TINJAUAN PENGALAMAN WANITA YANG SEMBUH DARI COVID-19 DI KABUPATEN ROKAN HULU

Fenomena kasus coronavirus SARS-CoV-2 penyebab COVID-19 pada manusia pertama kali dilaporkan terjadi di kota Wuhan, Tiongkok (Cina) pada awal Desember 2019,Jumlah kasus terkonfirmasi Covid-19 di kabupaten Rokan Hulu pada bulan maret 2020 hingga februari 2021 694 kasus,Wanita yang terkonfirmasi Covid-19 berjumlah 152, sembuh 150 orang dan 2 orang meninggal, adapun tujuan penelitian ini untuk mengetahui bagaimana pengalaman seorang wanita yang positif covid-19 hingga sembuh. Penelitian ini adalah penelitian kualitatif dengan menggunakan desain penelitian Fenomenologi. Dari hasil penelitian diperoleh bahwa penularan yang Covid-19 masing-masing responden berbeda-beda, mulai dari penularan di tempat kerja,kontak dengan orang yang terinfeksi,dan kelelahan.Adapun pemeriksaan yang dilakukan sebelum dinyatakan positif Covid-19 adalah pemeriksaan rapid test dan PCR (polymerase chain reaction). tanda dan gejala yang dirasakan diantaranya seperti demam, batuk kering, Flu, nyeri otot, napas pendek, sakit kepala hingga hilangnya sensasi rasa dan bau (anosmia). Masa penyembuhan Covid-19 isolasi selama kurang lebih 2 minggu dan lebih dari 3-6 minggu. Dan usaha yang dilakukan selama penyembuhan seperti mengosumsi makanan yang bergizi, Meskipun makanan bukan sepenuhnya sebagai penangkal virus, namun gizi yang baik akan lebih menguatkan sistem kekebalan tubuh sehingga bisa memberikan perlindungan ekstra kepada tubuh. olahraga ringan, istirahat yang cukup,mengosumsi obat sesuai kebutuhan dan multivitamin. selalu patuhi protokol kesehatan untuk mengurangi penularan Virus Corona dengan menerapkan 5M yaitu : Mencuci tangan, memakai masker, menjaga jarak,menjauhi kerumunan, dan mengurangi mobilitas.

430. Strategies for Prevention of COVID-19 Transmission in Hospitals

Background Infection control measures against the coronavirus disease 2019 (COVID-19) within a hospital often rely on expert experience and intuition due to the lack of clear guidelines. This study surveyed current strategies for the prevention of the spread of COVID-19 in medical institutions. Methods Upon systematic review of the guidelines at the national level, 14 key topics were selected. Six hospitals were provided an open survey that assessed their responses to these topics between August 11 and 25, 2020. Using these data, an online questionnaire was developed and sent to the infection control teams of 46 hospitals in South Korea. The survey was conducted between January 31, 2021, and February 20, 2021. Results All 46 hospitals responded to the survey, and 24 hospitals (52.2%) had treated 100 or more cases of COVID-19. All hospitals operated screening clinics, and the criteria were respiratory symptoms (100%), fever (97.8%), and epidemiological association (93.5%). It was found that 89.1% (41/46) of hospitals allowed symptomatic patients to visit their general outpatient clinics if fever or respiratory symptoms were not associated with COVID-19. Most hospitals (87.2%; 34/39) conducted polymerase chain reaction (PCR) tests for all hospitalized patients. Moreover, 76.1% (35/46) of hospitals implemented preemptive isolation policies for hospitalized patients, of which 97.1% (34/35) were released from isolation after a single negative PCR test. A little over half of the hospitals (58.7%; 27/46) treated patients that met the national criteria for release from isolation but consistently had positive PCR results. Of these hospitals, 63% (17/27) used N95/KF94 masks, and 40.7% (11/27) used surgical masks without other personal protective equipment for treating them. Most hospitals (76.9%; 20/26) accommodated them in shared rooms when the cycle threshold value of the PCR test was more than a certain value (34.6%; 9/26), or after a certain period that satisfied the national criteria (26.9%; 7/26). Finally, 76.1% (35/46) of hospitals performed emergency procedures or operations on suspected patients. Table 1. Screening and selective treatment policy to prevent COVID-19 patients from entering the hospital Note Values are presented as number (%) Abbreviations: COVID-19, coronavirus disease 2019; PCR, polymerase chain reaction 1 This question requested the respondent to select multiple items. 2 Suspected cases of COVID-19 include fever, respiratory symptoms, and epidemiological associations with COVID-19 patients. Note Values are presented as number (%) Abbreviations: COVID-19, coronavirus disease 2019; PCR, polymerase chain reaction; PAPR, powered air-purifying respirator; Ct, cycle threshold 1 This question requested the respondent to select multiple items. 2 It includes infectious diseases, pulmonology, and the infection control and prevention office. 3 One hospital that wrote a non-categorical answer for the question was excluded. The hospital made a decision after discussing it with an infectious diseases specialist. Conclusion Various guidelines were being applied by each medical institution, but there was a lack of an explicit set of national guidelines to support them. Disclosures All Authors: No reported disclosures

