Comparison of a poly-
            l
            -lactide-co-
            ɛ
            -caprolactone and human amniotic membrane for urothelium tissue engineering applications

The reconstructive surgery of urothelial defects, such as severe hypospadias is susceptible to complications. The major problem is the lack of suitable grafting materials. Therefore, finding alternative treatments such as reconstruction of urethra using tissue engineering is essential. The aim of this study was to compare the effects of naturally derived acellular human amniotic membrane (hAM) to synthetic poly- l -lactide-co-ε-caprolactone (PLCL) on human urothelial cell (hUC) viability, proliferation and urothelial differentiation level. The viability of cells was evaluated using live/dead staining and the proliferation was studied using WST-1 measurement. Cytokeratin (CK)7/8 and CK19 were used to confirm that the hUCs maintained their phenotype on different biomaterials. On the PLCL, the cell number significantly increased during the culturing period, in contrast to the hAM, where hUC proliferation was the weakest at 7 and 14 days. In addition, the majority of cells were viable and maintained their phenotype when cultured on PLCL and cell culture plastic, whereas on the hAM, the viability of hUCs decreased with time and the cells did not maintain their phenotype. The PLCL membranes supported the hUC proliferation significantly more than the hAM. These results revealed the significant potential of PLCL membranes in urothelial tissue engineering applications.

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Supportive properties of basement membrane layer of human amniotic membrane enable development of tissue engineering applications

Cell and Tissue Banking ◽

10.1007/s10561-017-9680-z ◽

2018 ◽

Vol 19 (3) ◽

pp. 357-371 ◽

Cited By ~ 7

Author(s):

Sonia Iranpour ◽

Nasser Mahdavi-Shahri ◽

Raheleh Miri ◽

Halimeh Hasanzadeh ◽

Hamid Reza Bidkhori ◽

...

Keyword(s):

Tissue Engineering ◽

Basement Membrane ◽

Amniotic Membrane ◽

Human Amniotic Membrane ◽

Engineering Applications ◽

Membrane Layer

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Mineralized Human Amniotic Membrane as a Biomimetic Scaffold for Hard Tissue Engineering Applications

ACS Biomaterials Science & Engineering ◽

10.1021/acsbiomaterials.0c00881 ◽

2020 ◽

Vol 6 (11) ◽

pp. 6285-6298

Author(s):

Leila Sabouri ◽

Ali Farzin ◽

Azadeh Kabiri ◽

Peiman Brouki Milan ◽

Mojtaba Farahbakhsh ◽

...

Keyword(s):

Tissue Engineering ◽

Amniotic Membrane ◽

Human Amniotic Membrane ◽

Hard Tissue ◽

Engineering Applications ◽

Biomimetic Scaffold ◽

Hard Tissue Engineering

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The Fabrication of Human Amniotic Membrane Based Hydrogel for Cartilage Tissue Engineering Applications: A Preliminary Study

IFMBE Proceedings - 5th Kuala Lumpur International Conference on Biomedical Engineering 2011 ◽

10.1007/978-3-642-21729-6_205 ◽

2011 ◽

pp. 841-844 ◽

Cited By ~ 3

Author(s):

I. H. Hussin ◽

B. Pingguan-Murphy ◽

S. Z. Osman

Keyword(s):

Tissue Engineering ◽

Amniotic Membrane ◽

Cartilage Tissue Engineering ◽

Cartilage Tissue ◽

Human Amniotic Membrane ◽

Engineering Applications ◽

Preliminary Study

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Self-Healable Porous Polyampholyte Hydrogels with Higher Water Content as Cell Culture Scaffolds for Tissue Engineering Applications

ACS Applied Bio Materials ◽

10.1021/acsabm.0c00757 ◽

2020 ◽

Vol 3 (8) ◽

pp. 5446-5453 ◽

Cited By ~ 1

Author(s):

Jui-Hsiang Liu ◽

Yi-Hua Hung ◽

Kai-Ti Chang ◽

Chun-Yu Kao ◽

Yu-Ting Lin ◽

...

Keyword(s):

Tissue Engineering ◽

Cell Culture ◽

Water Content ◽

Engineering Applications

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Platelet-Rich Plasma in Tissue Engineering: Hype and Hope

European Surgical Research ◽

10.1159/000492415 ◽

2018 ◽

Vol 59 (3-4) ◽

pp. 265-275 ◽

Cited By ~ 15

Author(s):

Siegmund Lang ◽

Markus Loibl ◽

Marietta Herrmann

Keyword(s):

Tissue Engineering ◽

Cell Culture ◽

Growth Factors ◽

Progenitor Cells ◽

Platelet Rich Plasma ◽

Engineering Applications ◽

Preparation Methods ◽

Platelet Suspension ◽

A Cell ◽

Promising Source

Background: Platelet-rich plasma (PRP) refers to an enriched platelet suspension in plasma. In addition to the clinical application of PRP in the context of various orthopedic diseases and beyond, PRP and platelet lysate (PL) have been in focus in the field of tissue engineering. In this review, we discuss the application of PRP as a cell culture supplement and as part of tissue engineering strategies, particularly emphasizing current hurdles and ambiguities regarding the efficacy of PRP in these approaches. Summary: As a putative autologous replacement for animal-derived supplements such as fetal calf serum (FCS), PRP has been applied as cell culture supplement for the expansion of stem and progenitor cells for tissue engineering applications and cell therapies. Attributed to the high content of growth factors in platelets, PRP has been shown to promote cell growth, which was mostly superior to standard cultures supplemented with FCS, while the differentiation capacity of progenitor cells seems not to be affected. However, it was also suggested that cultivation of cells with PRP significantly alters the protein expression profile in cells in comparison to FCS, indicating that the influence of PRP on cell behavior should be thoroughly investigated. Moreover, different PRP preparation methods and donor variations have to be considered for the use of PRP under good manufacturing practice conditions. PRP has been used for various tissue engineering applications in the context of bone, cartilage, skin, and soft tissue repair, where most studies were conducted in the field of bone tissue engineering. These approaches take either advantage of the release of chemoattractive, angiogenic, proliferative, and putatively pro-regenerative growth factors from PRP, and/or the hydrogel properties of activated PRP, making it suitable as a cell delivery vehicle. In many of these studies, PRP is combined with biomaterials, cells, and in some cases recombinant growth factors. Although the experimental design often does not allow conclusions on the pro-regenerative effect of PRP itself, most publications report beneficial effects if PRP is added to the tissue-engineered construct. Furthermore, it was demonstrated that the release of growth factors from PRP may be tailored and controlled when PRP is combined with materials able to capture growth factors. Key Messages: Platelet-derived preparations such as PRP and PL represent a promising source of autologous growth factors, which may be applied as cell culture supplement or to promote regeneration in tissue-engineered constructs. Furthermore, activated PRP is a promising candidate as an autologous cell carrier. However, the studies investigating PRP in these contexts often show conflicting results, which most likely can be attributed to the lack of standardized preparation methods, particularly with regard to the platelet content and donor variation of PRP. Ultimately, the use of PRP has to be tailored for the individual application.

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