scholarly journals A bovine macrophage cell line supports bovine herpesvirus-4 persistent infection

2001 ◽  
Vol 82 (5) ◽  
pp. 1181-1185 ◽  
Author(s):  
Gaetano Donofrio ◽  
Vicky L. van Santen
Keyword(s):  
Viral Genome ◽  
Bovine Herpesvirus ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Persistently Infected ◽  
Bovine Herpesvirus 4 ◽  
Bovine Macrophage ◽  
Herpesvirus 4

Although bovine herpesvirus-4 (BHV-4), a gammaherpesvirus lacking a clear disease association, has been demonstrated in many tissues during persistent BHV-4 infection, a likely site of virus persistence is in cells of the monocyte/macrophage lineage. To establish an in vitro model of persistent infection potentially useful for examining the molecular mechanisms of BHV-4 persistence/latency, we infected the bovine macrophage cell line BOMAC. Following extensive cell death, surviving cells were found to be persistently infected, maintaining the viral genome over many passages and producing low levels of infectious virus. Although selection was unnecessary for the maintenance of the viral genome, cells persistently infected with recombinant BHV-4 containing a neomycin-resistance gene could be selected with geneticin, thus confirming that persistent BHV-4 infection was compatible with cell survival and replication. Furthermore, persistent BHV-4 infection caused no decrease in the growth rate of BOMAC cells. Sodium butyrate, which reactivates latent gammaherpesviruses in vitro, or dexamethasone, which reactivates latent BHV-4 in vivo, increased viral DNA by 10- to 15-fold in persistently infected BOMAC cells. This suggests that reactivation of latent BHV-4 by dexamethasone in vivo might involve direct action of dexamethasone on latently infected cells.

Bovine herpesvirus 4 based vector interaction with liver cells in vitro and in vivo

2006 ◽  
Vol 136 (1-2) ◽  
pp. 126-136 ◽  
Author(s):  
Gaetano Donofrio ◽  
Eugenio Martignani ◽  
Enzo Poli ◽  
Claudia Lange ◽  
Filippo Maria Martini ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Liver Cells ◽  
Vector Interaction ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Bovine herpesvirus 4 ORF73 is dispensable for virus growth in vitro, but is essential for virus persistence in vivo

2010 ◽  
Vol 91 (10) ◽  
pp. 2574-2584 ◽  
Author(s):  
M. Thirion ◽  
B. Machiels ◽  
F. Farnir ◽  
G. Donofrio ◽  
L. Gillet ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Virus Persistence ◽  
Virus Growth ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus

Microbiology ◽  
2000 ◽  
Vol 81 (7) ◽  
pp. 1807-1814 ◽  
Author(s):  
Gaetano Donofrio ◽  
Sandro Cavirani ◽  
Vicky L. van Santen
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Viral Genome ◽  
Infectious Virus ◽  
Viral Gene Expression ◽  
Bovine Herpesvirus ◽  
Viral Gene ◽  
Persistently Infected ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Bovine herpesvirus-4 (BHV-4), a gammaherpesvirus lacking a clear disease association, productively infects multiple cell lines of various species and causes cell death. A human rhabdomyosarcoma cell line, RD-4, infected with BHV-4 produced low levels of early and late viral RNAs and infectious virus, but exhibited no cytopathic effect. Using a recombinant BHV-4 containing a neomycin-resistance gene, we established RD-4-derived cell lines persistently infected with BHV-4. The viral genome in these cells was predominantly circular. Because of drug selection, every cell contained a viral genome. In addition, all cells stained with a BHV-4-specific antiserum. Therefore, these cell lines are not carrier cultures. These cells produced infectious virus at all passages tested. Even though cells were selected and maintained at a concentration of geneticin at least 2·5 times that necessary to kill uninfected RD-4 cells, selected cells contained only approximately one viral genome per diploid host cell genome. Persistently infected cells grew more slowly than uninfected cells, even in the absence of drug. The slower growth of these cells suggests that any growth advantage conferred by multiple copies of the neomycin-gene-carrying viral genome might be offset by the detrimental effects of viral gene expression. This situation contrasts with other gammaherpesviruses, which are able to growth-transform cells.

scholarly journals Bovine Herpesvirus 4 Induces Apoptosis of Human Carcinoma Cell Lines In vitro and In vivo