Metodologia molecular de identificação de proteínas Cry em bioinseticidas através de PCR (Polymerase Chain Reaction) / Molecular methodology of identification of Cry proteins in bioinsecticides through PCR (Polymerase Chain Reaction)

Bacillus thuringiensis, apresenta atividade tóxica contra espécies das ordens Lepidoptera, Diptera, Hymenoptera e Coleoptera, dentre outras. Dentre as principais características das toxinas produzidas pelo B. thuringiensis tem-se a especificidade aos insetos e biodegradação, tornando a utilização deste microrganismo viável economicamente e ecologicamente, uma vez que evita a contaminação do meio ambiente, e adicionalmente, preserva os inimigos naturais. De acordo com a literatura especializada, estirpes de B. thuringiensis apresentam grande variabilidade genética e neste sentido a utilização da PCR (Polymerase Chain Reaction) apresenta grande destaque, pois auxilia na identificação e caracterização de genes codificadores de δ-endotoxinas, direcionando os trabalhos de bioensaios. Desta forma, o presente trabalho tem como objetivo desenvolver uma metodologia de identificação das principais proteínas (Cry) que compõem os produtos comerciais. Para isso foram selecionados dois produtos comerciais (P1 e P2). Cerca de 1 ml de cada produto foi depositado em placas de petri contendo meio de cultura, e espalhados com uma alça de Drigalski, estas placas foram acondicionadas a temperatura de 28°C por um período de 3 dias para crescimento das colônias bacterianas. Em seguida, realizou-se a obtenção de DNA para identificação das proteínas Cry. Seis proteínas Cry de B. thuringiensis (Cry1Aa, Cry1Ab, Cry1Ac, Cry1C, Cry1D e Cry1F) foram selecionadas para identificação nos bioinseticidas escolhidos. Quando submetidos ao teste com o primer geral Gray-cry1, o qual confirma a presença de genes Cry1, somente o P1 apresentou amplificação molecular, ou seja, presença de proteínas Cry em sua formulação. Diferentemente, de P1, P2 não demostrou presença de nenhuma banda em gel de agarose, o que nos permite concluir que não há presença de proteínas Cry, mesmo estas estando teoricamente presentes na formulação de P2. Este dado é de extrema importância uma vez que molecularmente foi possível comprovar a formulação proteica do bioinseticida P1, pois o perfil molecular foi condizente com a presença/ausência de bandas referentes as proteínas do produto. Os resultados deste trabalho vêm somar com predição de resultados a campo no que diz respeito a efetividade do bioinseticida.

