scholarly journals Ice sheet flow with thermally activated sliding. Part 2: the stability of subtemperate regions

2019 ◽  
Vol 475 (2231) ◽  
pp. 20190411
Author(s):  
E. Mantelli ◽  
C. Schoof
Keyword(s):  
Melting Point ◽  
Large Scale ◽  
Thermal Structure ◽  
Ice Sheet ◽  
Temperature Dependent ◽  
Length Scales ◽  
Thermally Activated ◽  
Potential Alternative ◽  
The Stability ◽  
Alternative Structure

The onset of sliding in ice sheets may not take the form of a sharp boundary between regions at the melting point, in which sliding is permitted, and regions below that temperature, in which there is no slip. Such a hard switch leads to the paradox of the bed naturally wanting to refreeze as soon as sliding has commenced. A potential alternative structure is a region of subtemperate sliding. Here temperatures are marginally below the melting point and sliding velocities slower than they would if the bed was fully temperate. Rather than being controlled by a standard sliding law, sliding velocities are then constrained by the need to maintain energy balance. This thermal structure arises in temperature-dependent sliding laws in the limit of strong sensitivity to temperature. Here, we analyse the stability of such subtemperate regions, showing that they are subject to a set of instabilities that occur at all length scales between ice thickness and ice sheet length. The fate of these instabilities is to cause the formation of patches of frozen bed, raising the possibility of highly complicated cold-to-temperate transitions with spatial structures at short length scales that cannot be resolved in large-scale ice sheet simulation codes.

scholarly journals Ice sheet flow with thermally activated sliding. Part 1: the role of advection

2019 ◽  
Vol 475 (2230) ◽  
pp. 20190410 ◽  
Author(s):  
E. Mantelli ◽  
M. Haseloff ◽  
C. Schoof
Keyword(s):  
Melting Point ◽  
Ice Sheet ◽  
Companion Paper ◽  
Ice Streams ◽  
Thermally Activated ◽  
Bed Temperature ◽  
Basal Sliding ◽  
Fast Motion ◽  
The Stability

Flow organization into systems of fast-moving ice streams is a well-known feature of ice sheets. Fast motion is frequently the result of sliding at the base of the ice sheet. Here, we consider how this basal sliding is first initiated as the result of changes in bed temperature. We show that an abrupt sliding onset at the melting point, with no sliding possible below that temperature, leads to rapid drawdown of cold ice and refreezing as the result of the increased temperature gradient within the ice, and demonstrate that this result holds regardless of the mechanical model used to describe the flow of ice. Using this as a motivation, we then consider the possibility of a region of ‘subtemperate sliding’ in which sliding at reduced velocities occurs in a narrow range of temperatures just below the melting point. We confirm that this prevents the rapid drawdown of ice and refreezing of the bed, and construct a simple numerical method for computing steady-state ice sheet profiles that include a subtemperate region. The stability of such an ice sheet is analysed in a companion paper.

scholarly journals Spatial variations in heat at the base of the Antarctic ice sheet from analysis of the thermal regime above subglacial lakes

1996 ◽  
Vol 42 (142) ◽  
pp. 501-509 ◽  
Author(s):  
Martin J. Siegert ◽  
Julian A. Dowdeswell
Keyword(s):  
Heat Flux ◽  
Melting Point ◽  
Thermal Model ◽  
Large Scale ◽  
Ice Sheet ◽  
Geothermal Heat ◽  
Vertical Advection ◽  
Basal Sliding ◽  
Pressure Melting ◽  
Subglacial Lakes

