scholarly journals Brassicibacter thermophilus sp. nov., a thermophilic bacterium isolated from coastal sediment

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 2870-2874 ◽  
Author(s):  
Bing Wang ◽  
Shi-Qi Ji ◽  
Xin-Xin Tian ◽  
Ling-Yun Qu ◽  
Fu-Li Li
Keyword(s):  
Lipid Analysis ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
Sea Salt ◽  
Rrna Gene ◽  
Optimal Growth Temperature ◽  
Coastal Marine ◽  
Coastal Marine Sediment ◽  
Bacterium Strain

A novel thermophilic, obligately anaerobic bacterium, strain Cel2fT, was isolated from a cellulolytic community enriched from coastal marine sediment. Cells were Gram-stain-negative, non-motile, non-spore-forming and rod-shaped. Optimal growth temperature and pH of strain Cel2fT were 55 °C and pH 7.0, respectively. NaCl was essential for the growth of strain Cel2fT and the strain showed enhanced growth in the presence of sea salt; the optimum sea salt concentration for growth was 7 % (w/v). Thiosulfate, sulfate and sulfite were potential electron acceptors. The major fatty acids of strain Cel2fT were iso-C15 : 0, C16 : 0, and C18 : 0. Polar lipid analysis indicated the presence of phosphatidylethanolamine and phosphatidylglycerol. Strain Cel2fT contained menaquinone MK-7 as the isoprenoid quinone, and the DNA G+C content was 31.3 mol%. Phylogenetic analysis revealed that the nearest relative of strain Cel2fT was Brassicibacter mesophilus BMT with 93.8 % 16S rRNA gene sequence similarity. Based on phenotypic, chemotaxonomic and phylogenetic characteristics, strain Cel2fT represents a novel species of genus Brassicibacter, for which the name Brassicibacter thermophilus sp. nov. is proposed. The type strain is Cel2fT ( = JCM 30480T = CGMCC 1.5200T).

scholarly journals Thermaerobacter litoralis sp. nov., a strictly aerobic and thermophilic bacterium isolated from a coastal hydrothermal field

2006 ◽  
Vol 56 (7) ◽  
pp. 1531-1534 ◽  
Author(s):  
Reiji Tanaka ◽  
Satoshi Kawaichi ◽  
Hiroshi Nishimura ◽  
Yoshihiko Sako
Keyword(s):  
Dna Hybridization ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Hydrothermal Field ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
The Novel ◽  
Optimum Ph ◽  
Kagoshima Prefecture ◽  
Bacterium Strain

A novel thermophilic bacterium, strain KW1T, was isolated from a coastal hydrothermal field on the Satsuma Peninsula, Kagoshima Prefecture, Japan. The variably Gram-stained cells were motile rods with flagella, did not form spores and proliferated at 52–78 °C (optimum, 70 °C), pH 5–8 (optimum, pH 7) and 0–4.5 % NaCl (optimum, 1.0 %). The novel isolate was a strictly aerobic heterotroph that utilized complex proteinaceous substrates as well as a variety of carboxylic acids and amino acids. The G+C content of the genomic DNA was 70.8 mol%. Analysis of 16S rRNA gene sequences indicated that strain KW1T is closely related to Thermaerobacter subterraneus C21T (98.4 % sequence similarity). However, the DNA–DNA hybridization value for strain KW1T and T. subterraneus ATCC BAA-137T was below 46 %. On the basis of the molecular and physiological traits of strain KW1T, it represents a novel species of the genus Thermaerobacter, for which the name Thermaerobacter litoralis sp. nov. is proposed. The type strain is KW1T (=JCM 13210T=DSM 17372T).

