scholarly journals Integration of binding peptide selection and multifunctional particles as tool-box for capture of soluble proteins in serum

2014 ◽  
Vol 11 (99) ◽  
pp. 20140718 ◽  
Author(s):  
Angela Maria Cusano ◽  
Filippo Causa ◽  
Raffaella Della Moglie ◽  
Nunzia Falco ◽  
Pasqualina Liana Scognamiglio ◽  
...  
Keyword(s):  
Protein Biomarkers ◽  
Necrosis Factor Alpha ◽  
Binding Peptide ◽  
Non Invasive ◽  
Phage Display Technique ◽  
Prognostic Tests ◽  
Human Tumour Necrosis Factor ◽  
Factor Alpha ◽  
Diagnostic Applications ◽  
Direct Use

In this paper, we report on a general approach for the detection of a specific tumoural biomarker directly in serum. Such detection is made possible using a protein-binding peptide selected through an improved phage display technique and then conjugated to engineered microparticles (MPs). Protein biomarkers represent an unlimited source of information for non-invasive diagnostic and prognostic tests; MP-based assays are becoming largely used in manipulation of soluble biomarkers, but their direct use in serum is hampered by the complex biomolecular environment. Our technique overcomes the current limitations as it produces a selective MP—engineered with an antifouling layer—that ‘captures’ the relevant protein staying impervious to the background. Our system succeeds in fishing-out the human tumour necrosis factor alpha directly in serum with a high selectivity degree. Our method could have great impact in soluble protein manipulation and detection for a wide variety of diagnostic applications.

scholarly journals IL-6 and TNF-α salivary levels according to the periodontal status in Portuguese pregnant women

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4710 ◽  
Author(s):  
Vanessa Machado ◽  
Maria Fernanda Mesquita ◽  
Maria Alexandra Bernardo ◽  
Ester Casal ◽  
Luís Proença ◽  
...  
Keyword(s):  
Periodontal Disease ◽  
Pregnant Women ◽  
Necrosis Factor Alpha ◽  
Periodontal Status ◽  
Non Invasive ◽  
Salivary Biomarkers ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Unstimulated Saliva ◽  
Factor Alpha

Background Periodontitis is associated with increased concentration of inflammatory markers and saliva has been proposed as a non-invasive diagnostic fluid in oral and systemic diseases. The levels of salivary biomarkers, such as cytokines, could potentially be used to distinguish periodontal healthy individuals from subjects with periodontal disease. The purpose of this study was to characterize the salivary levels of two inflammatory biomarkers associated with periodontitis, interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α), in order to assess whether these cytokines salivary levels could potentially be used to complement periodontitis pregnant women diagnose. Methods Forty-four pregnant women were distributed into three groups, according to their periodontal status: healthy, mild/moderate periodontitis and severe periodontitis. Unstimulated saliva was collected and analysis of TNF-α and IL-6 salivary levels were performed with Immulite®. Results Women with periodontitis exhibited significantly higher levels (p = 0.001) of salivary IL-6 and TNF-α compared with the healthy group: 25.1 (±11.2) pg/mL vs. 16.3 (±5.0) pg/mL and 29.7 (±17.2) pg/mL vs. 16.2 (±7.6) pg/mL, approximately 1.5 and 1.8 times more, respectively. Additionally, cytokines were significantly increased (p < 0.05) in severe periodontitis compared to periodontal healthy pregnant women. Conclusions These results revealed that IL-6 and TNF-α salivary biomarkers provide high discriminatory capacity for distinguishing periodontal disease from periodontal health in pregnant women.

scholarly journals Eosinophil-Derived Neurotoxin, Tumor Necrosis Factor Alpha, and Calprotectin as Non-Invasive Biomarkers of Food Protein-Induced Allergic Proctocolitis in Infants

2020 ◽  
Vol 9 (10) ◽  
pp. 3147
Author(s):  
Artur Rycyk ◽  
Beata Cudowska ◽  
Dariusz M. Lebensztejn
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis Factor Alpha ◽  
Tumor Necrosis ◽  
Control Group ◽  
Food Protein ◽  
Necrosis Factor Alpha ◽  
Non Invasive ◽  
Factor Alpha ◽  
Allergic Proctocolitis ◽  
Necrosis Factor

Diagnosis of non-IgE mediated food allergy presents a special challenge due to lack of a single, non-invasive diagnostic method. We selected three fecal biomarkers of allergic inflammation of gastrointestinal origin in order to improve the diagnostic process. Twenty-seven infants with symptoms of hematochezia were prospectively enrolled into this study. All patients underwent a complete differential diagnosis of rectal bleeding. Non-IgE mediated food allergy was confirmed by an open, oral food challenge. The control group included twenty-five infants with functional gastrointestinal disorders. Eosinophil-derived neurotoxin (EDN), tumor necrosis factor alpha (TNFα), and calprotectin concentration were measured in stools of all children by enzyme-linked immunosorbent assays (ELISA) using commercial kits. Median eosinophil-derived neurotoxin and calprotectin fecal levels were significantly higher in the study group than in the control group (p < 0.05). The difference of fecal tumor necrosis factor alpha concentration between both groups was not statistically significant (p > 0.05). The best diagnostic performance was reached in a combination of fecal calprotectin (fCal) and EDN i.e., 88.9% and 84%, respectively. Fecal EDN and fCAl are reliable tools in differentiating between food protein-induced allergic proctocolitis and gastrointestinal functional disorders in infants.

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