scholarly journals Kajian Pengaruh Material Graphene pada kinerja Biosensor Berbasis Surface Plasmon Resonance (SPR) pada Deteksi Makanan Halal sebagai Pendukung Halal Research Center UIN Sunan Kalijaga Yogyakarta

2017 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Wida Yanti ◽  
Asih Melati
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Analytical Calculation ◽  
Detection Sensitivity ◽  
Research Center ◽  
Attenuated Total Reflectance ◽  
Muslim Community ◽  
Spr Biosensor

<p><br />Halal foods and medicines are an absolute daily needs for the Muslim community in Indonesia. Therefore the authority institutions in indonesian goverment should ensure the availability of this. It is of course inseparable from the role of higher education through the development of its technology to develop halal detection of foods and drugs. This study is an effort to contribute to the Halal Research Center of UIN Sunan Kalijaga Yogyakarta through the biosensor development in halal detection foods and medicines based on biosensor SPR. This device using graphene materials to improve the detection sensitivity of pork gelatin material that is likely contained in foodstuffs and medicine. From analytical calculation and computation, enhancement of the SPR biosensor performance by involvement graphene it was shown through the ATR (Attenuated Total Reflectance) reflective curve. The result of this results was found the enhancement of the sensitivity 2,86 %.</p><p>Keyword: Surface Plasmon Resonance (SPR), Porcine Gelatin, Graphene, ATR</p>

scholarly journals Recent advances in surface plasmon resonance imaging: detection speed, sensitivity, and portability

Nanophotonics ◽  
2017 ◽  
Vol 6 (5) ◽  
pp. 1017-1030 ◽  
Author(s):  
Youjun Zeng ◽  
Rui Hu ◽  
Lei Wang ◽  
Dayong Gu ◽  
Jianan He ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Imaging Techniques ◽  
Detection Sensitivity ◽  
Label Free ◽  
Spr Biosensor ◽  
Recent Advances ◽  
Wavelength Filter ◽  
Detection Speed

AbstractSurface plasmon resonance (SPR) biosensor is a powerful tool for studying the kinetics of biomolecular interactions because they offer unique real-time and label-free measurement capabilities with high detection sensitivity. In the past two decades, SPR technology has been successfully commercialized and its performance has continuously been improved with lots of engineering efforts. In this review, we describe the recent advances in SPR technologies. The developments of SPR technologies focusing on detection speed, sensitivity, and portability are discussed in details. The incorporation of imaging techniques into SPR sensing is emphasized. In addition, our SPR imaging biosensors based on the scanning of wavelength by a solid-state tunable wavelength filter are highlighted. Finally, significant advances of the vast developments in nanotechnology-associated SPR sensing for sensitivity enhancements are also reviewed. It is hoped that this review will provide some insights for researchers who are interested in SPR sensing, and help them develop SPR sensors with better sensitivity and higher throughput.

scholarly journals On the Use of Surface Plasmon Resonance Biosensing to Understand IgG-FcγR Interactions

2021 ◽  
Vol 22 (12) ◽  
pp. 6616
Author(s):  
Catherine Forest-Nault ◽  
Jimmy Gaudreault ◽  
Olivier Henry ◽  
Yves Durocher ◽  
Gregory De Crescenzo
Keyword(s):  
Surface Plasmon Resonance ◽  
Data Analysis ◽  
Surface Plasmon ◽  
Protein Interactions ◽  
Plasmon Resonance ◽  
Optical Biosensors ◽  
Label Free ◽  
Spr Biosensor ◽  
Therapeutic Monoclonal Antibodies

Surface plasmon resonance (SPR)-based optical biosensors offer real-time and label-free analysis of protein interactions, which has extensively contributed to the discovery and development of therapeutic monoclonal antibodies (mAbs). As the biopharmaceutical market for these biologics and their biosimilars is rapidly growing, the role of SPR biosensors in drug discovery and quality assessment is becoming increasingly prominent. One of the critical quality attributes of mAbs is the N-glycosylation of their Fc region. Other than providing stability to the antibody, the Fc N-glycosylation influences immunoglobulin G (IgG) interactions with the Fcγ receptors (FcγRs), modulating the immune response. Over the past two decades, several studies have relied on SPR-based assays to characterize the influence of N-glycosylation upon the IgG-FcγR interactions. While these studies have unveiled key information, many conclusions are still debated in the literature. These discrepancies can be, in part, attributed to the design of the reported SPR-based assays as well as the methodology applied to SPR data analysis. In fact, the SPR biosensor best practices have evolved over the years, and several biases have been pointed out in the development of experimental SPR protocols. In parallel, newly developed algorithms and data analysis methods now allow taking into consideration complex biomolecular kinetics. In this review, we detail the use of different SPR biosensing approaches for characterizing the IgG-FcγR interactions, highlighting their merit and inherent experimental complexity. Furthermore, we review the latest SPR-derived conclusions on the influence of the N-glycosylation upon the IgG-FcγR interactions and underline the differences and similarities across the literature. Finally, we explore new avenues taking advantage of novel computational analysis of SPR results as well as the latest strategies to control the glycoprofile of mAbs during production, which could lead to a better understanding and modelling of the IgG-FcγRs interactions.

Zinc oxide, gold and graphene-based surface plasmon resonance (SPR) biosensor for detection of pseudomonas like bacteria: A comparative study

Optik ◽  
2018 ◽  
Vol 172 ◽  
pp. 697-707 ◽  
Author(s):  
Angad S. Kushwaha ◽  
Anil Kumar ◽  
Rajeev Kumar ◽  
Monika Srivastava ◽  
S.K. Srivastava
Keyword(s):  
Zinc Oxide ◽  
Surface Plasmon Resonance ◽  
Comparative Study ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Spr Biosensor

scholarly journals Surface plasmon resonance biosensor for exosome detection based on reformative tyramine signal amplification activated by molecular aptamer beacon

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Wenqin Chen ◽  
Zhiyang Li ◽  
Wenqian Cheng ◽  
Tao Wu ◽  
Jia Li ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Lipid Membrane ◽  
Breast Cancer Patients ◽  
Her2 Positive ◽  
Chip Surface ◽  
Spr Biosensor ◽  
Aptamer Beacon

AbstractHuman epidermal growth factor receptor 2 (HER2)-positive exosomes play an extremely important role in the diagnosis and treatment options of breast cancers. Herein, based on the reformative tyramine signal amplification (TSA) enabled by molecular aptamer beacon (MAB) conversion, a label-free surface plasmon resonance (SPR) biosensor was proposed for highly sensitive and specific detection of HER2-positive exosomes. The exosomes were captured by the HER2 aptamer region of MAB immobilized on the chip surface, which enabled the exposure of the G-quadruplex DNA (G4 DNA) that could form peroxidase-like G4-hemin. In turn, the formed G4-hemin catalyzed the deposition of plentiful tyramine-coated gold nanoparticles (AuNPs-Ty) on the exosome membrane with the help of H2O2, generating a significantly enhanced SPR signal. In the reformative TSA system, the horseradish peroxidase (HRP) as a major component was replaced with nonenzymic G4-hemin, bypassing the defects of natural enzymes. Moreover, the dual-recognition of the surface proteins and lipid membrane of the desired exosomes endowed the sensing strategy with high specificity without the interruption of free proteins. As a result, this developed SPR biosensor exhibited a wide linear range from 1.0 × 104 to 1.0 × 107 particles/mL. Importantly, this strategy was able to accurately distinguish HER2-positive breast cancer patients from healthy individuals, exhibiting great potential clinical application. Graphical Abstract

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