Isolation and Identification of Biosurfactant Producing Bacteria from Slaughterhouse Wastewater

The slaughterhouse wastewater is rich of oil and fat and it may inhabit by biosurfactant producing bacteria. Isolation of the bacteria is needed in order to find out a biosurfactant producing bacteria that can be used to maintain the oil and fat polluted area through bioremediation process. To isolate and identify the biosurfactant-producing bacteria, a study has been conducted on July to September 2020. The samples were obtained from slaughterhouse wastewater at Cipta Karya street Pekanbaru, Riau Province and samplings were conducted three times. The isolation was conducted by TSB (Tryptic Soy Broth) and incubated for 24 hours. Then the bacterial samples were planted by TSA (Tryptic Soy Agar) and isolated for 24 hours. Then, it was identified using biochemical and morphological tests were carried out to find out the types of bacteria. The Emulsification Index was then calculated by using TSB (Tryptic Soy Broth) and kerosene. Results shown that the emulsification index were Flavobacterium 53%, Agrobacterium 53%, Serratia 60%, Salmonella 52. 5%, Salmonella 52.4%, Proteus 65.7%, Clostridium 62% and Aeromonas 59.5%. As Proteus is shown the highest Emulsification Index, it was check using a PCR (Polymerase Chain Reaction) and it is proved that the species is Proteus vulgaris

Reestructuración del funcionamiento de la Unidad de Hospitalización a Domicilio del Hospital Universitario Germans Trias i Pujol para el manejo de pacientes COVID y no COVID ingresados durante la pandemia del COVID-19 en España

Introducción: La Unidad de Hospitalización a Domicilio del Hospital Universitari Germans Trias i Pujol, ante la grave situación sanitaria generada por el COVID-19 optó por varias estrategias para la atención de los pacientes ingresados con o sin diagnóstico de COVID optimizando los recursos de atención sanitaria.Método: Estudio descriptivo sobre la reorganización de la Unidad de Hospitalización a Domicilio (UHAD) durante la pandemia del COVID-19 en el período comprendido entre el 14 de Marzo y 31 de Mayo del 2020. Una ampliación del número de camas virtuales, así como de los turnos e incorporación de personal sanitario (médico/enfermeros) fue necesario, activándose paralelamente 2 plataformas de telemedicina para monitorización y contacto con los pacientes (COVIDApp para los pacientes COVID y Revita para los pacientes no COVID).Resultados: Un total de 781 pacientes referidos del área de hospitalización, urgencias y atención primaria fueron incluidos, 584 (74,8%) ingresados con diagnóstico de COVID-19 (por PCR = polymerase chain reaction) y 197 (25,2%) pacientes ingresados por otras patologías (no-COVID) provenientes de la zona Metropolitana Nord de Barcelona y Maresme. Un 24,6% de los pacientes no-COVID y un 2,5% de los pacientes COVID eran pacientes crónicos complejos. El porcentaje de reingreso hospitalario fue mayor en los pacientes no-COVID (11.6%) que en los pacientes COVID (4,28%). El porcentaje de altas de la UHAD aumentó hasta un 35,34%. Ambas plataformas permitieron realizar seguimiento estrecho de los pacientes.Conclusiones: La pandemia del COVID-19 ha remarcado la necesidad de optimizar y reestructurar los recursos del sistema sanitario, siendo las plataformas de Telemedicina COVIDApp y Revita de ayuda como herramientas innovadoras.

Persyaratan Uji Tes PCR atau Rapid Test untuk Transportasi Umum Dalam Perspektif Ketatanegaraan