Brockia lithotrophica gen. nov., sp. nov., an anaerobic thermophilic bacterium from a terrestrial hot spring

2013 ◽  
Vol 63 (Pt_2) ◽  
pp. 479-483 ◽  
Author(s):  
Anna A. Perevalova ◽  
Ilya V. Kublanov ◽  
R. V. Baslerov ◽  
Gengxin Zhang ◽  
Elizaveta A. Bonch-Osmolovskaya
Keyword(s):  
Hot Spring ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Content Type ◽  
Positive Type ◽  
Link Type ◽  
Terrestrial Hot Spring

A novel thermophilic bacterium, strain Kam1851T, was isolated from a terrestrial hot spring of the Uzon Caldera, Kamchatka Peninsula, Russia. Cells of strain Kam1851T were spore-forming rods with a Gram-positive type of cell wall. Growth was observed between 46 and 78 °C, and pH 5.5–8.5. The optimal growth (doubling time, 6.0 h) was at 60–65 °C and pH 6.5. The isolate was an obligate anaerobe growing in pre-reduced medium only. It grew on mineral medium with molecular hydrogen or formate as electron donors, and elemental sulfur, thiosulfate or polysulfide as electron acceptors. The main cellular fatty acids were C16 : 0 (34.2 %), iso-C16 : 0 (18 %), C18 : 0 (12.8 %) and iso-C17 : 0 (11.1 %). The G+C content of the genomic DNA of strain Kam1851T was 63 mol%. 16S rRNA gene sequence analysis showed that strain Kam1851T belonged to the order Thermoanaerobacterales , but it was not closely related to representatives of any genera with validly published names. The most closely related strains, which had no more than 89.2 % sequence similarity, were members of the genera Ammonifex and Caldanaerobacter . On the basis of its phylogenetic position and novel phenotypic features, isolate Kam1851T is proposed to represent a novel species in a new genus, Brockia lithotrophica gen. nov., sp. nov.; the type strain of Brockia lithotrophica is Kam1851T ( = DSM 22653T = VKM B-2685T).

scholarly journals Thermobacillus composti sp. nov., a moderately thermophilic bacterium isolated from a composting reactor

2007 ◽  
Vol 57 (7) ◽  
pp. 1473-1477 ◽  
Author(s):  
Keiko Watanabe ◽  
Norio Nagao ◽  
Shuich Yamamoto ◽  
Tatsuki Toda ◽  
Norio Kurosawa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Thermophilic Bacterium ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Moderately Thermophilic

A Gram-negative, rod-shaped, spore-forming and moderately thermophilic bacterium, strain KWC4T, was isolated from a composting reactor. Cells of strain KWC4T were 2.0–5.0 μm long and 0.5–0.7 μm in diameter. Strain KWC4T grew aerobically at 32–61 °C, with optimal growth occurring at 50 °C. It grew at pH 5.6–10.1, with optimal growth at around pH 9.0. The optimum NaCl concentration for growth was almost 0 % (w/v), but strain KWC4T was moderately halotolerant and was able to grow at NaCl concentrations up to 4.4 % (w/v). The DNA G+C content of strain KWC4T was 60.0 mol%. The major fatty acids were iso-16 : 0 (39.0 %) and anteiso-15 : 0 (33.3 %). Based on 16S rRNA gene sequence similarity data, strain KWC4T belonged to the genus Thermobacillus and was related to Thermobacillus xylanilyticus. However, strain KWC4T had a 38 bp insertion sequence located near the 3′ end of its 16S rRNA gene that was not present in T. xylanilyticus. The 16S rRNA gene sequence similarity value between strain KWC4T and T. xylanilyticus was 95.7 %. The DNA–DNA hybridization value between strain KWC4T and T. xylanilyticus strain XET was 66 %. On the basis of phenotypic and genotypic evidence, strain KWC4T (=DSM 18247T=JCM 13945T) is the type strain of a novel species, for which the name Thermobacillus composti sp. nov. is proposed.

scholarly journals Brevibacillus aydinogluensis sp. nov., a moderately thermophilic bacterium isolated from Karakoc hot spring

2012 ◽  
Vol 62 (Pt_4) ◽  
pp. 849-855 ◽  
Author(s):  
Kadriye Inan ◽  
Sabriye Canakci ◽  
Ali Osman Belduz ◽  
Fikrettin Sahin
Keyword(s):  
Type Species ◽  
Hot Spring ◽  
Lipid Analysis ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Content Type ◽  
Moderately Thermophilic ◽  
Link Type ◽  
Gene Sequence Analysis