2005 ◽  
Vol 65 (20) ◽  
pp. 9463-9472 ◽  
Author(s):  
Laurent Gillet ◽  
Benjamin Dewals ◽  
Frédéric Farnir ◽  
Laurence de Leval ◽  
Alain Vanderplasschen
Keyword(s):  
Cell Lines ◽  
Carcinoma Cell ◽  
Bovine Herpesvirus ◽  
Human Carcinoma ◽  
Carcinoma Cell Lines ◽  
Bovine Herpesvirus 4 ◽  
Human Carcinoma Cell ◽  
Herpesvirus 4

scholarly journals Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function

Reproduction ◽  
2007 ◽  
Vol 134 (1) ◽  
pp. 183-197 ◽  
Author(s):  
Gaetano Donofrio ◽  
Shan Herath ◽  
Chiara Sartori ◽  
Sandro Cavirani ◽  
Cesidio Filippo Flammini ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Stromal Cells ◽  
Bovine Herpesvirus ◽  
Endocrine Function ◽  
Uterine Disease ◽  
Endometrial Stromal Cells ◽  
Persistently Infected ◽  
Endometrial Cells ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

Bovinepostpartumuterine disease, metritis, affects about 40% of animals and is widely considered to have a bacterial aetiology. Although the γ-herpesvirus bovine herpesvirus 4 (BoHV-4) has been isolated from several outbreaks of metritis or abortion, the role of viruses in endometrial pathology and the mechanisms of viral infection of uterine cells are often ignored. The objectives of the present study were to explore the interaction, tropism and outcomes of BoHV-4 challenge of endometrial stromal and epithelial cells. Endometrial stromal and epithelial cells were purified and infected with a recombinant BoHV-4 carrying an enhanced green fluorescent protein (EGFP) expression cassette to monitor the establishment of infection. BoHV-4 efficiently infected both stromal and epithelial cells, causing a strong non-apoptotic cytopathic effect, associated with robust viral replication. The crucial step for the BoHV-4 endometriotropism appeared to be after viral entry as there was enhanced transactivation of the BoHV-4 immediate early 2 gene promoter following transient transfection into the endometrial cells. Infection with BoHV-4 increased cyclooxygenase 2 protein expression and prostaglandin estradiol secretion in endometrial stromal cells, but not epithelial cells. Bovine macrophages are persistently infected with BoHV-4, and co-culture with endometrial stromal cells reactivated BoHV-4 replication in the persistently infected macrophages, suggesting a symbiotic relationship between the cells and virus. In conclusion, the present study provides evidence of cellular and molecular mechanisms, supporting the concept that BoHV-4 is a pathogen associated with uterine disease.

Identification of tyrosine 706 in the kinase insert as the major colony-stimulating factor 1 (CSF-1)-stimulated autophosphorylation site in the CSF-1 receptor in a murine macrophage cell line

1990 ◽  
Vol 10 (6) ◽  
pp. 2991-3002
Author(s):  
P van der Geer ◽  
T Hunter
Keyword(s):  
Cell Line ◽  
Murine Macrophage ◽  
Colony Stimulating Factor ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Major Site ◽  
Murine Macrophage Cell Line ◽  
Stimulating Factor

The receptor for colony-stimulating factor 1 (CSF-1) is a ligand-activated protein-tyrosine kinase. It has been shown previously that the CSF-1 receptor is phosphorylated on serine in vivo and that phosphorylation on tyrosine can be induced by stimulation with CSF-1. We studied the phosphorylation of the CSF-1 receptor by using the BAC1.2F5 murine macrophage cell line, which naturally expresses CSF-1 receptors. Two-dimensional tryptic phosphopeptide mapping showed that the CSF-1 receptor is phosphorylated on several different serine residues in vivo. Stimulation with CSF-1 at 37 degrees C resulted in rapid phosphorylation on tyrosine at one major site and one or two minor sites. We identified the major site as Tyr-706. The identity of Tyr-706 was confirmed by mutagenesis. This residue is located within the kinase insert domain. There was no evidence that Tyr-973 (equivalent to Tyr-969 in the human CSF-1 receptor) was phosphorylated following CSF-1 stimulation. When cells were stimulated with CSF-1 at 4 degrees C, additional phosphotyrosine-containing phosphopeptides were detected and the level of phosphorylation of the individual phosphotyrosine-containing phosphopeptides was substantially increased. In addition, we show that CSF-1 receptors are capable of autophosphorylation at six to eight major sites in vitro.

