scholarly journals Surface plasmon resonance imaging detection of silver nanoparticle-tagged immunoglobulin

2011 ◽  
Vol 8 (61) ◽  
pp. 1204-1211 ◽  
Author(s):  
Sharmistha Paul ◽  
Deepen Paul ◽  
George R. Fern ◽  
Asim K. Ray

The detection sensitivity of silver nanoparticle (AgNP)-tagged goat immunoglobulin G (gIgG) microarrays was investigated by studying surface plasmon resonance (SPR) images captured in the visible wavelength range with the help of a Kretchmann-configured optical coupling set-up. The functionalization of anti-gIgG molecules on the AgNP surface was studied using transmission electron microscopy, photon correlation measurements and UV–visible absorption spectroscopy. A value of 1.3 × 10 7 M −1 was obtained for the antibody–antigen binding constant by monitoring the binding events at a particular resonance wavelength. The detection limit of this SPR imaging instrument is 6.66 nM of gIgG achieved through signal enhancement by a factor of larger than 4 owing to nanoparticle tagging with the antibody.

2012 ◽  
Vol 43 (10) ◽  
pp. 1385-1391 ◽  
Author(s):  
Yong-Hyok Kwon ◽  
Robert Ossig ◽  
Frank Hubenthal ◽  
Heinz-Detlef Kronfeldt

2010 ◽  
Vol 19 (11) ◽  
pp. 117310 ◽  
Author(s):  
Ji-Fei Wang ◽  
Hong-Jian Li ◽  
Zi-You Zhou ◽  
Xue-Yong Li ◽  
Ju Liu ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Wida Yanti ◽  
Asih Melati

<p><br />Halal foods and medicines are an absolute daily needs for the Muslim community in Indonesia. Therefore the authority institutions in indonesian goverment should ensure the availability of this. It is of course inseparable from the role of higher education through the development of its technology to develop halal detection of foods and drugs. This study is an effort to contribute to the Halal Research Center of UIN Sunan Kalijaga Yogyakarta through the biosensor development in halal detection foods and medicines based on biosensor SPR. This device using graphene materials to improve the detection sensitivity of pork gelatin material that is likely contained in foodstuffs and medicine. From analytical calculation and computation, enhancement of the SPR biosensor performance by involvement graphene it was shown through the ATR (Attenuated Total Reflectance) reflective curve. The result of this results was found the enhancement of the sensitivity 2,86 %.</p><p>Keyword: Surface Plasmon Resonance (SPR), Porcine Gelatin, Graphene, ATR</p>


2002 ◽  
Vol 738 ◽  
Author(s):  
Gang Xu ◽  
Masato Tazawa ◽  
Ping Jin

ABSTRACTA simple method was demonstrated to tune surface plasmon resonance wavelength of silver island films by introducing an underlayer. The tunability can be much enhanced by varying the underlayer medium. In combination with the size effects of metallic islands the plasmon wavelength can be readily adjusted throughout the visible spectral regions. The experimental results were interpreted based on a generalized Maxwell-Garnett theory.


Materials ◽  
2020 ◽  
Vol 13 (5) ◽  
pp. 1087
Author(s):  
Caroline R. Basso ◽  
Taís F. Cruz ◽  
Bruna L. Silva ◽  
Valber A. Pedrosa ◽  
João P. Araújo Junior

The aim of the current study is to introduce a methodology aimed at producing a biosensor that uses gold nanoparticles (AuNPs) to detect porcine circovirus 2 (PCV-2). This biosensor was based on AuNPs, which were modified with self-assembled monolayers (SAMs) and antibodies. The AuNPs’ surface and virus modification process applied to enable antibody binding was accompanied by localized surface plasmon resonance (LSPR), surface plasmon resonance (SPR), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDX). Virus quantification was possible by the light absorption difference in the spectrum at concentrations of 105, 106, 107, 108, and 109 DNA copies/mL PCV-2 in relation to quantitative PCR (qPCR), with an R2 value >0.98. The visualization of colorimetric changes in the different PCV-2 concentrations was possible without the use of equipment. The biosensor production methodology presented reproducibility and specificity, as well as easy synthesis and low cost. An enhanced version of it may be used in the future to replace traditional tests such as PCR.


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