Two Gram-positive, moderately thermophilic, endospore-forming, rod-shaped, motile bacteria, designated PDF25T and PDF30, were isolated from Karakoc hot spring in the province of Izmir, Turkey, and were characterized in order to determine their phylogenetic positions. 16S rRNA gene sequence analysis revealed that the two strains belonged to the genus Brevibacillus ; strain PDF25T showed highest sequence similarity to strain PDF30 (99.4 %) and Brevibacillus thermoruber DSM 7064T (98.5 %). The major fatty acids of strain PDF25T were iso-C15 : 0 (39.30 %), anteiso-C15 : 0 (26.10 %) and iso-C16 : 0 (14.75 %). Polar lipid analysis revealed diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, and a variety of unidentified aminophospholipids, phospholipids and aminolipids. The major isoprenoid quinone was MK-7. The G+C content of the genomic DNA was 56.09 mol%. DNA–DNA hybridization experiments revealed 58 % relatedness between strain PDF25T and B. thermoruber DSM 7064T. Based on these data, the two strains are considered to represent a novel species of the genus Brevibacillus , for which the name Brevibacillus aydinogluensis sp. nov. is proposed. The type strain is PDF25T ( = DSM 24395T = LMG 26289T).

scholarly journals Chthonomonas calidirosea gen. nov., sp. nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10

2011 ◽  
Vol 61 (10) ◽  
pp. 2482-2490 ◽  
Author(s):  
Kevin C.-Y. Lee ◽  
Peter F. Dunfield ◽  
Xochitl C. Morgan ◽  
Michelle A. Crowe ◽  
Karen M. Houghton ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Phylogenetic Position ◽  
Crystalline Cellulose ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
New Class ◽  
Candidate Division ◽  
Bacterium Strain ◽  
Heated Soil

An aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49T, was isolated from geothermally heated soil at Hell’s Gate, Tikitere, New Zealand. On the basis of 16S rRNA gene sequence similarity, T49T is the first representative of a new class in the newly described phylum Armatimonadetes, formerly known as candidate division OP10. Cells of strain T49T stained Gram-negative and were catalase-positive and oxidase-negative. Cells possessed a highly corrugated outer membrane. The major fatty acids were 16 : 0, i17 : 0 and ai17 : 0. The G+C content of the genomic DNA was 54.6 mol%. Strain T49T grew at 50–73 °C with an optimum temperature of 68 °C, and at pH 4.7–5.8 with an optimum growth pH of 5.3. A growth rate of 0.012 h−1 was observed under optimal temperature and pH conditions. The primary respiratory quinone was MK-8. Optimal growth was achieved in the absence of NaCl, although growth was observed at NaCl concentrations as high as 2 % (w/v). Strain T49T was able to utilize mono- and disaccharides such as cellobiose, lactose, mannose and glucose, as well as branched or amorphous polysaccharides such as starch, CM-cellulose, xylan and glycogen, but not highly linear polysaccharides such as crystalline cellulose or cotton. On the basis of its phylogenetic position and phenotypic characteristics, we propose that strain T49T represents a novel bacterial genus and species within the new class Chthonomonadetes classis nov. of the phylum Armatimonadetes. The type strain of Chthonomonas calidirosea gen. nov., sp. nov. is T49T ( = DSM 23976T = ICMP 18418T).

scholarly journals Flavobacterium glaciei sp. nov., a novel psychrophilic bacterium isolated from the China No.1 glacier

2006 ◽  
Vol 56 (12) ◽  
pp. 2921-2925 ◽  
Author(s):  
De-Chao Zhang ◽  
He-Xiang Wang ◽  
Hong-Can Liu ◽  
Xiu-Zhu Dong ◽  
Pei-Jin Zhou
Keyword(s):  
Phylogenetic Analysis ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Aerobic Bacterium ◽  
Psychrophilic Bacterium ◽  
Obligate Aerobic ◽  
Bacterium Strain ◽  
The Common ◽  
Sequence Similarities