AbstractAntarctic subglacial lakes provide аn important boundary condition for thermal analysis of the ice sheet in that the basal ice temperature over lakes may be assumed to be at the pressure-melting point. We have used a one-dimensional vertical heat-transfer equation to determine theoretical temperature values for the ice-sheet base above 77 subglacial lakes identified from airborne radio-echo-sounding data covering 50% of Antarctica. Variations in our temperature results to below the pressure-melting temperature over lakes are due to either our estimate of the geothermal heat flux or a neglect of heat derived from (a) internal ice deformation and (b) basal sliding, in the thermal model. Our results indicate that, when the geothermal heat flux is set at 54 m W m−2, the ice-sheet base above 70% of the known Antarctic subglacial lakes is calculated to be at the pressure-melting value. These lakes are located mainly around Dome C, Ridge B and Vostok station. For the ice sheet above subglacial lakes located hundreds of kilometres from the ice divide, using the same thermal model, loss of heat due to vertical advection is calculated to be relatively high. In such regions, if the ice-sheet base is at the pressure-melting point, heat lost due to vertical advection must be supplemented by heat from other sources. For the three lakes beneath Terre Adélie and George V Land, for instance, the basal thermal gradient calculated to produce pressure melting at the ice-sheet base is equivalent to 1.5–2 times the value obtained when 54 m W m−2of geothermal heat is used as the sole basal thermal component. We suggest that, as distance from the ice divide increases, so too does the amount of heat due to internal ice deformation and basal sliding. Moreover, by considering the ice-sheet basal thermal characteristics above subglacial lakes which lie on the same ice flowline, we demonstrate empirically that the heat due to these horizontal ice-motion terms varies pseudo-exponentially with distance from the ice divide. The location along a flowline where a rapid increase in the basal heat gradient is calculated may correspond to the onset of large-scale basal sliding.

scholarly journals Spatial variations in heat at the base of the Antarctic ice sheet from analysis of the thermal regime above subglacial lakes

1996 ◽  
Vol 42 (142) ◽  
pp. 501-509 ◽  
Author(s):  
Martin J. Siegert ◽  
Julian A. Dowdeswell
Keyword(s):  
Heat Flux ◽  
Melting Point ◽  
Thermal Model ◽  
Large Scale ◽  
Ice Sheet ◽  
Geothermal Heat ◽  
Vertical Advection ◽  
Basal Sliding ◽  
Pressure Melting ◽  
Subglacial Lakes

AbstractAntarctic subglacial lakes provide аn important boundary condition for thermal analysis of the ice sheet in that the basal ice temperature over lakes may be assumed to be at the pressure-melting point. We have used a one-dimensional vertical heat-transfer equation to determine theoretical temperature values for the ice-sheet base above 77 subglacial lakes identified from airborne radio-echo-sounding data covering 50% of Antarctica. Variations in our temperature results to below the pressure-melting temperature over lakes are due to either our estimate of the geothermal heat flux or a neglect of heat derived from (a) internal ice deformation and (b) basal sliding, in the thermal model. Our results indicate that, when the geothermal heat flux is set at 54 m W m−2, the ice-sheet base above 70% of the known Antarctic subglacial lakes is calculated to be at the pressure-melting value. These lakes are located mainly around Dome C, Ridge B and Vostok station. For the ice sheet above subglacial lakes located hundreds of kilometres from the ice divide, using the same thermal model, loss of heat due to vertical advection is calculated to be relatively high. In such regions, if the ice-sheet base is at the pressure-melting point, heat lost due to vertical advection must be supplemented by heat from other sources. For the three lakes beneath Terre Adélie and George V Land, for instance, the basal thermal gradient calculated to produce pressure melting at the ice-sheet base is equivalent to 1.5–2 times the value obtained when 54 m W m−2 of geothermal heat is used as the sole basal thermal component. We suggest that, as distance from the ice divide increases, so too does the amount of heat due to internal ice deformation and basal sliding. Moreover, by considering the ice-sheet basal thermal characteristics above subglacial lakes which lie on the same ice flowline, we demonstrate empirically that the heat due to these horizontal ice-motion terms varies pseudo-exponentially with distance from the ice divide. The location along a flowline where a rapid increase in the basal heat gradient is calculated may correspond to the onset of large-scale basal sliding.

scholarly journals A three-dimensional ice-sheet flow solution

2009 ◽  
Vol 55 (191) ◽  
pp. 473-480 ◽  
Author(s):  
L.W. Morland
Keyword(s):  
Large Scale ◽  
Three Dimensional ◽  
Ice Sheet ◽  
Surface Profile ◽  
Temperature Dependent ◽  
Spatial Variables ◽  
Equation Problem ◽  
Dependent Rate ◽  
Horizontal Velocity Component ◽  
Spatial Coordinates

