scholarly journals Streptomyces sanyensis sp. nov., isolated from mangrove sediment

2011 ◽  
Vol 61 (7) ◽  
pp. 1632-1637 ◽  
Author(s):  
Jin-Lei Sui ◽  
Xiao-Xiong Xu ◽  
Zhi Qu ◽  
Hai-Long Wang ◽  
Hai-Peng Lin ◽  
...  
Keyword(s):  
16S Rrna ◽  
Type Strain ◽  
Novel Species ◽  
Aerial Mycelium ◽  
Mangrove Sediment ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Type Strains

A novel strain, 219820T, whose metabolites were found to be active against tumour cells, was isolated and characterized. The isolate belonged to the genus Streptomyces and had white to grey aerial mycelium and long chains of smooth spores in the aerial mycelium. A phylogenetic tree based on 16S rRNA gene sequences showed that strain 219820T had highest similarity to members of the genus Streptomyces and was most closely, albeit loosely, associated with Streptomyces crystallinus NBRC 15401T (98.624 % similarity), Streptomyces melanogenes NBRC 12890T (98.565 %) and Streptomyces noboritoensis NBRC 13065T (98.564 %). However, DNA–DNA relatedness and phenotypic data readily distinguished strain 219820T from these phylogenetically related type strains. It is evident from the combination of genotypic and phenotypic data that strain 219820T represents a novel species of the genus Streptomyces, for which the name Streptomyces sanyensis sp. nov. is proposed; the type strain is 219820T ( = CGMCC 4.5626T  = DSM 42014T).

scholarly journals Isoptericola chiayiensis sp. nov., isolated from mangrove soil

2011 ◽  
Vol 61 (7) ◽  
pp. 1667-1670 ◽  
Author(s):  
M. Tseng ◽  
H. C. Liao ◽  
W. P. Chiang ◽  
G. F. Yuan
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
The Novel ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Mangrove Soil ◽  
Low Levels ◽  
Type Strains

A novel actinomycete, designated strain 06182M-1T, was isolated from a mangrove soil sample collected from Chiayi County in Taiwan. Phylogenetic analysis based on 16S rRNA gene sequences revealed levels of similarity of 97.0–98.8 % to the type strains of recognized species of the genus Isoptericola. Chemotaxonomic data also supported the placement of strain 06182M-1T within the genus Isoptericola. However, the low levels of DNA–DNA relatedness between the novel strain and the type strains of recognized species of the genus Isoptericola, in combination with differential phenotypic data, demonstrate that strain 06182M-1T represents a novel species of the genus Isoptericola, for which the name Isoptericola chiayiensis sp. nov. is proposed. The type strain is 06182M-1T ( = BCRC 16888T  = KCTC 19740T).

scholarly journals Streptomyces hyderabadensis sp. nov., an actinomycete isolated from soil

2011 ◽  
Vol 61 (1) ◽  
pp. 76-80 ◽  
Author(s):  
T. V. K. Reddy ◽  
Shaik Mahmood ◽  
Laskaris Paris ◽  
Y. Harish Kumar Reddy ◽  
E. M. H. Wellington ◽  
...  
Keyword(s):  
Type Strain ◽  
Andhra Pradesh ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Streptomyces Olivaceus ◽  
Type Strains

A novel actinomycete, designated strain OU-40T, was isolated from farm soil collected from the Hyderabad region of Andhra Pradesh, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of species of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain OU-40T belonged to the genus Streptomyces, and was related most closely to Streptomyces pactum NBRC 13433T (99.0 % sequence similarity), Streptomyces olivaceus NBRC 12805T (99.0 %) and Streptomyces parvulus NBRC 13193T (98.8 %). Strain OU-40T could be distinguished from the type strains of its closest phylogenetic relatives based on levels of DNA–DNA relatedness and comparison of morphological and phenotypic data. It is therefore concluded that strain OU-40T represents a novel species of the genus Streptomyces, for which the name Streptomyces hyderabadensis sp. nov. is proposed. The type strain is OU-40T (=CCTCC AA 209024T =PCM 2692T).

scholarly journals Streptomyces osmaniensis sp. nov., isolated from soil

2010 ◽  
Vol 60 (8) ◽  
pp. 1755-1759 ◽  
Author(s):  
T. V. K. Reddy ◽  
Shaik Mahmood ◽  
M. Mohammed Idris ◽  
Slawomir Ciesielski
Keyword(s):  
Phylogenetic Analysis ◽  
Type Strain ◽  
Novel Species ◽  
Garden Soil ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
The Mean ◽  
Type Strains

