Two new yeasts, Trichosporon debeurmannianum sp. nov. and Trichosporon dermatis sp. nov., transferred from the Cryptococcus humicola complex.

ABSTRACT Vanrija humicola ( Cryptococcus humicola ) strain UJ1 is a basidiomycetous yeast that produces d -aspartate oxidase, which is highly specific to d -aspartate. Here, we report the 22.6-Mb draft genome sequence of V. humicola strain UJ1, which comprises 22.6 Mb in 46 scaffolds, with an overall G+C content of 62.82%, comprising 46 scaffolds with an N 50 of 1.34 Mb.

Download Full-text

Use of DNA Sequencing Analysis To Confirm Fungemia Due to Trichosporon dermatis in a Pediatric Patient

Journal of Clinical Microbiology ◽

10.1128/jcm.44.3.1175-1177.2006 ◽

2006 ◽

Vol 44 (3) ◽

pp. 1175-1177 ◽

Cited By ~ 14

Author(s):

S. R. Gunn ◽

X. T. Reveles ◽

J. D. Hamlington ◽

L. C. Sadkowski ◽

T. L. Johnson-Pais ◽

...

Keyword(s):

Dna Sequencing ◽

Pediatric Patient ◽

Sequencing Analysis ◽

Trichosporon Dermatis ◽

Dna Sequencing Analysis

Download Full-text

The mycocidal, membrane-active complex of Cryptococcus humicola is a new type of cellobiose lipid with detergent features

Biochimica et Biophysica Acta (BBA) - Biomembranes ◽

10.1016/s0005-2736(01)00428-x ◽

2002 ◽

Vol 1558 (2) ◽

pp. 161-170 ◽

Cited By ~ 38

Author(s):

Evgeny O Puchkov ◽

Ulrich Zähringer ◽

Buko Lindner ◽

Tatiana V Kulakovskaya ◽

Ulrich Seydel ◽

...

Keyword(s):

Active Complex ◽

Cryptococcus Humicola ◽

New Type ◽

Cellobiose Lipid

Download Full-text

Regulation of d-Aspartate Oxidase Gene Expression by Pyruvate Metabolism in the Yeast Cryptococcus humicola

Microorganisms ◽

10.3390/microorganisms9122444 ◽

2021 ◽

Vol 9 (12) ◽

pp. 2444

Author(s):

Daiki Imanishi ◽

Sota Zaitsu ◽

Shouji Takahashi

Keyword(s):

Gene Expression ◽

Growth Retardation ◽

Wild Type Strain ◽

Transcriptional Level ◽

Oxidative Deamination ◽

Pyruvate Metabolism ◽

Oxidase Gene ◽

Cryptococcus Humicola ◽

In The Wild

d-Aspartate oxidase (DDO) is a peroxisomal flavoenzyme that catalyzes the oxidative deamination of acidic d-amino acids. In the yeast Cryptococcus humicola strain UJ1, the enzyme ChDDO is essential for d-Asp utilization and is expressed only in the presence of d-Asp. Pyruvate carboxylase (Pyc) catalyzes the conversion of pyruvate to oxaloacetate and is involved in the import and activation of certain peroxisomal flavoenzymes in yeasts. In this study, we analyzed the role of Pyc in the expression of ChDDO gene in C. humicola strain UJ1. PYC gene disruption (∆Chpyc1) in strain UJ1 resulted in growth retardation on glucose and NH4Cl medium. The growth was restored by supplying oxaloacetate from l-Asp or α-ketoglutarate by a transaminase. On the other hand, the supply of oxaloacetate from d-Asp by ChDDO was not able to prevent growth retardation because of a significant decrease in ChDDO gene expression at the transcriptional level. The addition of pyruvate significantly decreased ChDDO gene transcription in the ∆Chpyc1 strain but increased the same in the wild-type strain, even though the intracellular pyruvate content was similar in both strains. These results suggest that ChDDO gene expression might be regulated by pyruvate metabolism, as well as by the presence of d-Asp.

Download Full-text