AbstractAn accurate three-dimensional reduced model (shallow-ice approximation) flow with velocity depending on all three spatial coordinates is constructed for the commonly adopted isotropic viscous law with temperature-dependent rate factor. The solution is for steady flow with a prescribed temperature distribution, but can be extended to flow with a coupled energy balance, and to unsteady flow. The accuracy hinges on the reduction to a two-point ordinary differential equation problem for the surface profile, on an unknown span, for which established accurate numerical methods are available. This is achieved by setting one horizontal velocity component in elliptic cylindrical coordinates to zero, but the other two components depend on all three spatial variables. While not of direct physical interest, such an ‘exact’ solution is valuable as a test solution for the large-scale numerical codes commonly used in ice-sheet modelling, which have not yet been subjected to such a comparison.

Construction and Functional Analysis of the Recombinant Bacteriocins Weissellicin-MBF from Weissella confusa MBF8-1

2020 ◽  
Vol 22 (1) ◽  
pp. 115-122
Author(s):  
Amarila Malik ◽  
Elita Yuliantie ◽  
Nisa Yulianti Suprahman ◽  
Theresa Linardi ◽  
Angelina Wening Widiyanti ◽  
...  
Keyword(s):  
Functional Analysis ◽  
Large Scale ◽  
Micrococcus Luteus ◽  
Affinity Column ◽  
Gene Expressions ◽  
His Tag ◽  
Recombinant Peptides ◽  
Weak Activity ◽  
Potential Alternative ◽  
Weissella Confusa

Background: Bacteriocins (Bac1, Bac2, and Bac3) from Weissella confusa MBF8-1, weissellicin- MBF, have been reported as potential alternative substances as well as complements to the existing antibiotics against many antimicrobial-resistant pathogens. Previously, the genes encoded in the large plasmid, pWcMBF8-1, and the spermicidal activity of their synthetic peptides, originally discovered Indonesia, have been studied. Three synthetic bacteriocins peptides of this weissellicin-MBF have been reported for their potential activities, i.e. antibacterial and spermicidal. Objective: The aim of this study was to construct the recombinant Bacteriocin (r-Bac) genes, as well as to investigate the gene expressions and their functional analysis. Method: Here, the recombinant Bacteriocin (r-Bac) genes were constructed and the recombinant peptides (r-Bac1, r-Bac2, and r-Bac3) in B. subtilis DB403 cells were produced on a large scale. After purification, using the His-tag affinity column, their potential bioactivities were measured as well as their antibacterial minimum inhibitory concentrations against Leuconostoc mesenteroides and Micrococcus luteus, were determined. Results: Pure His-tag-recombinant Bac1, Bac2, and Bac3 were obtained and they could inhibit the growth of L. mesenteroides and M. luteus. Conclusion: The recombinant bacteriocin could be obtained although with weak activity in inhibiting gram-positive bacterial growth.

scholarly journals Development and Characterization of Two Types of Surface Displayed Levansucrases for Levan Biosynthesis

Catalysts ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 757
Author(s):  
Huiyi Shang ◽  
Danni Yang ◽  
Dairong Qiao ◽  
Hui Xu ◽  
Yi Cao
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Molecular Weight ◽  
Large Scale ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Fusion Partner ◽  
Whole Cell ◽  
Food Industries ◽  
Yeast Surface ◽  
The Stability

