Human Amniotic Membrane: A Versatile Scaffold for Tissue Engineering

2018 ◽  
Vol 4 (7) ◽  
pp. 2226-2236 ◽  
Author(s):  
Julien H. Arrizabalaga ◽  
Matthias U. Nollert
Keyword(s):  
Tissue Engineering ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

Supportive properties of basement membrane layer of human amniotic membrane enable development of tissue engineering applications

2018 ◽  
Vol 19 (3) ◽  
pp. 357-371 ◽  
Author(s):  
Sonia Iranpour ◽  
Nasser Mahdavi-Shahri ◽  
Raheleh Miri ◽  
Halimeh Hasanzadeh ◽  
Hamid Reza Bidkhori ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Basement Membrane ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane ◽  
Engineering Applications ◽  
Membrane Layer

Tissue engineering for neurodegenerative diseases using human amniotic membrane and umbilical cord

2016 ◽  
Vol 18 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Anahí Sanluis-Verdes ◽  
Namibia Sanluis-Verdes ◽  
María Jesús Manso-Revilla ◽  
Antonio Manuel Castro-Castro ◽  
Jorge Pombo-Otero ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neurodegenerative Diseases ◽  
Umbilical Cord ◽  
Amniotic Membrane ◽  
Human Amniotic Membrane

scholarly journals Adipose-Derived Stromal Cells and Mineralized Extracellular Matrix Delivery by a Human Decellularized Amniotic Membrane in Periodontal Tissue Engineering

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 606
Author(s):  
Dilcele Silva Moreira Dziedzic ◽  
Bassam Felipe Mogharbel ◽  
Ana Carolina Irioda ◽  
Priscila Elias Ferreira Stricker ◽  
Maiara Carolina Perussolo ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Bone Tissue ◽  
Amniotic Membrane ◽  
Stromal Cells ◽  
Cell Attachment ◽  
Periodontal Regeneration ◽  
Human Amniotic Membrane ◽  
Micro Computed Tomography ◽  
Adipose Derived Stromal Cells

Periodontitis is a prevalent disease characterized by the loss of periodontal supporting tissues, bone, periodontal ligament, and cementum. The application of a bone tissue engineering strategy with Decellularized Human Amniotic Membrane (DAM) with adipose-derived stromal cells (ASCs) has shown to be convenient and valuable. This study aims to investigate the treatments of a rat periodontal furcation defect model with DAM, ASCs, and a mineralized extracellular matrix (ECM). Rat ASCs were expanded, cultivated on DAM, and with a bone differentiation medium for four weeks, deposited ECM on DAM. Periodontal healing for four weeks was evaluated by micro-computed tomography and histological analysis after treatments with DAM, ASCs, and ECM and compared to untreated defects on five consecutive horizontal levels, from gingival to apical. The results demonstrate that DAM preserves its structure during cultivation and healing periods, supporting cell attachment, permeation, bone deposition on DAM, and periodontal regeneration. DAM and DAM+ASCs enhance bone healing compared to the control on the gingival level. In conclusion, DAM with ASC or without cells and the ECM ensures bone tissue healing. The membrane supported neovascularization and promoted osteoconduction.

scholarly journals Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?

2021 ◽  
Vol 22 (11) ◽  
pp. 5976
Author(s):  
Sonia López ◽  
Lía Hoz ◽  
Eda Patricia Tenorio ◽  
Beatriz Buentello ◽  
Fátima Sofía Magaña ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Oral Mucosa ◽  
Amniotic Membrane ◽  
Embryonic Stem ◽  
Human Amniotic Membrane ◽  
Anatomical Location ◽  
Multilineage Differentiation ◽  
Therapeutic Alternative ◽  
E Cadherin

Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage differentiation capacity and express embryonic stem cells markers such as—Sox2, Oct3/4 and Nanog. We have used SHEM (supplemented hormonal epithelial medium) media and cultured hOMSCs over human amniotic membrane and determined the cell’s capacity to differentiate to an epithelial-like phenotype and to express corneal specific epithelial markers—CK3, CK12, CK19, Pan-cadherin and E-cadherin. Our results showed that hOMSCs possess the capacity to attach to the amniotic membrane and express CK3, CK19, Pan-Cadherin and E-Cadherin without induction with SHEM media and expressed CK12 or changed the expression pattern of E-Cadherin to a punctual-like feature when treated with SHEM media. The results observed in this study show that hOMSCs possess the potential to differentiate toward epithelial cells. In conclusion, our results revealed that hOMSCs readily express markers for corneal determination and could provide the ophthalmology field with a therapeutic alternative for tissue engineering to achieve corneal replacement when compared with other techniques. Nevertheless, further studies are needed to develop a predictable therapeutic alternative for cornea replacement.

scholarly journals Comparison of a poly- l -lactide-co- ɛ -caprolactone and human amniotic membrane for urothelium tissue engineering applications

2010 ◽  
Vol 8 (58) ◽  
pp. 671-677 ◽  
Author(s):  
Reetta Sartoneva ◽  
Suvi Haimi ◽  
Susanna Miettinen ◽  
Bettina Mannerström ◽  
Anne-Marie Haaparanta ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cell Culture ◽  
Amniotic Membrane ◽  
Reconstructive Surgery ◽  
Cell Number ◽  
Human Amniotic Membrane ◽  
Engineering Applications ◽  
Significant Potential ◽  
Human Urothelial Cell ◽  
Grafting Materials

The reconstructive surgery of urothelial defects, such as severe hypospadias is susceptible to complications. The major problem is the lack of suitable grafting materials. Therefore, finding alternative treatments such as reconstruction of urethra using tissue engineering is essential. The aim of this study was to compare the effects of naturally derived acellular human amniotic membrane (hAM) to synthetic poly- l -lactide-co-ε-caprolactone (PLCL) on human urothelial cell (hUC) viability, proliferation and urothelial differentiation level. The viability of cells was evaluated using live/dead staining and the proliferation was studied using WST-1 measurement. Cytokeratin (CK)7/8 and CK19 were used to confirm that the hUCs maintained their phenotype on different biomaterials. On the PLCL, the cell number significantly increased during the culturing period, in contrast to the hAM, where hUC proliferation was the weakest at 7 and 14 days. In addition, the majority of cells were viable and maintained their phenotype when cultured on PLCL and cell culture plastic, whereas on the hAM, the viability of hUCs decreased with time and the cells did not maintain their phenotype. The PLCL membranes supported the hUC proliferation significantly more than the hAM. These results revealed the significant potential of PLCL membranes in urothelial tissue engineering applications.

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