An Evaluation of the Ability of the Probiotic Strain Lactobacillus rhamnosus GG to Eliminate the Gastrointestinal Carrier State of Vancomycin-resistant Enterococci in Colonized Children

2011 ◽  
Vol 45 (10) ◽  
pp. 872-877 ◽  
Author(s):  
Patrycja Szachta ◽  
Iwona Ignyś ◽  
Wojciech Cichy
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Carrier State ◽  
Lactobacillus Rhamnosus Gg ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant

scholarly journals Lactobacillus rhamnosus GG and Biochemical Agents Enrich the Shelf Life of Fresh-Cut Bell Pepper (Capsicum annuum L. var. grossum (L.) Sendt)

Foods ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1252
Author(s):  
Kandasamy Saravanakumar ◽  
Anbazhagan Sathiyaseelan ◽  
Arokia Vijaya Anand Mariadoss ◽  
Ramachandran Chelliah ◽  
Xiaowen Hu ◽  
...  
Keyword(s):  
Bacterial Colonization ◽  
Antioxidant Properties ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Bell Pepper ◽  
Microbial Colonization ◽  
Lactobacillus Rhamnosus Gg ◽  
Combined Application ◽  
Fresh Cut

This work analyzed the individual and combined effects of biochemical additives and probiotic strain Lactobacillus rhamnosus GG on red and yellow fresh-cut bell pepper (R- and Y-FCBP, respectively) stored at two different temperatures (4 °C and 15 °C) for 15 days. The results revealed that the combined application of biochemical additives and L. rhamnosus GG inhibited the colonization of total bacterial counts (25.10%), total Salmonella counts (38.32%), total Listeria counts (23.75%), and total fungal counts (61.90%) in FCBP. Total bacterial colonization was found to be higher in R-FCBP (1188.09 ± 9.25 CFU g−1) than Y-FCBP (863.96 ± 7.21 CFU g−1). The storage at 4 °C was prevented 35.38% of microbial colonization in FCBP. Importantly, the L. rhamnosus GG count remained for up to 12 days. Moreover, the combined inoculation of the biochemical additives and L. rhamnosus GG treatments (T3) maintained the quality of R- and Y-FCBP for up to 12 days at 4 °C without any loss of antioxidant properties. This work reports the successful utilization of L. rhamnosus GG as a preservative agent for maintaining the quality of FCBP by preventing microbial colonization.

scholarly journals Lactobacillus rhamnosus GG Outcompetes Enterococcus faecium via Mucus-Binding Pili: Evidence for a Novel and Heterospecific Probiotic Mechanism

2016 ◽  
Vol 82 (19) ◽  
pp. 5756-5762 ◽  
Author(s):  
Hanne L. P. Tytgat ◽  
François P. Douillard ◽  
Justus Reunanen ◽  
Pia Rasinkangas ◽  
Antoni P. A. Hendrickx ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Enterococcus Faecium ◽  
Sequence Similarity ◽  
Lactobacillus Rhamnosus ◽  
Treatment Strategies ◽  
Carrier State ◽  
Molecular Evidence ◽  
Content Type ◽  
Potential Pathogen ◽  
Vancomycin Resistant

ABSTRACTVancomycin-resistant enterococci (VRE) have become a major nosocomial threat.Enterococcus faeciumis of special concern, as it can easily acquire new antibiotic resistances and is an excellent colonizer of the human intestinal tract. Several clinical studies have explored the potential use of beneficial bacteria to weed out opportunistic pathogens. Specifically, the widely studiedLactobacillus rhamnosusstrain GG has been applied successfully in the context of VRE infections. Here, we provide new insight into the molecular mechanism underlying the effects of this model probiotic on VRE decolonization. Both clinical VRE isolates andL. rhamnosusGG express pili on their cell walls, which are the key modulators of their highly efficient colonization of the intestinal mucosa. We found that one of the VRE pilus clusters shares considerable sequence similarity with the SpaCBA-SrtC1 pilus cluster ofL. rhamnosusGG. Remarkable immunological and functional similarities were discovered between the mucus-binding pili ofL. rhamnosusGG and those of the clinicalE. faeciumstrain E1165, which was characterized at the genome level. Moreover,E. faeciumstrain E1165 bound efficiently to mucus, which may be prevented by the presence of the mucus-binding SpaC protein or antibodies againstL. rhamnosusGG or SpaC. These results present experimental support for a novel probiotic mechanism, in which the mucus-binding pili ofL. rhamnosusGG prevent the binding of a potential pathogen to the host. Hence, we provide a molecular basis for the further exploitation ofL. rhamnosusGG and its pilins for prophylaxis and treatment of VRE infections.IMPORTANCEConcern about vancomycin-resistantEnterococcus faeciumcausing nosocomial infections is rising globally. The arsenal of antibiotic strategies to treat these infections is nearly exhausted, and hence, new treatment strategies are urgently needed. Here, we provide molecular evidence to underpin reports of the successful clinical application ofLactobacillus rhamnosusGG in VRE decolonization strategies. Our results provide support for a new molecular mechanism, in which probiotics can perform competitive exclusion and possibly immune interaction. Moreover, we spur further exploration of the potential of intactL. rhamnosusGG and purified SpaC pilin as prophylactic and curative agents of the VRE carrier state.

