Whole blood gene expression analysis in idiopathic infantile hypercalcemia due to compound heterozygous mutation in theCYP24A1gene in an Austrian 4-month-old boy and his family

2015 ◽  
Author(s):  
Daniela Hofer ◽  
Verena Zachhuber ◽  
Lisa Lindheim ◽  
Julia Munzker ◽  
Olivia Trummer ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Compound Heterozygous Mutation ◽  
Heterozygous Mutation ◽  
Compound Heterozygous ◽  
Blood Gene Expression ◽  
Idiopathic Infantile Hypercalcemia

Feasibility of Diagnosing Subclinical Renal Allograft Rejection in Children By Whole Blood Gene Expression Analysis

2008 ◽  
Vol 86 (9) ◽  
pp. 1222-1228 ◽  
Author(s):  
Noora Alakulppi ◽  
Paula Seikku ◽  
Taina Jaatinen ◽  
Christer Holmberg ◽  
Jarmo Laine
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Allograft Rejection ◽  
Gene Expression Analysis ◽  
Renal Allograft ◽  
Blood Gene Expression ◽  
Renal Allograft Rejection

scholarly journals Changes in gene expression at the CD160 locus in the whole blood of sepsis patients.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Differentially Expressed ◽  
Blood Gene Expression ◽  
Non Coding Rna ◽  
Long Non Coding Rna

Sepsis is a leading cause of mortality (1). We mined published datasets from the whole blood of patients with sepsis to identify differentially expressed genes in the septic state (2, 3). We found changes in CD160 expression as among the most significant quantitative differences in sepsis whole blood gene expression. Analysis of a separate dataset (4) demonstrated significant repression of a long non-coding RNA produced at the CD160 locus in the blood of patients with sepsis. In the datasets we analyzed, changes in coding and non-coding gene expression at the CD160 locus were among the most significant changes in gene expression in the blood of patients with sepsis.

scholarly journals Coding and non-coding RNAs transcribed at the RORA locus (ROR𝛂) are differentially expressed in the whole blood of sepsis patients.

2020 ◽  
Author(s):  
Shahan Mamoor
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Differentially Expressed ◽  
Blood Gene Expression ◽  
Non Coding Rna ◽  
Non Coding Rnas ◽  
Long Non Coding Rna

Sepsis is a leading cause of mortality (1). We mined published datasets from the whole blood of patients with sepsis to identify differentially expressed genes in the septic state (2, 3). We found changes in RORA expression as among the most significant quantitative differences in sepsis whole blood gene expression. Analysis of a separate dataset (4) demonstrated significant repression of a long non-coding RNA produced at the RORA locus in the blood of patients with sepsis.

scholarly journals Gene Expression Analysis of the Effect of Ischemic Infarction in Whole Blood

2017 ◽  
Vol 18 (11) ◽  
pp. 2335 ◽  
Author(s):  
Ayako Takuma ◽  
Arata Abe ◽  
Yoshikazu Saito ◽  
Chikako Nito ◽  
Masayuki Ueda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
Ischemic Infarction

scholarly journals Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Crystal Jaing ◽  
Raymond R. R. Rowland ◽  
Jonathan E. Allen ◽  
Andrea Certoma ◽  
James B. Thissen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Whole Blood ◽  
Gene Expression Analysis ◽  
African Swine Fever ◽  
High Pathogenic

PERIPHERAL BLOOD GENE EXPRESSION ANALYSIS IN SMALL BOWEL TRANSPLANTATION

2010 ◽  
Vol 90 ◽  
pp. 1021
Author(s):  
P. tryphonopoulos ◽  
V. Andreev ◽  
P. Ruiz ◽  
A. Volsky ◽  
E. Island ◽  
...  
Keyword(s):  
Gene Expression ◽  
Small Bowel ◽  
Expression Analysis ◽  
Peripheral Blood ◽  
Gene Expression Analysis ◽  
Small Bowel Transplantation ◽  
Blood Gene Expression ◽  
Peripheral Blood Gene Expression

scholarly journals Sensitive and Quantitative Measurement of Gene Expression Directly from a Small Amount of Whole Blood

2006 ◽  
Vol 52 (7) ◽  
pp. 1294-1302 ◽  
Author(s):  
Zhi Zheng ◽  
Yuling Luo ◽  
Gary K McMaster
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Reverse Transcription ◽  
Whole Blood ◽  
Quantitative Measurement ◽  
Gene Expression Analysis ◽  
Rna Isolation ◽  
Multiplex Assay ◽  
Quality Data ◽  
Branched Dna

Abstract Background: Accurate and precise quantification of mRNA in whole blood is made difficult by gene expression changes during blood processing, and by variations and biases introduced by sample preparations. We sought to develop a quantitative whole-blood mRNA assay that eliminates blood purification, RNA isolation, reverse transcription, and target amplification while providing high-quality data in an easy assay format. Methods: We performed single- and multiplex gene expression analysis with multiple hybridization probes to capture mRNA directly from blood lysate and used branched DNA to amplify the signal. The 96-well plate singleplex assay uses chemiluminescence detection, and the multiplex assay combines Luminex-encoded beads with fluorescent detection. Results: The single- and multiplex assays could quantitatively measure as few as 6000 and 24 000 mRNA target molecules (0.01 and 0.04 amoles), respectively, in up to 25 μL of whole blood. Both formats had CVs <10% and dynamic ranges of 3–4 logs. Assay sensitivities allowed quantitative measurement of gene expression in the minority of cells in whole blood. The signals from whole-blood lysate correlated well with signals from purified RNA of the same sample, and absolute mRNA quantification results from the assay were similar to those obtained by quantitative reverse transcription-PCR. Both single- and multiplex assay formats were compatible with common anticoagulants and PAXgene-treated samples; however, PAXgene preparations induced expression of known antiapoptotic genes in whole blood. Conclusions: Both the singleplex and the multiplex branched DNA assays can quantitatively measure mRNA expression directly from small volumes of whole blood. The assay offers an alternative to current technologies that depend on RNA isolation and is amenable to high-throughput gene expression analysis of whole blood.

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