scholarly journals Characterisation of Staphylococcus intermedius isolates from canine pyoderma and from healthy carriers by SDS-PAGE of exoproteins, immunoblotting and restriction endonuclease digest analysis

1993 ◽  
Vol 39 (6) ◽  
pp. 429-433 ◽  
Author(s):  
R. P. Allaker ◽  
N. Garrett ◽  
L. Kent ◽  
W. C. Noble ◽  
D. H. Lloyd
Keyword(s):  
Restriction Endonuclease ◽  
Staphylococcus Intermedius ◽  
Sds Page ◽  
Restriction Endonuclease Digest ◽  
Healthy Carriers

scholarly journals Restriction endonuclease characterization of resistant plasmids in Enterobacteriaceae isolated from children in the Sudan

1989 ◽  
Vol 103 (3) ◽  
pp. 487-496 ◽  
Author(s):  
P. Shears ◽  
G. Suliman ◽  
C. A. Hart
Keyword(s):  
Antimicrobial Resistance ◽  
Restriction Endonuclease ◽  
Restriction Enzymes ◽  
Surveillance Systems ◽  
High Prevalence ◽  
Resistance Patterns ◽  
Reproducible Method ◽  
Restriction Endonuclease Digest

SUMMARYThe investigation of plasmid similarity is an important component in the surveillance of antimicrobial resistance and in the detection of epidemic plasmids. The use of restriction endonucleases in the classification of transferable, multiply-resistant plasmids from faecal Enterobacteriaceae isolated at the Children's Emergency Hospital, Khartoum was investigated. Twenty-four transconjugant plasmids, coding for 11 different resistance patterns, each of molecular weight 62 MDa. were studied using four restriction enzymes;PstI,EcoR I,HindIII andAraII. Fifteen different digest profiles were obtained. Restriction profiles discriminated between plasmids with differing resistance patterns and demonstrated homology of plasmids with common resistance patterns. Restriction endonuclease digest patterns provide a potentially rapid and reproducible method of plasmid classification, that could contribute towards surveillance systems in tropical countries with a high prevalence of antimicrobial resistance.

Apparent Identity of Chromoplast and Chloroplast DNA in the Daffodil, Narcissus pseudonarcissus

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1101-1103 ◽  
Author(s):  
John A. Thompson
Keyword(s):  
Molecular Weight ◽  
Chloroplast Dna ◽  
Restriction Endonuclease ◽  
Agarose Gels ◽  
Narcissus Pseudonarcissus ◽  
Ecori Restriction ◽  
Restriction Endonuclease Digest ◽  
Chloroplast Dnas

Abstract A comparison of the EcoRI restriction endonuclease digest fragments from daffodil chromoplast and chloroplast DNAs on agarose gels has yielded identical results. The sum of the fragments for each DNA gives a minim um molecular weight of 98.6 × 106.

Comparison of mycoplasma ovipneumoniae isolates using bacterial restriction endonuclease DNA analysis and SDS-PAGE

1985 ◽  
Vol 10 (6) ◽  
pp. 541-548 ◽  
Author(s):  
Alison J. Mew ◽  
G. Ionas ◽  
J.K. Clarke ◽  
A.J. Robinson ◽  
R.B. Marshall
Keyword(s):  
Restriction Endonuclease ◽  
Dna Analysis ◽  
Sds Page ◽  
Mycoplasma Ovipneumoniae
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