scholarly journals Impact of Environmental and Genetic Factors on Biofilm Formation by the Probiotic Strain Lactobacillus rhamnosus GG

2007 ◽  
Vol 73 (21) ◽  
pp. 6768-6775 ◽  
Author(s):  
Sarah Lebeer ◽  
Tine L. A. Verhoeven ◽  
M�nica Perea V�lez ◽  
Jos Vanderleyden ◽  
Sigrid C. J. De Keersmaecker
Keyword(s):  
Biofilm Formation ◽  
Culture Medium ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Lipoteichoic Acid ◽  
Microtiter Plate ◽  
Phenotypic Analysis ◽  
Lactobacillus Rhamnosus Gg ◽  
Abiotic Surfaces

ABSTRACTLactobacillus rhamnosusGG (ATCC 53103) is one of the clinically best-studied probiotic organisms. Moreover,L. rhamnosusGG displays very good in vitro adherence to epithelial cells and mucus. Here, we report thatL. rhamnosusGG is able to form biofilms on abiotic surfaces, in contrast to other strains of theLactobacillus caseigroup tested under the same conditions. Microtiter plate biofilm assays indicated that in vitro biofilm formation byL. rhamnosusGG is strongly modulated by culture medium factors and conditions related to the gastrointestinal environment, including low pH; high osmolarity; and the presence of bile, mucins, and nondigestible polysaccharides. Additionally, phenotypic analysis of mutants affected in exopolysaccharides (wzb), lipoteichoic acid (dltD), and central metabolism (luxS) showed their relative importance in biofilm formation byL. rhamnosusGG.

A Validated Real-Time PCR Method for the Specific Identification of Probiotic Strain Lactobacillus rhamnosus GG (ATCC 53103)

2020 ◽  
Vol 103 (6) ◽  
pp. 1604-1609 ◽  
Author(s):  
Hanan R Shehata ◽  
Steven G Newmaster
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Limit Of Detection ◽  
Health Benefits ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Hypothetical Protein ◽  
Lactobacillus Rhamnosus Gg ◽  
Specific Identification ◽  
Relative Standard

Abstract Background Strain Lactobacillus rhamnosus GG is one of the best-studied and most widely used probiotic strains, with various health benefits. Because probiotic health benefits and safety are strain specific, the availability of a reliable assay for specific identification of Lactobacillus rhamnosus GG is vital to ensure probiotic efficacy. Objective To design and validate a probe-based real-time PCR assay for specific identification of strain Lactobacillus rhamnosus GG. Method Rapid Annotation using Subsystem Technology (RAST) was used to find a unique sequence region in the genome of Lactobacillus rhamnosus GG. A probe-based assay was designed and evaluated for specificity, sensitivity, efficiency, repeatability, and reproducibility. Results RAST identified a unique gene coding for a hypothetical protein in the genome of Lactobacillus rhamnosus GG. The assay successfully amplified all 22 target samples and did not amplify any of the 28 non-target strains, achieving 100% true positive and 0% false positive results. The Limit of Detection (LOD) was determined to be 0.001 ng. Reaction efficiency values, from three dilution series, were 96.4%, 93.3%, and 96.8% with R square values of 0.9974, 0.9981, and 0.9998, respectively. Relative standard deviation (RSD, %) of repeatability was below 1% and RSD of reproducibility was below 4%. Conclusions This Lactobacillus rhamnosus GG specific assay proved to be specific, sensitive, efficient, and reproducible. Since the assay was evaluated on two real-time PCR platforms, including a portable one, the assay can be used for onsite testing throughout the supply chain. Highlights The availability of validated and reliable assays for strain-specific identification plays a vital role in achieving compliance in probiotic products.