A novel psychrophilic, yellow-pigmented and obligate aerobic bacterium, strain 0499T, was isolated from the China No.1 glacier. Strain 0499T displayed the common phenotypic and chemotaxonomic features of the genus Flavobacterium, containing menaquinone-6 (MK-6) as the major quinone and C15 : 0, iso-C15 : 0, C17 : 1 ω6c and summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH) as the major fatty acids. Optimal growth occurred at 21 °C. The genomic DNA G+C content was 36.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain 0499T was related to members of the genus Flavobacterium, sharing the highest sequence similarities with Flavobacterium succinicans (97.9 %), Flavobacterium granuli (97.4 %) and Flavobacterium hydatis (97.2 %). On the basis of phenotypic characteristics, phylogenetic analysis and DNA–DNA relatedness data, a novel species Flavobacterium glaciei is proposed with strain 0499T (=CGMCC 1.5380T=JCM 13953T) as the type strain.

scholarly journals Luteimonas composti sp. nov., a moderately thermophilic bacterium isolated from food waste

2007 ◽  
Vol 57 (4) ◽  
pp. 741-744 ◽  
Author(s):  
Chiu-Chung Young ◽  
Peter Kämpfer ◽  
Wen-Ming Chen ◽  
Wen-Shao Yen ◽  
A. B. Arun ◽  
...  
Keyword(s):  
Food Waste ◽  
Type Strain ◽  
Sequence Similarity ◽  
Thermophilic Bacterium ◽  
Physiological Data ◽  
Rrna Gene ◽  
Phenotypic Properties ◽  
Moderately Thermophilic ◽  
Gene Sequence Analysis ◽  
Bacterium Strain

A yellow-pigmented, Gram-negative, rod-shaped bacterium (strain CC-YY255T) was isolated from compost generated from food waste collected from Kinmen County, Taiwan. 16S rRNA gene sequence analysis indicated that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Luteimonas; its closest neighbour was the type strain of Luteimonas mephitis (94.4 % sequence similarity). The isolate was distinguished from Luteimonas mephitis on the basis of several phenotypic properties. The organism utilized glucose, maltose, gentiobiose, melibiose and turanose and only a few organic acids (acetate, propionate) and amino acids (l-alanyl glycine, glycyl l-aspartic acid and glycyl l-glutamic acid) as substrates. The fatty acid profile was slightly different from that reported for Luteimonas mephitis. It is evident from the genotypic, chemotaxonomic and physiological data presented that strain CC-YY255T represents a novel species of the genus Luteimonas, for which the name Luteimonas composti sp. nov. is proposed. The type strain is CC-YY255T (=CCUG 53595T=CIP 109311T=BCRC 17598T).

scholarly journals Belliella kenyensis sp. nov., isolated from an alkaline lake

2015 ◽  
Vol 65 (Pt_2) ◽  
pp. 457-462 ◽  
Author(s):  
Juliah Khayeli Akhwale ◽  
Markus Göker ◽  
Manfred Rohde ◽  
Peter Schumann ◽  
Hans-Peter Klenk ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Phenotypic Characteristics ◽  
Optimal Growth Temperature ◽  
Content Type ◽  
Link Type ◽  
Alkaline Lake ◽  
Gene Sequence Analysis ◽  
Lake Elmenteita

A red-pigmented, Gram-reaction-negative, aerobic bacterial strain, designated No.164T, was isolated from sediment sample from the alkaline Lake Elmenteita located in the Kenyan Rift Valley. Results of 16S rRNA gene sequence analysis indicated that the isolate represented a member of the genus Belliella , with the highest sequence similarity (97 %) to Belliella pelovolcani DSM 46698T. Optimal growth temperature was 30–35 °C, at pH 7.0–12.0 in the presence of 0–4 % (w/v) NaCl. Flexirubins were absent. The respiratory menaquinone (MK-7), predominant cellular fatty acids (iso-C15 : 0, anteiso-C15 : 0 and a mixture of C16 : 1ω7c and/or iso-C15 : 0 2-OH) and DNA G+C content (38.1 mol%) of strain No.164T were consistent with those of other members of the genus Belliella . The polar lipids consisted of phosphatidylethanolamine, eight unspecified lipids and one unspecified phospholipid. Several phenotypic characteristics can be used to differentiate this isolate from those of other species of the genus Belliella . The results of polyphasic analyses presented in this study indicated that this isolate should be classified as representing a novel species of the genus Belliella . The name Belliella kenyensis sp. nov. is therefore proposed; the type strain is strain No.164T ( = DSM 46651T = CECT 8551T).