Levan has wide applications in chemical, cosmetic, pharmaceutical and food industries. The free levansucrase is usually used in the biosynthesis of levan, but the poor reusability and low stability of free levansucrase have limited its large-scale use. To address this problem, the surface-displayed levansucrase in Saccharomyces cerevisiae were generated and evaluated in this study. The levansucrase from Zymomonas mobilis was displayed on the cell surface of Saccharomyces cerevisiae EBY100 using a various yeast surface display platform. The N-terminal fusion partner is based on a-agglutinin, and the C-terminal one is Flo1p. The yield of levan produced by these two whole-cell biocatalysts reaches 26 g/L and 34 g/L in 24 h, respectively. Meanwhile, the stability of the surface-displayed levansucrases is significantly enhanced. After six reuses, these two biocatalysts retained over 50% and 60% of their initial activities, respectively. Furthermore, the molecular weight and polydispersity test of the products suggested that the whole-cell biocatalyst of levansucrase displayed by Flo1p has more potentials in the production of levan with low molecular weight which is critical in certain applications. In conclusion, our method not only enable the possibility to reuse the enzyme, but also improves the stability of the enzyme.

scholarly journals INTRACISTRONIC MAPPING OF ELECTROPHORETIC SITES IN DROSOPHILA MELANOGASTER: FIDELITY OF INFORMATION TRANSFER BY GENE CONVERSION

Genetics ◽  
1974 ◽  
Vol 76 (2) ◽  
pp. 289-299
Author(s):  
Margaret McCarron ◽  
William Gelbart ◽  
Arthur Chovnick
Keyword(s):  
Drosophila Melanogaster ◽  
Information Transfer ◽  
Large Scale ◽  
Genetic Basis ◽  
Xanthine Dehydrogenase ◽  
Specific Protein ◽  
Wild Type ◽  
Electrophoretic Variation ◽  
The Stability ◽  
Recombination Experiments

ABSTRACT A convenient method is described for the intracistronic mapping of genetic sites responsible for electrophoretic variation of a specific protein in Drosophila melanogaster. A number of wild-type isoalleles of the rosy locus have been isolated which are associated with the production of electrophoretically distinguishable xanthine dehydrogenases. Large-scale recombination experiments were carried out involving null enzyme mutants induced on electrophoretically distinct wild-type isoalleles, the genetic basis for which is followed as a nonselective marker in the cross. Additionally, a large-scale recombination experiment was carried out involving null enzyme rosy mutants induced on the same wild-type isoallele. Examination of the electrophoretic character of crossover and convertant products recovered from the latter experiment revealed that all exhibited the same parental electrophoretic character. In addition to documenting the stability of the xanthine dehydrogenase electrophoretic character, this observation argues against a special mutagenesis hypothesis to explain conversions resulting from allele recombination studies.

scholarly journals The Dynamics of Subunit Rotation in a Eukaryotic Ribosome

Biophysica ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 204-221
Author(s):  
Frederico Campos Freitas ◽  
Gabriele Fuchs ◽  
Ronaldo Junio de Oliveira ◽  
Paul Charles Whitford
Keyword(s):  
Free Energy ◽  
Large Scale ◽  
Small Subunit ◽  
Free Energy Barrier ◽  
Temperature Dependent ◽  
Molecular Flexibility ◽  
Subunit Rotation ◽  
Biological Kinetics ◽  
Rate Limiting ◽  
Reversible Rotation

Protein synthesis by the ribosome is coordinated by an intricate series of large-scale conformational rearrangements. Structural studies can provide information about long-lived states, however biological kinetics are controlled by the intervening free-energy barriers. While there has been progress describing the energy landscapes of bacterial ribosomes, very little is known about the energetics of large-scale rearrangements in eukaryotic systems. To address this topic, we constructed an all-atom model with simplified energetics and performed simulations of subunit rotation in the yeast ribosome. In these simulations, the small subunit (SSU; ∼1 MDa) undergoes spontaneous and reversible rotation events (∼8∘). By enabling the simulation of this rearrangement under equilibrium conditions, these calculations provide initial insights into the molecular factors that control dynamics in eukaryotic ribosomes. Through this, we are able to identify specific inter-subunit interactions that have a pronounced influence on the rate-limiting free-energy barrier. We also show that, as a result of changes in molecular flexibility, the thermodynamic balance between the rotated and unrotated states is temperature-dependent. This effect may be interpreted in terms of differential molecular flexibility within the rotated and unrotated states. Together, these calculations provide a foundation, upon which the field may begin to dissect the energetics of these complex molecular machines.

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