Author(s):  
Na Li ◽  
Alex P. Rickel ◽  
Hanna J. Sanyour ◽  
Zhongkui Hong
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Mechanical Stimulation ◽  
Vascular Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation ◽  
Vascular Tissue Engineering ◽  
Extracellular Matrix Scaffold

Stem cell differentiation on a decellularized native blood vessel scaffold under mechanical stimulation for vascular tissue engineering.

Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

2010 ◽  
Vol 38 (3) ◽  
pp. 649-657 ◽  
Author(s):  
Eoin D. O’Cearbhaill ◽  
Mary Murphy ◽  
Frank Barry ◽  
Peter E. McHugh ◽  
Valerie Barron
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Vascular Tissue Engineering

Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

Nanomedicine ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 201-214 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Asif Sadiq ◽  
Greeshma Ratheesh ◽  
Sreepathy Sridhar ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Core Shell

scholarly journals Bone marrow–derived mesenchymal stem cells facilitate engineering of long-lasting functional vasculature

Blood ◽  
2008 ◽  
Vol 111 (9) ◽  
pp. 4551-4558 ◽  
Author(s):  
Patrick Au ◽  
Joshua Tam ◽  
Dai Fukumura ◽  
Rakesh K. Jain
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Endothelial Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Perivascular Cells ◽  
Perivascular Cell

Abstract Vascular tissue engineering requires a ready source of endothelial cells and perivascular cells. Here, we evaluated human bone marrow–derived mesenchymal stem cells (hMSCs) for use as vascular progenitor cells in tissue engineering and regenerative medicine. hMSCs expressed a panel of smooth muscle markers in vitro including the cardiac/smooth muscle–specific transcription coactivator, myocardin. Cell-cell contact between endothelial cells and hMSCs up-regulated the transcription of myocardin. hMSCs efficiently stabilized nascent blood vessels in vivo by functioning as perivascular precursor cells. The engineered blood vessels derived from human umbilical cord vein endothelial cells and hMSCs remained stable and functional for more than 130 days in vivo. On the other hand, we could not detect differentiation of hMSCs to endothelial cells in vitro, and hMSCs by themselves could not form conduit for blood flow in vivo. Similar to normal perivascular cells, hMSC-derived perivascular cells contracted in response to endothelin-1 in vivo. In conclusion, hMSCs are perivascular cell precursors and may serve as an attractive source of cells for use in vascular tissue engineering and for the study of perivascular cell differentiation.

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