Gugus Tugas Percepatan Penanganan Covid-19 dibentuk melalui Keppres No. 7 Tahun 2020 (sebagaimana diubah melalui Keppres No. 9 Tahun 2020). Gugus Tugas telah mengeluarkan surat edaran yang mengatur uji tes PCR (Polymerase Chain Reaction) dan rapid test sebagai syarat untuk menggunakan transportasi umum. Padahal, Keppres a quo tidak memberikan kewenangan kepada Gugus Tugas untuk mengeluarkan suatu peraturan. Penulisan jurnal ini menggunakan metode penelitian hukum normatif, yaitu berbasis pada analisis terhadap norma dalam hukum positif. Gugus Tugas berkedudukan sebagai lembaga negara bantu (state auxiliary organ) yang bersifat koordinasi antar kementerian atau lembaga negara lainnya dan dibentuk melalui Keputusan Presiden. Syarat uji tes PCR dan rapid test perjalanan orang dengan transportasi umum batal demi hukum dan tidak memiliki kekuatan hukum mengikat karena: 1) Gugus Tugas sebagai state auxiliary organ dapat meminta kepada pemerintah untuk mengeluarkan suatu peraturan yang bersifat regeling sesuai dengan jenis dan hierarki peraturan perundang-undangan yang berlaku dalam rangka percepatan penanganan Covid-19; 2) Gugus Tugas tidak memiliki legitimasi ataupun delegasi yang bersumber dari undang-undang untuk mengeluarkan suatu peraturan yang mengikat ke luar dan berlaku umum; 3) Surat Edaran Gugus Tugas No. 9 Tahun 2020 yang bersifat mengatur ke luar tidak mencerminkan asas pembentukan peraturan perundang-undangan yaitu kesesuaian materi muatan dan kejelasan tujuan.

First case of an exceptional Zona of the popliteal fossa

Le Zona est une infection virale aiguë, sporadique et douloureuse chez les personnes âgées, causée par la réactivation du virus de la varicelle-zona latent. C’est une affection dermatologique fréquente qui affecte jusqu'à 20 % de la population, le plus souvent impliquant les dermatomes thoraciques et faciaux avec des lésions sacrées. L'herpès zoster affectant le creux poplité est une pathologie rare. La nature ambiguë de cette condition crée un dilemme diagnostique. Nous rapportons l’observation d’une patiente âgée ayant présenté une localisation exceptionnelle du zona : le creux poplité. C’est le premier cas décrit du zona du creux poplité confirmé par la PCR (Polymerase Chain Reaction) avec des caractéristiques distinctives secondaires chez une femme âgée.

Polymerase chain reaction: a creative review

In molecular biology, a scientific technique PCR (polymerase chain reaction) is used to generate thousands to millions of copies of a single particular DNA sequences to amplify a single or few copies of a piece of DNA across several orders of magnitude. For multiple applications, PCR is an ordinary and often vital practice used in medicinal and biological research labs and is used for diagnosis and investigation of multiple diseases. In PCR mainly three major steps are involved. Denaturation, annealing, and extension. PCR can be used to detect not only the human genome but also the genome of viruses and bacteria. PCR is especially useful in forensic laborites because a very small amount of original DNA is required. In the development of cancer, genes have been implicated through PCR

Seleção de primers polimórficos para estudo de diversidade genética em cactáceas

A família Cactaceae está distribuída principalmente nas Américas, apresentam uma grande importância econômica, fornecendo recursos energéticos para animais polinizadores e dispersores, podendo ser utilizadas na alimentação animal e humana, possui um grande potencial na medicina tradicional e no paisagismo. O objetivo do presente estudo foi selecionar indicadores e padronizar reações de PCR (Polymerase Chain Reaction) para analisar ISSR (Inter Simple Sequence Repeats) em estudos de variabilidade genética de Cactaceae. Foram testados 14 indicadores de ISSR com temperatura variando de 48° a 52°C, em espécies de Tacinga, e destes, 8 foram selecionados por serem polimórficos: ISSR-808, ISSR-827, ISSR-842, ISSR-845, ISSR-853, ISSR-857 ISSR-880 e ISSR-888. O número médio de sequências amplificadas por indicador foi de 11,5 bandas, com destaque para o indicador ISSR-827, que produziu 15 bandas, enquanto os indicadores ISSR-845 e ISSR-853 produziram apenas 8 bandas. Os 8 indicadores selecionados no presente estudo possibilitaram a diferenciação genética, sendo eficientes e indicando um bom nível de polimorfismo entre as espécies analisadas, dessa forma, poderão ser utilizados em futuros trabalhos para estimar a divergência genética em nível molecular em espécies da família Cactaceae.