A novel actinomycete, designated strain OU-63T, was isolated from garden soil collected on the campus of Osmania University in Hyderabad, southern India. The strain was found to have morphological and chemotaxonomic characteristics typical of the genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the strain belonged to the genus Streptomyces, and was related most closely to Streptomyces chartreusis NBRC 12753T (98.6 % similarity). However, the mean level of DNA–DNA relatedness between the two strains was only 25±1.73 %. Based on DNA–DNA relatedness, morphological and phenotypic data, strain OU-63T could be distinguished from the type strains of phylogenetically related species. It is therefore considered to represent a novel species of the genus Streptomyces, for which the name Streptomyces osmaniensis sp. nov. is proposed. The type strain is OU-63T (=CCTCC AA209025T =PCM 2690T).

scholarly journals Knoellia flava sp. nov., isolated from pig manure

2012 ◽  
Vol 62 (2) ◽  
pp. 384-389 ◽  
Author(s):  
Xiang Yu ◽  
Yan Du ◽  
Gejiao Wang
Keyword(s):  
Fatty Acids ◽  
Genomic Dna ◽  
Type Strain ◽  
Novel Species ◽  
Diaminopimelic Acid ◽  
Pig Manure ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Type Strains

A Gram-positive, aerobic, non-spore-forming actinobacterial strain, designated strain TL1T, was isolated from pig manure in Wuhan, China. The cell wall peptidoglycan contained meso-diaminopimelic acid. The major polar lipids were phosphatidylethanolamine, phosphatidylinositol and diphosphatidylglycerol. The predominant menaquinone was MK-8(H4). The major fatty acids (>10 %) were iso-C16 : 0, iso-C15 : 0 and C17 : 1ω8c. The genomic DNA G+C content was 70.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain TL1T was most closely related to the type strains of Knoellia sinensis (98.5 %), Knoellia subterranea (98.2 %) and Knoellia aerolata (96.9 %). DNA–DNA relatedness values of strain TL1T with the type strains of K. sinensis and K. subterranea were 27.3 and 34.0 %, respectively. Comparison of phenotypic, chemotaxonomic and phylogenetic characteristics among strain TL1T and related organisms revealed that the isolate represents a novel species of the genus Knoellia, for which the name Knoellia flava sp. nov. is proposed; the type strain is TL1T ( = CGMCC 1.10749T = KCTC 19810T).

scholarly journals Maribacter confluentis sp. nov., isolated from the junction between the ocean and a freshwater spring

2015 ◽  
Vol 65 (Pt_9) ◽  
pp. 3079-3085 ◽  
Author(s):  
Sooyeon Park ◽  
Yong-Taek Jung ◽  
Ji-Min Park ◽  
Sung-Min Won ◽  
Jung-Hoon Yoon
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Rrna Gene ◽  
Gram Stain ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Dna Relatedness ◽  
Freshwater Spring ◽  
Type Strains ◽  
Sequence Similarities

A Gram-stain-negative, aerobic, non-spore-forming, non-flagellated, non-gliding and rod-shaped bacterial strain, SSK2-2T, was isolated from the place where the ocean and a freshwater spring meet at Jeju island, South Korea. Strain SSK2-2T grew optimally at 30 °C, at pH 7.0–8.0 and in the presence of 2.0–3.0 % (w/v) NaCl. The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences revealed that strain SSK2-2T fell within the clade comprising the type strains of species of the genus Maribacter, joining the type strain of Maribacter sedimenticola with which it shared 99.4 % similarity. Sequence similarities to the type strains of other Maribacter species were 94.6–98.2 %. Strain SSK2-2T contained MK-6 as the predominant menaquinone and iso-C17 : 0 3-OH, iso-C15 : 0, iso-C15 : 1 G, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c) and summed feature 9 (iso-C17 : 1ω9c and/or 10-methyl C16 : 0) as the major fatty acids. The major polar lipids of strain SSK2-2T were phosphatidylethanolamine and one unidentified lipid. The DNA G+C content of strain SSK2-2T was 38.2 mol% and mean levels of DNA–DNA relatedness with the type strains of four phylogenetically related species of the genus Maribacter were 11–24 %. Differential phenotypic properties, together with phylogenetic and genetic distinctiveness, revealed that strain SSK2-2T is separate from other Maribacter species. On the basis of the data presented, strain SSK2-2T is considered to represent a novel species of the genus Maribacter, for which the name Maribacter confluentis sp. nov. is proposed. The type strain is SSK2-2T ( = KCTC 42604T = CECT 8869T).

