Feasibility of Diagnosing Subclinical Renal Allograft Rejection in Children By Whole Blood Gene Expression Analysis

2008 ◽  
Vol 86 (9) ◽  
pp. 1222-1228 ◽  
Author(s):  
Noora Alakulppi ◽  
Paula Seikku ◽  
Taina Jaatinen ◽  
Christer Holmberg ◽  
Jarmo Laine
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Allograft Rejection ◽  
Gene Expression Analysis ◽  
Renal Allograft ◽  
Blood Gene Expression ◽  
Renal Allograft Rejection

scholarly journals Longitudinal Analysis of Whole Blood Transcriptomes to Explore Molecular Signatures Associated with Acute Renal Allograft Rejection

2014 ◽  
Vol 8 ◽  
pp. BBI.S13376 ◽  
Author(s):  
Heesun Shin ◽  
Oliver GÜnther ◽  
Zsuzsanna Hollander ◽  
Janet E. Wilson-Mcmanus ◽  
Raymond T. Ng ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acute Rejection ◽  
Whole Blood ◽  
Allograft Rejection ◽  
Time Course ◽  
Renal Allograft ◽  
Acute Rejection Episode ◽  
Renal Allograft Rejection ◽  
Expression Signature ◽  
Acute Renal Allograft Rejection

In this study, we explored a time course of peripheral whole blood transcriptomes from kidney transplantation patients who either experienced an acute rejection episode or did not in order to better delineate the immunological and biological processes measureable in blood leukocytes that are associated with acute renal allograft rejection. Using microarrays, we generated gene expression data from 24 acute rejectors and 24 nonrejectors. We filtered the data to obtain the most unambiguous and robustly expressing probe sets and selected a subset of patients with the clearest phenotype. We then performed a data-driven exploratory analysis using data reduction and differential gene expression analysis tools in order to reveal gene expression signatures associated with acute allograft rejection. Using a template-matching algorithm, we then expanded our analysis to include time course data, identifying genes whose expression is modulated leading up to acute rejection. We have identified molecular phenotypes associated with acute renal allograft rejection, including a significantly upregulated signature of neutrophil activation and accumulation following transplant surgery that is common to both acute rejectors and nonrejectors. Our analysis shows that this expression signature appears to stabilize over time in nonrejectors but persists in patients who go on to reject the transplanted organ. In addition, we describe an expression signature characteristic of lymphocyte activity and proliferation. This lymphocyte signature is significantly downregulated in both acute rejectors and nonrejectors following surgery; however, patients who go on to reject the organ show a persistent downregulation of this signature relative to the neutrophil signature.

scholarly journals Changes in gene expression at the CD160 locus in the whole blood of sepsis patients.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Differentially Expressed ◽  
Blood Gene Expression ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Sepsis is a leading cause of mortality (1). We mined published datasets from the whole blood of patients with sepsis to identify differentially expressed genes in the septic state (2, 3). We found changes in CD160 expression as among the most significant quantitative differences in sepsis whole blood gene expression. Analysis of a separate dataset (4) demonstrated significant repression of a long non-coding RNA produced at the CD160 locus in the blood of patients with sepsis. In the datasets we analyzed, changes in coding and non-coding gene expression at the CD160 locus were among the most significant changes in gene expression in the blood of patients with sepsis.

scholarly journals Coding and non-coding RNAs transcribed at the RORA locus (ROR𝛂) are differentially expressed in the whole blood of sepsis patients.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Differentially Expressed ◽  
Blood Gene Expression ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Sepsis is a leading cause of mortality (1). We mined published datasets from the whole blood of patients with sepsis to identify differentially expressed genes in the septic state (2, 3). We found changes in RORA expression as among the most significant quantitative differences in sepsis whole blood gene expression. Analysis of a separate dataset (4) demonstrated significant repression of a long non-coding RNA produced at the RORA locus in the blood of patients with sepsis.

scholarly journals Gene Expression Analysis of the Effect of Ischemic Infarction in Whole Blood

2017 ◽  
Vol 18 (11) ◽  
pp. 2335 ◽  
Author(s):  
Ayako Takuma ◽  
Arata Abe ◽  
Yoshikazu Saito ◽  
Chikako Nito ◽  
Masayuki Ueda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Ischemic Infarction

scholarly journals Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Crystal Jaing ◽  
Raymond R. R. Rowland ◽  
Jonathan E. Allen ◽  
Andrea Certoma ◽  
James B. Thissen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
African Swine Fever ◽  
High Pathogenic

Effects of Sample Timing and Treatment on Gene Expression in Early Acute Renal Allograft Rejection

2011 ◽  
Vol 91 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Oliver P. Günther ◽  
David Lin ◽  
Robert F. Balshaw ◽  
Raymond T. Ng ◽  
Zsuzsanna Hollander ◽  
...  
Keyword(s):  
Gene Expression ◽  
Allograft Rejection ◽  
Renal Allograft ◽  
Renal Allograft Rejection ◽  
Acute Renal Allograft Rejection

36-OR: Prospective immune monitoring of perforin and granzyme B gene expression in the peripheral blood detects renal allograft rejection

2007 ◽  
Vol 68 (1) ◽  
pp. S118
Author(s):  
Q. Zhang ◽  
J.L. Veale ◽  
D.W. Gjertson ◽  
B. Suphamai ◽  
P.T.T. Pham ◽  
...  
Keyword(s):  
Gene Expression ◽  
Peripheral Blood ◽  
Allograft Rejection ◽  
Renal Allograft ◽  
Granzyme B ◽  
Immune Monitoring ◽  
Renal Allograft Rejection
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