Behavior of Human Mesenchymal Stem Cells in Fibrin-Based Vascular Tissue Engineering Constructs

2010 ◽  
Vol 38 (3) ◽  
pp. 649-657 ◽  
Author(s):  
Eoin D. O’Cearbhaill ◽  
Mary Murphy ◽  
Frank Barry ◽  
Peter E. McHugh ◽  
Valerie Barron
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Vascular Tissue Engineering

Vessel graft fabricated by the on-site differentiation of human mesenchymal stem cells towards vascular cells on vascular extracellular matrix scaffold under mechanical stimulation in a rotary bioreactor

2019 ◽  
Vol 7 (16) ◽  
pp. 2703-2713 ◽  
Author(s):  
Na Li ◽  
Alex P. Rickel ◽  
Hanna J. Sanyour ◽  
Zhongkui Hong
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Mechanical Stimulation ◽  
Vascular Tissue ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation ◽  
Vascular Tissue Engineering ◽  
Extracellular Matrix Scaffold

Stem cell differentiation on a decellularized native blood vessel scaffold under mechanical stimulation for vascular tissue engineering.

Functionalized core/shell nanofibers for the differentiation of mesenchymal stem cells for vascular tissue engineering

Nanomedicine ◽  
2019 ◽  
Vol 14 (2) ◽  
pp. 201-214 ◽  
Author(s):  
Hariharan Ezhilarasu ◽  
Asif Sadiq ◽  
Greeshma Ratheesh ◽  
Sreepathy Sridhar ◽  
Seeram Ramakrishna ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Vascular Tissue Engineering ◽  
Core Shell

Preliminary Investigation of Seeding Mesenchymal Stem Cells on Biodegradable Scaffolds for Vascular Tissue Engineering In Vitro

ASAIO Journal ◽  
2009 ◽  
Vol 55 (6) ◽  
pp. 614-619 ◽  
Author(s):  
Chun-Min Li ◽  
Zhong-Gao Wang ◽  
Yong-Quan Gu ◽  
Jian-De Dong ◽  
Rong-Xin Qiu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Vascular Tissue ◽  
Preliminary Investigation ◽  
Vascular Tissue Engineering ◽  
Biodegradable Scaffolds

scholarly journals Fluid Flow Mechanical Stimulation-Assisted Cartridge Device for the Osteogenic Differentiation of Human Mesenchymal Stem Cells

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 927
Author(s):  
Ki-Taek Lim ◽  
Dinesh-K. Patel ◽  
Sayan-Deb Dutta ◽  
Keya Ganguly
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Fluid Flow ◽  
Osteogenic Differentiation ◽  
Flow Condition ◽  
Cultured Cells ◽  
Human Mesenchymal Stem Cells ◽  
Proliferation And Differentiation ◽  
Static Conditions

Human mesenchymal stem cells (hMSCs) have the potential to differentiate into different types of mesodermal tissues. In vitro proliferation and differentiation of hMSCs are necessary for bone regeneration in tissue engineering. The present study aimed to design and develop a fluid flow mechanically-assisted cartridge device to enhance the osteogenic differentiation of hMSCs. We used the fluorescence-activated cell-sorting method to analyze the multipotent properties of hMSCs and found that the cultured cells retained their stemness potential. We also evaluated the cell viabilities of the cultured cells via water-soluble tetrazolium salt 1 (WST-1) assay under different rates of flow (0.035, 0.21, and 0.35 mL/min) and static conditions and found that the cell growth rate was approximately 12% higher in the 0.035 mL/min flow condition than the other conditions. Moreover, the cultured cells were healthy and adhered properly to the culture substrate. Enhanced mineralization and alkaline phosphatase activity were also observed under different perfusion conditions compared to the static conditions, indicating that the applied conditions play important roles in the proliferation and differentiation of hMSCs. Furthermore, we determined the expression levels of osteogenesis-related genes, including the runt-related protein 2 (Runx2), collagen type I (Col1), osteopontin (OPN), and osteocalcin (OCN), under various perfusion vis-à-vis static conditions and found that they were significantly affected by the applied conditions. Furthermore, the fluorescence intensities of OCN and OPN osteogenic gene markers were found to be enhanced in the 0.035 mL/min flow condition compared to the control, indicating that it was a suitable condition for osteogenic differentiation. Taken together, the findings of this study reveal that the developed cartridge device promotes the proliferation and differentiation of hMSCs and can potentially be used in the field of tissue engineering.

Magnetic field assisted stem cell differentiation – role of substrate magnetization in osteogenesis

2015 ◽  
Vol 3 (16) ◽  
pp. 3150-3168 ◽  
Author(s):  
Sunil Kumar Boda ◽  
Greeshma Thrivikraman ◽  
Bikramjit Basu
Keyword(s):  
Magnetic Field ◽  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Differentiation ◽  
Bone Tissue Engineering ◽  
Human Mesenchymal Stem Cells ◽  
Stem Cell Differentiation

Substrate magnetization as a tool for modulating the osteogenesis of human mesenchymal stem cells for bone tissue engineering applications.

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