scholarly journals Aquimarina macrocephali sp. nov., isolated from sediment adjacent to sperm whale carcasses

2010 ◽  
Vol 60 (10) ◽  
pp. 2298-2302 ◽  
Author(s):  
Masayuki Miyazaki ◽  
Yuriko Nagano ◽  
Yoshihiro Fujiwara ◽  
Yuji Hatada ◽  
Yuichi Nogi
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Sperm Whale ◽  
Gliding Motility ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
Definition Of ◽  
Type Strains

A Gram-negative, rod-shaped, non-spore-forming, strictly aerobic strain with gliding motility, designated JAMB N27T, was isolated from sediment adjacent to sperm whale carcasses off Kagoshima, Japan, at a depth of 219 m. Strain JAMB N27T contained MK-6 as the major isoprenoid quinone and iso-C15 : 0, iso-C15 : 1, C16 : 1 and iso-C17 : 1 as the predominant fatty acids. Casein, chitin, gelatin and starch were degraded. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain JAMB N27T represented a separate lineage within the genus Aquimarina. The DNA G+C content of strain JAMB N27T was 33.1 mol%. DNA–DNA relatedness values between strain JAMB N27T and type strains of species of the genus Aquimarina were significantly lower than the cut-off value accepted for the definition of a novel species. Therefore, strain JAMB N27T represents a novel species, for which the name Aquimarina macrocephali sp. nov. is proposed. The type strain is JAMB N27T (=JCM 15542T=NCIMB 14508T).

scholarly journals Dactylosporangium luridum sp. nov., Dactylosporangium luteum sp. nov. and Dactylosporangium salmoneum sp. nov., nom. rev., isolated from soil

2010 ◽  
Vol 60 (8) ◽  
pp. 1813-1823 ◽  
Author(s):  
Byung-Yong Kim ◽  
James E. M. Stach ◽  
Hang-Yeon Weon ◽  
Soon-Wo Kwon ◽  
Michael Goodfellow
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Morphological Properties ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Properties ◽  
Phenotypic Data ◽  
Gene Sequence Analysis ◽  
Type Strains

Forty strains isolated from soil taken from a hay meadow were assigned to the genus Dactylosporangium on the basis of colonial properties. 16S rRNA gene sequence analysis showed that the isolates formed a group that was most closely related to the type strain of Dactylosporangium aurantiacum, but well separated from other Dactylosporangium type strains and from ‘Dactylosporangium salmoneum’ NRRL B-16294. Twelve of 13 representative isolates had identical 16S rRNA gene sequences and formed a subclade that was distinct from corresponding phyletic lines composed of the remaining isolate, strain BK63T, the ‘D. salmoneum’ strain and the type strains of recognized Dactylosporangium species. DNA–DNA relatedness data indicated that representatives of the multi-membered 16S rRNA gene subclade, isolate BK63T and the ‘D. salmoneum’ subclade formed distinct genomic species; all of these organisms had chemotaxonomic and morphological properties consistent with their classification in the genus Dactylosporangium. They were also distinguished from one another and from the type strains of recognized Dactylosporangium species based on a range of phenotypic properties. Combined genotypic and phenotypic data showed that isolate BK63T, isolates BK51T, BK53 and BK69, and strain NRRL B-16294T should be classified in the genus Dactylosporangium as representing novel species. The names proposed for these species are Dactylosporangium luridum sp. nov. (type strain BK63T  = DSM 45324T  = KACC 20933T  = NRRL B-24775T), Dactylosporangium luteum sp. nov. (type strain BK51T  = DSM 45323T  = KACC 20899T  = NRRL B-24774T) and Dactylosporangium salmoneum sp. nov., nom. rev. (type strain NRRL B-16294T  = ATCC 31222T  = DSM 43910T  = JCM 3272T  = NBRC 14103T).

scholarly journals Hymenobacter yonginensis sp. nov., isolated from a mesotrophic artificial lake

2011 ◽  
Vol 61 (7) ◽  
pp. 1511-1514 ◽  
Author(s):  
Yochan Joung ◽  
Sung-Heun Cho ◽  
Haneul Kim ◽  
Seung Bum Kim ◽  
Kiseong Joh
Keyword(s):  
Fatty Acids ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Artificial Lake ◽  
Type Strains

