Reference Genes for Gene Expression Analysis by Real-time Reverse Transcription Polymerase Chain Reaction of Renal Cell Carcinoma

2011 ◽  
Vol 20 (4) ◽  
pp. 212-217 ◽  
Author(s):  
Henriette Bjerregaard ◽  
Shona Pedersen ◽  
Søren Risom Kristensen ◽  
Niels Marcussen
Keyword(s):  
Gene Expression ◽  
Renal Cell Carcinoma ◽  
Polymerase Chain Reaction ◽  
Cell Carcinoma ◽  
Reverse Transcription ◽  
Renal Cell ◽  
Reference Genes ◽  
Gene Expression Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A novel universal probe library quantitative reverse transcription polymerase chain reaction method to profile immunological gene expression in blood of captive beluga whales (Delphinapterusleucas)

2017 ◽  
Author(s):  
Ming-An Tsai ◽  
I-Hua Chen ◽  
Jiann-Hsiung Wang ◽  
Shih-Jen Chou ◽  
Tsung-Hsien Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Immune System ◽  
Reverse Transcription ◽  
Reference Genes ◽  
Amplification Efficiency ◽  
Chain Reaction ◽  
Beluga Whales ◽  
Qrt Pcr ◽  
Polymerase Chain

Cytokines are fundamental for a functioning immune system, and thus, potentially serve as important indicators of animal health. Quantitation of mRNA using quantitative reverse transcription polymerase chain reaction (qRT-PCR) is an established immunological technique. It is particularly suitable for detecting the expression of proteins against which monoclonal antibodies are not available. In this study, we developed a novel probe-based quantitative gene expression assay for immunological assessment of captive beluga whales ( Delphinapterusleucas ) that is one of the most common cetacean species on display in aquariums worldwide. Six immunologically relevant genes (IL-2Rα, -4, -10, -12, TNFα, and IFNγ) were selected for analysis, and two validated housekeeping genes (PGK1 and RPL4) with stable expression were used as reference genes. Sixteen blood samples were obtained from four animals with different health conditions and stored in RNAlater solution. These samples were used for RNA extraction followed by qRT-PCR analysis. Analysis of gene transcripts was performed by relative quantitation using the comparative Cq method with the integration of amplification efficiency and two reference genes. The expression levels of each gene in the samples from clinically healthy animals were normally distributed. Transcript outliers for IL-2Rα, IL-4, IL-12, TNFα, and IFNγ were noticed in four samples collected from two clinically unhealthy animals. This assay has the potential to identify immune system deviation from normal state, which is caused by health problems. Furthermore, knowing the immune status of captive cetaceans could help both trainers and veterinarians in implementing preventive approaches prior to disease onset.

THE EXPRESSION OF NOV AND WT1 IN RENAL CELL CARCINOMA: A QUANTITATIVE REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION ANALYSIS

2005 ◽  
Vol 174 (4 Part 1) ◽  
pp. 1460-1462 ◽  
Author(s):  
ZHIHONG NIU ◽  
MASAKI ITO ◽  
YASUO AWAKURA ◽  
TAKESHI TAKAHASHI ◽  
EIJIRO NAKAMURA ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Polymerase Chain Reaction ◽  
Reverse Transcriptase ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Polymerase Chain Reaction Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A novel universal probe library quantitative reverse transcription polymerase chain reaction method to profile immunological gene expression in blood of captive beluga whales (Delphinapterusleucas)

2017 ◽  
Author(s):  
Ming-An Tsai ◽  
I-Hua Chen ◽  
Jiann-Hsiung Wang ◽  
Shih-Jen Chou ◽  
Tsung-Hsien Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Immune System ◽  
Reverse Transcription ◽  
Reference Genes ◽  
Amplification Efficiency ◽  
Chain Reaction ◽  
Beluga Whales ◽  
Qrt Pcr ◽  
Polymerase Chain

Cytokines are fundamental for a functioning immune system, and thus, potentially serve as important indicators of animal health. Quantitation of mRNA using quantitative reverse transcription polymerase chain reaction (qRT-PCR) is an established immunological technique. It is particularly suitable for detecting the expression of proteins against which monoclonal antibodies are not available. In this study, we developed a novel probe-based quantitative gene expression assay for immunological assessment of captive beluga whales ( Delphinapterusleucas ) that is one of the most common cetacean species on display in aquariums worldwide. Six immunologically relevant genes (IL-2Rα, -4, -10, -12, TNFα, and IFNγ) were selected for analysis, and two validated housekeeping genes (PGK1 and RPL4) with stable expression were used as reference genes. Sixteen blood samples were obtained from four animals with different health conditions and stored in RNAlater solution. These samples were used for RNA extraction followed by qRT-PCR analysis. Analysis of gene transcripts was performed by relative quantitation using the comparative Cq method with the integration of amplification efficiency and two reference genes. The expression levels of each gene in the samples from clinically healthy animals were normally distributed. Transcript outliers for IL-2Rα, IL-4, IL-12, TNFα, and IFNγ were noticed in four samples collected from two clinically unhealthy animals. This assay has the potential to identify immune system deviation from normal state, which is caused by health problems. Furthermore, knowing the immune status of captive cetaceans could help both trainers and veterinarians in implementing preventive approaches prior to disease onset.

Renal cell carcinoma: Allelic loss at chromosome 9 using the fluorescent multiplex–polymerase chain reaction technique

2002 ◽  
Vol 33 (9) ◽  
pp. 910-914 ◽  
Author(s):  
Koji Fukunaga ◽  
Takashi Wada ◽  
Hiroaki Matsumoto ◽  
Satoru Yoshihiro ◽  
Hideyasu Matsuyama ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Polymerase Chain Reaction ◽  
Cell Carcinoma ◽  
Renal Cell ◽  
Multiplex Polymerase Chain Reaction ◽  
Chromosome 9 ◽  
Polymerase Chain Reaction Technique ◽  
Allelic Loss ◽  
Chain Reaction ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document