scholarly journals Mahella australiensis gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from an Australian oil well

2004 ◽  
Vol 54 (6) ◽  
pp. 2169-2173 ◽  
Author(s):  
Monica Bonilla Salinas ◽  
Marie-Laure Fardeau ◽  
Pierre Thomas ◽  
Jean-Luc Cayol ◽  
Bharat K. C. Patel ◽  
...  
Keyword(s):  
New Genus ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Growth Conditions ◽  
Thermophilic Bacterium ◽  
Rrna Gene ◽  
Oil Well ◽  
Moderately Thermophilic ◽  
Pyruvate Fermentation ◽  
Bacterium Strain

A novel Gram-positive, anaerobic and moderately thermophilic bacterium, strain 50-1 BONT, was isolated from an Australian terrestrial oil reservoir. Cells were spore-forming straight rods, motile by peritrichous flagella. The optimum growth conditions were 50 °C, pH 7·5 and 0·1 % NaCl. Strain 50-1 BONT fermented arabinose, cellobiose, fructose, galactose, glucose, mannose, sucrose, xylose and yeast extract. Glucose was fermented mainly into lactate, formate, hydrogen and CO2. The major end product of pyruvate fermentation was acetate together with H2 and CO2. Thiosulfate, sulfate, elemental sulfur and nitrate were not used as terminal electron acceptors. The DNA G+C content was 55·5 mol%. The closest phylogenetic relative of strain 50-1 BONT was Thermoanaerobacterium thermosulfurigenes (16S rRNA gene sequence similarity of 85·7 %). As strain 50-1 BONT was physiologically and phylogenetically different from members of the order ‘Thermoanaerobacteriales’, it is proposed that strain 50-1 BONT (=DSM 15567T=CIP 107919T) be classified as the type strain of a novel species of a new genus, Mahella australiensis gen. nov., sp. nov.

scholarly journals Dysgonomonas alginatilytica sp. nov., an alginate-degrading bacterium isolated from a microbial consortium

2015 ◽  
Vol 65 (Pt_10) ◽  
pp. 3570-3575 ◽  
Author(s):  
Akihisa Kita ◽  
Toyokazu Miura ◽  
Yoshiko Okamura ◽  
Tsunehiro Aki ◽  
Yukihiko Matsumura ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Microbial Consortium ◽  
Novel Species ◽  
Sequence Similarity ◽  
Optimal Growth ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Optimal Growth Temperature ◽  
Fermentation Products ◽  
Degrading Bacterium

Gram-stain-negative, facultatively anaerobic, non-motile, non-spore-forming, rod-shaped bacterium, designated strain HUA-2T, was isolated from an alginate-degrading microbial consortium. Strain HUA-2T was related to Dysgonomonas capnocytophagoides JCM 16697T, Dysgonomonas macrotermitis JCM 19375T and Dysgonomonas mossii CCUG 43457T with 95.1 %, 94.1 % and 92.1 % 16S rRNA gene sequence similarity, respectively. The optimal growth temperature and pH for strain HUA-2T were 35 °C and pH 8.0, respectively. Enzyme production, major fermentation products from glucose, and the major cellular fatty acids were different from those of D. capnocytophagoides CCUG 17966T or other members of the genus Dysgonomonas. Therefore, strain HUA-2T is proposed to represent a novel species of the genus Dysgonomonas, for which we propose the name Dysgonomonas alginatilytica sp. nov. The type strain is HUA-2T ( = DSM 100214T = HUT 8134T).

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