A non-motile, red-pigmented bacterium, designated strain HMD1010T, was isolated from an artificial lake located within the campus of Hankuk University of Foreign Studies, Yongin, Korea. A phylogenetic tree based on 16S rRNA gene sequences showed that strain HMD1010T formed a lineage within the genus Hymenobacter and was closely related to the type strains of Hymenobacter rigui (96.7 % sequence similarity) and H. gelipurpurascens (95.6 %). The major fatty acids were C16 : 1ω5c (21.9 %), summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c; 17.2 %), iso-C15 : 0 (14.5 %) and summed feature 4 (anteiso-C17 : 1 B and/or iso-C17 : 1 I; 11.9 %). The DNA G+C content was 60.4 mol%. On the basis of the evidence presented in this study, strain HMD1010T represents a novel species of the genus Hymenobacter, for which the name Hymenobacter yonginensis sp. nov. was proposed. The type strain is HMD1010T ( = KCTC 22745T  = CECT 7546T).

Actinomadura meridiana sp. nov., isolated from mountain soil

2012 ◽  
Vol 62 (1) ◽  
pp. 217-222 ◽  
Author(s):  
Soon Dong Lee
Keyword(s):  
Novel Species ◽  
Sequence Similarity ◽  
Aerial Mycelium ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Dna Relatedness ◽  
The Family ◽  
The Media ◽  
Type Strains

A novel actinomycete, designated strain DLS-45T, was isolated from soil from the surface of a rock collected from the peak of Darangshi Oreum (Small Mountain) in Jeju, Republic of Korea. Substrate mycelium was well developed, but aerial mycelium was scant on most of the media tested. Spherical to subspherical spores on the aerial mycelium were in chains that were arranged in hooks and their surfaces were warty. The combination of morphological and chemical features supported the classification of the new isolate in the genus Actinomadura. The neighbour-joining tree based on 16S rRNA gene sequences showed that the strain belonged to the family Thermomonosporaceae and formed a coherent cluster with Actinomadura pelletieri IMSNU 22169T (98.0 % sequence similarity). The other closest relatives were Actinomadura bangladeshensis 3-46-b(3)T (98.2 %) and Actinomadura chokoriensis 3-25-a(11)T (97.9 %). Levels of DNA–DNA relatedness between strain DLS-45T and the type strains of the phylogenetic relatives were less than 17 %. A battery of phenotypic, genotypic and DNA–DNA relatedness data indicated that strain DLS-45T represented a novel species of the genus Actinomadura, for which the name Actinomadura meridiana sp. nov. is proposed. The type strain is strain DLS-45T ( = KCTC 19558T = DSM 45252T).

scholarly journals Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan

2007 ◽  
Vol 57 (7) ◽  
pp. 1625-1629 ◽  
Author(s):  
Kuan-Yin Lin ◽  
Shih-Yi Sheu ◽  
Poh-Shing Chang ◽  
Jang-Cheon Cho ◽  
Wen-Ming Chen
Keyword(s):  
Type Strain ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Phenotypic Data ◽  
Dna Relatedness ◽  
Evolutionary Radiation ◽  
Southern Taiwan ◽  
Monophyletic Branch

A short-rod-shaped, Gram-negative, non-motile bacterial strain, designated AZO-CT, was isolated from a sample of seawater collected from the Eluanbi coast of Pingtung County in southern Taiwan and was characterized by using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences showed that the strain formed a monophyletic branch at the periphery of the evolutionary radiation occupied by the genus Oceanicola in the order Rhodobacterales of the Alphaproteobacteria. The closest neighbours were Oceanicola batsensis HTCC2597T (95.6 % similarity), Oceanicola nanhaiensis SS011B1-20T (94.5 %) and Oceanicola granulosus HTCC2516T (94.0 %). The predominant fatty acid was 18 : 1ω7c (49.1 %), and significant amounts of 19 : 0 cyclo (24.6 %) and 16 : 0 (14.7 %) were present. The DNA–DNA relatedness of the strain with respect to recognized species of the genus Oceanicola was less than 70 %. The isolate was also distinguishable from members of the genus Oceanicola on the basis of phenotypic and biochemical characteristics. It is evident from the genotypic, chemotaxonomic and phenotypic data, therefore, that strain AZO-CT represents a novel species of the genus Oceanicola, for which the name Oceanicola marinus sp. nov. is proposed. The type strain is AZO-CT (=LMG 23705T=BCRC 17591T).

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