scholarly journals Applying sprouts of selected legumes as carriers for Lactobacillus rhamnosus GG – screening studies

2017 ◽  
Vol 113 (4) ◽  
pp. 37-47
Author(s):  
Michał Świeca ◽  
Monika Kordowska-Wiater ◽  
Monika Pytka ◽  
Łukasz Sęczyk ◽  
Urszula Gawlik-Dziki
Keyword(s):  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Quality Parameters ◽  
Probiotic Bacteria ◽  
Animal Nutrition ◽  
Legume Species ◽  
Lactobacillus Rhamnosus Gg ◽  
Inoculation Methods ◽  
Germination Temperature ◽  
Bean Sprouts

Probiotics and prebiotics play an important role in human and animal nutrition. Those research studies were performed to evaluate the potential of using legume sprouts as carriers for probiotic strain of Lactobacillus rhamnosus GG. They determined the effect of legume species, temperature of sprouting, and inoculation methods of seeds or growing sprouts on the survival and/or growth of probiotics. It was found that the count of bacteria in sprouts depended on the germination temperature, inoculation methods as well as on the species of legume used as a carrier. The beans examined (Adzuki and Mung) germinated effectively at a temperature between 25 ÷ 35 ºC. And the lentil sprouted most effectively at 25 ºC. In the case of soy-bean and lentil, the temperature of 35 ºC caused the germination efficiency to decrease. The growth of Lb. rhamnosus GG was reported only in the case of the lentil and soy-bean sprouts obtained from the seeds imbibed in an inoculum and germinated at 25 ºC. The count of probiotic bacteria was 3.1×106 and 7.18×106 CFU per grams of fresh mass, respectively. The sprouts obtained from the bean seeds analyzed did not provide any conditions for probiotic bacteria to survive and grow. The best carrier for the probiotic bacteria studied were the soy-bean sprouts; in their case, after inoculation of seeds and using a suspension of probiotic bacteria, the sprouts obtained at 25 ºC had the best quality parameters.

scholarly journals Mucosal Adhesion Properties of the Probiotic Lactobacillus rhamnosus GG SpaCBA and SpaFED Pilin Subunits

2010 ◽  
Vol 76 (7) ◽  
pp. 2049-2057 ◽  
Author(s):  
Ingemar von Ossowski ◽  
Justus Reunanen ◽  
Reetta Satokari ◽  
Satu Vesterlund ◽  
Matti Kankainen ◽  
...  
Keyword(s):  
Recombinant Proteins ◽  
Binding Capacity ◽  
Lactobacillus Rhamnosus ◽  
Probiotic Strain ◽  
Gene Clusters ◽  
Probiotic Bacterium ◽  
Lactobacillus Rhamnosus Gg ◽  
Adhesion Properties ◽  
Intestinal Mucus ◽  
Pilin Subunit

ABSTRACT Lactobacillus rhamnosus GG is a well-established Gram-positive probiotic strain, whose health-benefiting properties are dependent in part on prolonged residence in the gastrointestinal tract and are likely dictated by adherence to the intestinal mucosa. Previously, we identified two pilus gene clusters (spaCBA and spaFED) in the genome of this probiotic bacterium, each of which contained the predicted genes for three pilin subunits and a single sortase. We also confirmed the presence of SpaCBA pili on the cell surface and attributed an intestinal mucus-binding capacity to one of the pilin subunits (SpaC). Here, we report cloning of the remaining pilin genes (spaA, spaB, spaD, spaE, and spaF) in Escherichia coli, production and purification of the recombinant proteins, and assessment of the adherence of these proteins to human intestinal mucus. Our findings indicate that the SpaB and SpaF pilin subunits also exhibit substantial binding to mucus, which can be inhibited competitively in a dose-related manner. Moreover, the binding between the SpaB pilin subunit and the mucosal substrate appears to operate through electrostatic contacts and is not related to a recognized mucus-binding domain. We conclude from these results that it is conceivable that two pilin subunits (SpaB and SpaC) in the SpaCBA pilus fiber play a role in binding to intestinal mucus, but for the uncharacterized and putative SpaFED pilus fiber only a single pilin subunit (SpaF) is potentially responsible for adhesion to mucus.

Sign in / Sign up

Export Citation Format

Share Document