Comparison of the In Vivo and In Vitro Proliferation of Monoblasts, Promonocytes, and the Macrophage Cell Line J774

1982 ◽  
pp. 175-187 ◽  
Author(s):  
R. van Furth ◽  
Th. J. L. M. Goud ◽  
J. W. M. van der Meer ◽  
A. Blussé van Oud Alblas ◽  
M. M. C. Diesselhoff-den Dulk ◽  
...  
Keyword(s):  
Cell Line ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
In Vitro Proliferation

scholarly journals Molecular Characterization of the First Bovine Herpesvirus 4 (BoHV-4) Strains Isolated from In Vitro Bovine Embryos production in Argentina

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0132212 ◽  
Author(s):  
Erika González Altamiranda ◽  
Julieta M. Manrique ◽  
Sandra E. Pérez ◽  
Glenda L. Ríos ◽  
Anselmo C. Odeón ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Bovine Herpesvirus ◽  
Bovine Embryos ◽  
Bovine Herpesvirus 4 ◽  
Herpesvirus 4

scholarly journals Antibodies Induced by Lipoarabinomannan in Bovines: Characterization and Effects on the Interaction betweenMycobacterium aviumSubsp.paratuberculosisand MacrophagesIn Vitro

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Ana Jolly ◽  
Silvia Beatriz Colavecchia ◽  
Bárbara Fernández ◽  
Eloy Fernández ◽  
Silvia Leonor Mundo
Keyword(s):  
Immune Response ◽  
Mycobacterium Avium ◽  
Active Role ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Specific Antibodies ◽  
Humoral Immune ◽  
Bovine Macrophage

Lipoarabinomannan (LAM) is a major glycolipidic antigen on the mycobacterial envelope. The aim of this study was to characterize the humoral immune response induced by immunization with a LAM extract in bovines and to evaluate the role of the generated antibodies in thein vitroinfection of macrophages withMycobacterium aviumsubsp.paratuberculosis(MAP). Sera from fourteen calves immunized with LAM extract or PBS emulsified in Freund's Incomplete Adjuvant and from five paratuberculosis-infected bovines were studied. LAM-immunized calves developed specific antibodies with IgG1 as the predominant isotype. Serum immunoglobulins were isolated and their effect was examined in MAP ingestion and viability assays using a bovine macrophage cell line. Our results show that the antibodies generated by LAM immunization significantly increase MAP ingestion and reduce its intracellular viability, suggesting an active role in this model.

scholarly journals p38/TF/HIF-α Signaling Pathway Participates in the Progression of CIPN in Mice

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Yang Yang ◽  
Liang Hu ◽  
Chaoyu Wang ◽  
Xing Yang ◽  
Ling Song ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Coagulation Factor ◽  
Gelatin Zymography ◽  
Pathological Process ◽  
Macrophage Cell Line ◽  
Macrophage Cell ◽  
Microcirculatory Disturbance ◽  
Molecular Signal

Chemotherapy induced peripheral neuropathy (CIPN) is a serious adverse effect of chemotherapeutics with limited pathogenetic mechanism been known. Whether microcirculatory disturbance is involved in CIPN has not been reported. Considering that tissue factor (TF) is an endogenous coagulation factor, we hypothesize CIPN may be induced by the high expression of TF in macrophages and sciatic nerve, which induces the molecular signal related to ischemia and hypoxia. Oxaliplatin (L-OHP) was used to establish CIPN model. Von Frey Hairs was used to measure nociception. The murine macrophage cell line Raw 264.7 was used for cell experiments. Gelatin zymography and western blotting were used to measure the activity or expression of protein. TF expression and MMP-9/2 activity in sciatic nerve and blood are significantly increased by L-OHP. L-OHP increased the release of HSP70 from macrophage and enhanced the expression of p-p38 and HIF-1α in vivo and in vitro. Hirudin significantly suppressed the overexpression of p38, HIF-1α and activation of MMP-9/2 induced by L-OHP and attenuated CIPN in mice. This study suggests that a novel HSP70-TLR-4-p38-TF-HIF-1a axis may play a pivotal role in the pathological process of CIPN. It is also shown that the use of anticoagulant Hirudin can inhibit the above mechanisms and improve CIPN.

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