Freeze dried platelets promote clot formation, rescue thrombin induced endothelial cell permeability, and decrease pulmonary vascular leak in a murine model of hemorrhagic shock

2020 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Alpa Trivedi ◽  
Daniel R. Potter ◽  
Byron Y. Miyazawa ◽  
Maximillian Lin ◽  
Lindsay R. Vivona ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Hemorrhagic Shock ◽  
Murine Model ◽  
Cell Permeability ◽  
Clot Formation ◽  
Vascular Leak ◽  
Freeze Dried ◽  
Endothelial Cell Permeability

Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP

1998 ◽  
Vol 275 (2) ◽  
pp. L203-L222 ◽  
Author(s):  
Timothy M. Moore ◽  
Paul M. Chetham ◽  
John J. Kelly ◽  
Troy Stevens
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Intracellular Signaling ◽  
Endothelial Permeability ◽  
Cell Permeability ◽  
Gap Formation ◽  
Pulmonary Endothelium ◽  
Intracellular Signals ◽  
Endothelial Cell Permeability ◽  
Cell Cell

Pulmonary endothelium forms a semiselective barrier that regulates fluid balance and leukocyte trafficking. During the course of lung inflammation, neurohumoral mediators and oxidants act on endothelial cells to induce intercellular gaps permissive for transudation of proteinaceous fluid from blood into the interstitium. Intracellular signals activated by neurohumoral mediators and oxidants that evoke intercellular gap formation are incompletely understood. Cytosolic Ca2+ concentration ([Ca2+]i) and cAMP are two signals that importantly dictate cell-cell apposition. Although increased [Ca2+]ipromotes disruption of the macrovascular endothelial cell barrier, increased cAMP enhances endothelial barrier function. Furthermore, during the course of inflammation, elevated endothelial cell [Ca2+]idecreases cAMP to facilitate intercellular gap formation. Given the significance of both [Ca2+]iand cAMP in mediating cell-cell apposition, this review addresses potential sites of cross talk between these two intracellular signaling pathways. Emerging data also indicate that endothelial cells derived from different vascular sites within the pulmonary circulation exhibit distinct sensitivities to permeability-inducing stimuli; that is, elevated [Ca2+]ipromotes macrovascular but not microvascular barrier disruption. Thus this review also considers the roles of [Ca2+]iand cAMP in mediating site-specific alterations in endothelial permeability.

Novel infection of pericytes by Andes virus enhances endothelial cell permeability

2021 ◽  
Vol 306 ◽  
pp. 198584
Author(s):  
Ramon D. Perez ◽  
Elena E. Gorbonova ◽  
Erich R. Mackow
Keyword(s):  
Endothelial Cell ◽  
Cell Permeability ◽  
Andes Virus ◽  
Endothelial Cell Permeability

Neoplastic and pharmacological influence on the permeability of an in vitro blood-brain barrier

1995 ◽  
Vol 82 (6) ◽  
pp. 1053-1058 ◽  
Author(s):  
Paul A. Grabb ◽  
Mark R. Gilbert
Keyword(s):  
Endothelial Cell ◽  
Rat Brain ◽  
Phospholipase A ◽  
Cell Permeability ◽  
Capillary Endothelium ◽  
Brain Capillary ◽  
Content Type ◽  
Endothelial Cell Permeability ◽  
Fine Print

✓ The authors investigated the effects of glioma cells and pharmacological agents on the permeability of an in vitro blood-brain barrier (BBB) to determine the following: 1) whether malignant glia increase endothelial cell permeability; 2) how glucocorticoids affect endothelial cell permeability in the presence and absence of malignant glia; and 3) whether inhibiting phospholipase A2, the enzyme that releases arachidonic acid from membrane phospholipids, would reduce any malignant glioma—induced increase in endothelial cell permeability. Primary cultures of rat brain capillary endothelium were grown on porous membranes; below the membrane, C6, 9L rat glioma, T98G human glioblastoma, or no cells (control) were cocultured. Dexamethasone (0.1 µM), bromophenacyl bromide (1.0 µM), a phospholipase A2 inhibitor, or nothing was added to culture media 72 hours prior to assaying the rat brain capillary endothelium permeability. Permeability was measured as the flux of radiolabeled sucrose across the rat brain capillary endothelium monolayer and then calculated as an effective permeability coefficient (Pe). When neither dexamethasone nor bromophenacyl bromide was present, C6 cells reduced the Pe significantly (p < 0.05), whereas 9L and T98G cells increased Pe significantly (p < 0.05) relative to rat brain capillary endothelium only (control). Dexamethasone reduced Pe significantly for all cell preparations (p < 0.05). The 9L and T98G cell preparations coincubated with dexamethasone had the lowest Pe of all cell preparations. The Pe was not affected in any cell preparation by coincubation with bromophenacyl bromide (p > 0.45). These in vitro BBB experiments showed that: 1) malignant glia, such as 9L and T98G cells, increase Pe whereas C6 cells probably provide an astrocytic influence by reducing Pe; 2) dexamethasone provided significant BBB “tightening” effects both in the presence and absence of glioma cells; 3) the in vivo BBB is actively made more permeable by malignant glia and not simply because of a lack of astrocytic induction; 4) tumor or endothelial phospholipase A2 activity is probably not responsible for glioma-induced increased in BBB permeability; and 5) this model is useful for testing potential agents for BBB protection and for studying the pathophysiology of tumor-induced BBB disruption.

scholarly journals ETS-related Gene (ERG) Controls Endothelial Cell Permeability via Transcriptional Regulation of the Claudin 5 (CLDN5) Gene

2012 ◽  
Vol 287 (9) ◽  
pp. 6582-6591 ◽  
Author(s):  
Lei Yuan ◽  
Alexandra Le Bras ◽  
Anastasia Sacharidou ◽  
Kiyoshi Itagaki ◽  
Yumei Zhan ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Endothelial Cell ◽  
Cell Permeability ◽  
Related Gene ◽  
Endothelial Cell Permeability

scholarly journals Regulation of endothelial cell permeability by platelet-derived extracellular vesicles

2019 ◽  
Vol 86 (6) ◽  
pp. 931-942 ◽  
Author(s):  
Byron Miyazawa ◽  
Alpa Trivedi ◽  
Padma Priya Togarrati ◽  
Daniel Potter ◽  
Gyulnar Baimukanova ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Extracellular Vesicles ◽  
Cell Permeability ◽  
Endothelial Cell Permeability
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