The heterocysts of blue-green algae I. Ultrastructural integrity after isolation

1971 ◽  
Vol 178 (1051) ◽  
pp. 185-192 ◽  
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Internal Structure ◽  
Green Algae ◽  
Osmotic Shock ◽  
Anabaena Cylindrica ◽  
Vegetative Cells ◽  
Blue Green Algae ◽  
Mechanical Disruption ◽  
Physiological Properties

The heterocysts of Anabaena cylindrica were freed from filaments by differential disruption of vegetative cells using four techniques: mechanical disruption by French press, sonication, osmotic shock and lysozyme. The ultrastructure of isolated heterocysts was compared with that of heterocysts in intact filaments. The first three methods produced heterocysts whose internal structure showed different degrees of damage, involving in particular disruption of the heterocyst cell wall and plasmalemma. Isolation by the lysozyme method yielded heterocysts which appeared in the electron microscope to be intact and comparable with those of the untreated controls. These results suggest that earlier reports on the physiological properties of heterocysts isolated by means of the French press or sonication may require re-examination.

Freeze-Fracture Replication in the Ultrastructure of Blue-Green Algae

1967 ◽  
Vol 25 ◽  
pp. 74-75
Author(s):  
L. V. Leak
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Green Algae ◽  
Three Dimensional ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Photosynthetic Membranes ◽  
Blue Green Algae ◽  
Cell Biol ◽  
Cellular Components

Electron microscopic observations of freeze-fracture replicas of Anabaena cells obtained by the procedures described by Bullivant and Ames (J. Cell Biol., 1966) indicate that the frozen cells are fractured in many different planes. This fracturing or cleaving along various planes allows one to gain a three dimensional relation of the cellular components as a result of such a manipulation. When replicas that are obtained by the freeze-fracture method are observed in the electron microscope, cross fractures of the cell wall and membranes that comprise the photosynthetic lamellae are apparent as demonstrated in Figures 1 & 2.A large portion of the Anabaena cell is composed of undulating layers of cytoplasm that are bounded by unit membranes that comprise the photosynthetic membranes. The adjoining layers of cytoplasm are closely apposed to each other to form the photosynthetic lamellae. Occassionally the adjacent layers of cytoplasm are separated by an interspace that may vary in widths of up to several 100 mu to form intralamellar vesicles.

scholarly journals Studies on Nitrogen Fixation by Blue-Green Algae

1942 ◽  
Vol 19 (1) ◽  
pp. 78-87
Author(s):  
G. E. FOGG
Keyword(s):  
Nitrogen Fixation ◽  
Green Algae ◽  
Anabaena Cylindrica ◽  
Fixed Nitrogen ◽  
Blue Green Algae ◽  
Combined Nitrogen

1. Anabaena cylindrica Lemin. has been obtained in pure unialgal bacteria-free culture. 2. Due precautions having been taken against contamination by other organisms and error due to absorption of fixed nitrogen from the atmosphere, this alga has been shown to possess the capacity of fixing nitrogen. 3. Nitrogen fixation does not take place in the presence of a sufficient quantity of readily available combined nitrogen.

scholarly journals ​The Effect of Rhizobacteria in the Reducing drought Stress in Soybean (Glycine max L.)

2021 ◽  
Author(s):  
E. Oral ◽  
R. Tunçtürk ◽  
M. Tunçtürk
Keyword(s):  
Glycine Max ◽  
Green Algae ◽  
Dry Weight ◽  
Water Restriction ◽  
Blue Green Algae ◽  
Physiological Properties ◽  
Seedling Length ◽  
Glycine Max L ◽  
Wet Weight ◽  
Irrigation Levels

Background: This study was carried out to determine the effects of rhizobacteria and blue green algae applications on some physiological properties of soybean (Glycina max L.) grown under water stress. Methods: The experiment was conducted in factorial order with 4 replications according to the randomized plot trial design. In the research, Arýsoy variety belonging to soybean (Glycine max L.) species was used. In the experiment, it was aimed to examine the effects of three different irrigation levels (100, 50 and 25%) on some physiological characteristics of soybean with a control (control (B0), two different bacteria used. In this study, strains R1 of Azospirillum lipoferum bacteria, strains numbered 98 belonging to Bacillus megaterium bacteriaein and one blue green algae (Chlorella saccharophilia) were used. Result: According to the average data obtained, root length is 24.75 cm- 30.85 cm, seedling length 28.10-36.57 cm, root fresh weight 1.10-1.43 g, seedling wet weight 1.55-2.41 g, root dry weight 0.15-0.18 g, seedling dry weight 0.38-0.46 g, azote balance index 70.64-82.90 (dualex value), flavonol 0.375-0.398 (dualex value) and anthocyanin 0.016-0.045 (dualex value), with water restriction showed a decrease in most of these values. It has been determined that the rhizobacteria and blue-green algae have a decreasing and regulating effect on the physiological properties examined.

A study of several blue-green algae in the electron microscope

1962 ◽  
Vol 44 (3) ◽  
pp. 311-322 ◽  
Author(s):  
J. A. Chapman ◽  
M. R. J. Salton
Keyword(s):  
Electron Microscope ◽  
Green Algae ◽  
Blue Green Algae

Improved staining of extracellular polymer surrounding Eucapsis sp. and Anabena cylindrica: a comparative study

1974 ◽  
Vol 20 (5) ◽  
pp. 735-738
Author(s):  
Gerald D. Cagle
Keyword(s):  
Electron Microscope ◽  
Comparative Study ◽  
Green Algae ◽  
Freeze Drying ◽  
Ruthenium Red ◽  
Blue Green Algae ◽  
Modified Method ◽  
Ruthenium Red Staining ◽  
Extracellular Polymer

Extracellular polymer surrounding two blue-green algae, Eucapsis sp. (No. 1519) and Anabena cylindrica Lemm. (No. 629), was examined with the electron microscope. Conventional glutaraldehyde–OsO4 fixation, freeze-drying before fixation, and two ruthenium red staining procedures (Luft's method and the modified method of Cagle et al.) were used. The data obtained indicate that observation of extracellular polymer is successively enhanced over conventional fixation when (i) freeze-drying, (ii) Luft's ruthenium red method, and (iii) the modified method of Cagle et al. are used. Each of the methods was also observed to improve cytological detail, particularly in A. cylindrica.

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